Restriction map for
pF1KB6223
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# NO CUT ENZYMES ( 132 enzymes) #
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Acc65I AccI AccIII AclI AflII
AgeI AhdI Alw44I AlwI AlwNI
AocI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BcgI BciVI BclI
BfrI BglI BglII BlnI BpuAI
BsaAI BsaBI BsaI BseAI BsiWI
BsoBI Bsp1407I BspDI BspEI BspMI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DraII DraIII DrdI Eam1105I Eco81I
EcoO109I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MflI MluI MroI MspA1I MunI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 980 GACGTC GACGT^C
AccII 60 CGCG CG^CG
AcyI 980 GACGTC G[GA]^CG[CT]C
AfaI 159 GTAC GT^AC
AflIII 189 ACATGT A^C[GA][CT]GT
Aor51HI 1625 AGCGCT AGC^GCT
AvaII 573 GGACC G^G[AT]CC
BalI 101 TGGCCA TGG^CCA
BbvCI 63 GCTGAGG CCTCAGC(-5/-2)
BcnI 9 CCCGG CC^[GC]GG
BlpI 358 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 358 GCTGAGC GC^T[ACGT]AGC
BsaHI 980 GACGTC G[GA]^CG[CT]C
BsmBI 978 GAGACG CGTCTC(1/5)
BspHI 1002 TCATGA T^CATGA
BspLU11I 189 ACATGT A^CATGT
BsrBI 21 CCGCTC CCGCTC(-3/-3)
BsrFI 511 ACCGGC [GA]^CCGG[CT]
BstAPI 866 GCAAAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 60 CGCG CG^CG
CelII 358 GCTGAGC GC^T[ACGT]AGC
Cfr10I 511 ACCGGC [GA]^CCGG[CT]
Cfr13I 573 GGACC G^G[ACGT]CC
DpnII 931 GATC ^GATC
EagI 15 CGGCCG C^GGCCG
EclXI 15 CGGCCG C^GGCCG
Eco47III 1625 AGCGCT AGC^GCT
Eco52I 15 CGGCCG C^GGCCG
EcoNI 476 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 213 CCATGG C^C[AT][AT]GG
HaeII 1625 AGCGCT [GA]GCGC^[CT]
HgaI 999 GCGTC GACGC(5/10)
Hin1I 980 GACGTC G[GA]^CG[CT]C
HindIII 689 AAGCTT A^AGCTT
MaeII 981 ACGT A^CGT
MboI 931 GATC ^GATC
MluNI 101 TGGCCA TGG^CCA
MscI 101 TGGCCA TGG^CCA
MseI 317 TTAA T^TAA
MvnI 60 CGCG CG^CG
NciI 9 CCCGG CC^[GC]GG
NcoI 213 CCATGG C^CATGG
NdeII 931 GATC ^GATC
NheI 1714 GCTAGC G^CTAGC
NspI 189 ACATGT [GA]CATG^[CT]
PflMI 213 CCATGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 516 CTGCAG CTGCA^G
RcaI 1002 TCATGA T^CATGA
RsaI 159 GTAC GT^AC
SapI 469 GAAGAGC GCTCTTC(1/4)
Sau3AI 931 GATC ^GATC
Sau96I 573 GGACC G^G[ACGT]CC
SfcI 516 CTGCAG C^T[GA][CT]AG
StyI 213 CCATGG C^C[AT][AT]GG
Tru9I 317 TTAA T^TAA
Van91I 213 CCATGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BsaWI 1562 ACCGGA [AT]^CCGG[AT]
1646 ACCGGA
BseRI 607 GAGGAG GAGGAG(10/8)
992 GAGGAG
BsiEI 11 CGGTCG CG[GA][CT]^CG
15 CGGCCG
BsiYI 213 CCATGGAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
476 CCTGGAAAAGG
BslI 213 CCATGGAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
476 CCTGGAAAAGG
BsmFI 68 GGGAC GGGAC(10/14)
328 GTCCC
BstNI 201 CCTGG CC^[AT]GG
476 CCTGG
CfoI 61 GCGC GCG^C
1626 GCGC
EaeI 15 CGGCCG [CT]^GGCC[GA]
101 TGGCCA
EcoRII 201 CCTGG ^CC[AT]GG
476 CCTGG
HhaI 61 GCGC GCG^C
1626 GCGC
HinP1I 61 GCGC G^CGC
1626 GCGC
MvaI 201 CCTGG CC^[AT]GG
476 CCTGG
NlaIV 152 GGTTCC GG[ACGT]^[ACGT]CC
609 GGAGCC
PleI 70 GACTC GAGTC(4/5)
142 GAGTC
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AluI ApoI Asp700I
AspHI BanII BbvI BfaI BmyI
BpmI BsaJI BsgI BsiHKAI BsmAI
BsmI Bsp1286I BsrI BssKI BstF5I
Cac8I DdeI DsaI EarI EcoRI
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboII MnlI MslI
MspI MwoI NlaIII SacI ScrFI
SfaNI StuI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I XmnI