Restriction map for
pF1KB6217
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI AlwI
Aor51HI ApoI AscI AseI AsnI
AspEI AspI AviII AvrII BamHI
BbrPI BbvCI BcgI BciVI BfrI
BglII BlnI BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspLU11I BsrDI Bst1107I
BstBI BstYI BstZ17I ClaI CpoI
DraI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT14I EcoT22I
FseI FspI HindIII HpaI MamI
MfeI MflI MluI MroI MunI
NcoI NdeI NheI NotI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SalI
SbfI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI Tth111I XbaI
XhoI XhoII
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# ONE CUT ENZYMES ( 37 enzymes) #
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Alw44I 1234 GTGCAC G^TGCAC
AocI 747 CCTGAGG CC^T[ACGT]AGG
ApaLI 1234 GTGCAC G^TGCAC
Asp700I 1185 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BclI 1451 TGATCA T^GATCA
Bsp1407I 659 TGTACA T^GTACA
BsrBI 333 CCGCTC CCGCTC(-3/-3)
BsrFI 691 GCCGGC [GA]^CCGG[CT]
BsrGI 659 TGTACA T^GTACA
BssHII 1173 GCGCGC G^CGCGC
BssSI 685 CTCGTG CACGAG(-5/-1)
BstAPI 732 GCACCCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1809 GGTCACC G^GT[ACGT]ACC
BstPI 1809 GGTCACC G^GT[ACGT]ACC
Bsu36I 747 CCTGAGG CC^T[ACGT]AGG
Cfr10I 691 GCCGGC [GA]^CCGG[CT]
DraIII 1593 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 349 CTCTTC CTCTTC(1/4)
Eco81I 747 CCTGAGG CC^T[ACGT]AGG
EcoNI 984 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1809 GGTCACC G^GT[ACGT]ACC
FbaI 1451 TGATCA T^GATCA
HgaI 645 GACGC GACGC(5/10)
Ksp632I 349 CTCTTC CTCTTC(1/4)
KspI 1486 CCGCGG CCGC^GG
MaeII 1499 ACGT A^CGT
NaeI 691 GCCGGC GCC^GGC
NgoMIV 691 GCCGGC G^CCGGC
PvuII 856 CAGCTG CAG^CTG
SacII 1486 CCGCGG CCGC^GG
SapI 348 GCTCTTC GCTCTTC(1/4)
SmaI 1223 CCCGGG CCC^GGG
SspBI 659 TGTACA T^GTACA
TfiI 1145 GAATC G^A[AT]TC
XmaCI 1223 CCCGGG C^CCGGG
XmaI 1223 CCCGGG C^CCGGG
XmnI 1185 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 593 GGTACC G^GTACC
929 GGTACC
AcyI 285 GGCGCC G[GA]^CG[CT]C
385 GGCGCC
Asp718I 593 GGTACC G^GTACC
929 GGTACC
AvaI 26 CTCGGG C^[CT]CG[GA]G
1223 CCCGGG
BalI 8 TGGCCA TGG^CCA
1739 TGGCCA
BbeI 285 GGCGCC GGCGC^C
385 GGCGCC
BfaI 149 CTAG C^TAG
209 CTAG
BglI 430 GCCTTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1964 GCCGAGGGGGC
BlpI 69 GCTCAGC GC^T[ACGT]AGC
1885 GCTGAGC
Bpu1102I 69 GCTCAGC GC^T[ACGT]AGC
1885 GCTGAGC
BsaHI 285 GGCGCC G[GA]^CG[CT]C
385 GGCGCC
BsoBI 26 CTCGGG C^[CT]CG[GA]G
1223 CCCGGG
BspHI 116 TCATGA T^CATGA
236 TCATGA
BstXI 389 CCATCCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
680 CCACTCTCGTGG
CelII 69 GCTCAGC GC^T[ACGT]AGC
1885 GCTGAGC
DrdI 544 GACAAGCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1900 GACCTGCTGGTC
Hin1I 285 GGCGCC G[GA]^CG[CT]C
385 GGCGCC
KasI 285 GGCGCC G^GCGCC
385 GGCGCC
KpnI 593 GGTACC GGTAC^C
929 GGTACC
MaeI 149 CTAG C^TAG
209 CTAG
MluNI 8 TGGCCA TGG^CCA
1739 TGGCCA
MscI 8 TGGCCA TGG^CCA
1739 TGGCCA
MseI 605 TTAA T^TAA
1277 TTAA
MslI 681 CACTCTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1305 CATCAGCATG
MspA1I 856 CAGCTG C[AC]G^C[GT]G
1486 CCGCGG
NarI 285 GGCGCC GG^CGCC
385 GGCGCC
NruI 708 TCGCGA TCG^CGA
1696 TCGCGA
PflMI 1118 CCAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1494 CCAGAACGTGG
PpuMI 143 AGGACCC [GA]G^G[AT]CC[CT]
752 GGGTCCC
PstI 157 CTGCAG CTGCA^G
2144 CTGCAG
RcaI 116 TCATGA T^CATGA
236 TCATGA
ScaI 500 AGTACT AGT^ACT
1505 AGTACT
SexAI 168 ACCAGGT A^CC[AT]GGT
1031 ACCAGGT
SfoI 285 GGCGCC GGC^GCC
385 GGCGCC
Tru9I 605 TTAA T^TAA
1277 TTAA
Van91I 1118 CCAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1494 CCAGAACGTGG
XcmI 1071 CCACCCGATGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1629 CCAGCTCTCTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
ApaI AspHI AvaII BanI BanII
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I