Restriction map for
pF1KB6903
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AflIII AhdI Alw44I AlwI
AocI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanII BbeI BbrPI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BsaAI BsaHI BsaI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HindIII HpaI KasI
KspI MaeI MaeII MaeIII MfeI
MflI MluI MluNI MroI MscI
MseI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI SwaI TaqI Tru9I Tsp45I
Tth111I TthHB8I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 245 GGTACC G^GTACC
AccII 100 CGCG CG^CG
AgeI 248 ACCGGT A^CCGGT
AlwNI 55 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 473 AGCGCT AGC^GCT
Asp718I 245 GGTACC G^GTACC
AvaII 546 GGTCC G^G[AT]CC
BanI 245 GGTACC G^G[CT][GA]CC
BbsI 592 GAAGAC GAAGAC(2/6)
BbvCI 334 CCTCAGC CCTCAGC(-5/-2)
BpmI 541 CTCCAG CTGGAG(16/14)
BpuAI 592 GAAGAC GAAGAC(2/6)
BsaBI 460 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 248 ACCGGT [AT]^CCGG[AT]
BsmAI 187 GAGAC GTCTC(1/5)
BsrBI 82 GAGCGG CCGCTC(-3/-3)
BsrFI 248 ACCGGT [GA]^CCGG[CT]
BstUI 100 CGCG CG^CG
Cfr10I 248 ACCGGT [GA]^CCGG[CT]
Cfr13I 546 GGTCC G^G[ACGT]CC
DraII 545 AGGTCCC [GA]G^G[ACGT]CC[CT]
EarI 193 GAAGAG CTCTTC(1/4)
Eco47III 473 AGCGCT AGC^GCT
EcoO109I 545 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 408 CCTTGG C^C[AT][AT]GG
HapII 249 CCGG C^CGG
HgaI 57 GCGTC GACGC(5/10)
HincII 600 GTCAAC GT[CT]^[GA]AC
HindII 600 GTCAAC GT[CT]^[GA]AC
HpaII 249 CCGG C^CGG
KpnI 245 GGTACC GGTAC^C
Ksp632I 193 GAAGAG CTCTTC(1/4)
MamI 460 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 11 CAGCGG C[AC]G^C[GT]G
MspI 249 CCGG C^CGG
MvnI 100 CGCG CG^CG
NspI 170 GCATGC [GA]CATG^[CT]
PflMI 403 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 248 ACCGGT A^CCGGT
PleI 595 GACTC GAGTC(4/5)
PpuMI 545 AGGTCCC [GA]G^G[AT]CC[CT]
Sau96I 546 GGTCC G^G[ACGT]CC
SphI 170 GCATGC GCATG^C
StyI 408 CCTTGG C^C[AT][AT]GG
TfiI 286 GAATC G^A[AT]TC
Van91I 403 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 152 GAATTT [GA]^AATT[CT]
299 AAATTT
AfaI 103 GTAC GT^AC
246 GTAC
AluI 47 AGCT AG^CT
606 AGCT
ApoI 152 GAATTT [GA]^AATT[CT]
299 AAATTT
BseRI 79 GAGGAG GAGGAG(10/8)
361 GAGGAG
BstAPI 54 GCAGCGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
224 GCAAAATCTGC
BstF5I 237 CATCC GGATG(2/0)
320 CATCC
CfoI 23 GCGC GCG^C
474 GCGC
DpnII 198 GATC ^GATC
388 GATC
FokI 237 CATCC GGATG(9/13)
320 CATCC
HaeII 22 AGCGCC [GA]GCGC^[CT]
473 AGCGCT
HaeIII 366 GGCC GG^CC
406 GGCC
HhaI 23 GCGC GCG^C
474 GCGC
HinP1I 23 GCGC G^CGC
474 GCGC
HinfI 286 GAATC G^A[ACGT]TC
595 GACTC
MboI 198 GATC ^GATC
388 GATC
MslI 267 CATGGATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
393 CATCTTCATG
NdeII 198 GATC ^GATC
388 GATC
RsaI 103 GTAC GT^AC
246 GTAC
Sau3AI 198 GATC ^GATC
388 GATC
SfaNI 460 GATGC GCATC(5/9)
486 GCATC
StuI 365 AGGCCT AGG^CCT
405 AGGCCT
TspRI 129 CAGTG CA[GC]TG[ACGT][ACGT]^
551 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AciI BbvI BsaJI BsiYI BslI
BsmFI BsrI BssKI BstNI Cac8I
DdeI EcoRII FauI Fnu4HI HphI
ItaI MboII MnlI MvaI MwoI
NlaIII NlaIV ScrFI TseI Tsp509I