Restriction map for
pF1KB6203
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# NO CUT ENZYMES ( 93 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI Aor51HI ApoI
AscI AseI AsnI Asp718I AspI
AvrII BamHI BbvCI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstYI BstZ17I CelII ClaI
CpoI DraI DraIII Eco47III EcoNI
EcoRI EcoRV FbaI FseI HgaI
HincII HindII HpaI KpnI MamI
MfeI MflI MroI MseI MunI
NdeI NheI NotI NruI PacI
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI ScaI
SexAI SfiI SgrAI SnaBI SpeI
SphI SspBI SspI SwaI Tru9I
Tth111I XbaI XhoII
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcyI 879 GGCGCC G[GA]^CG[CT]C
AfaI 924 GTAC GT^AC
Asp700I 1452 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 879 GGCGCC GGCGC^C
BcgI 477 CGAATCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1774 CTAG C^TAG
BglI 1800 GCCCTTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 879 GGCGCC G[GA]^CG[CT]C
BsiEI 1821 CGGCCG CG[GA][CT]^CG
BsmAI 467 GAGAC GTCTC(1/5)
BspHI 890 TCATGA T^CATGA
BspLU11I 536 ACATGT A^CATGT
BsrDI 1310 GCAATG GCAATG(2/0)
BstBI 1543 TTCGAA TT^CGAA
BstEII 225 GGTGACC G^GT[ACGT]ACC
BstPI 225 GGTGACC G^GT[ACGT]ACC
BstXI 443 CCATCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 859 GACAGCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1821 CGGCCG C^GGCCG
EclXI 1821 CGGCCG C^GGCCG
Eco52I 1821 CGGCCG C^GGCCG
EcoO65I 225 GGTGACC G^GT[ACGT]ACC
EcoT22I 1632 ATGCAT ATGCA^T
HaeII 879 GGCGCC [GA]GCGC^[CT]
Hin1I 879 GGCGCC G[GA]^CG[CT]C
HindIII 1739 AAGCTT A^AGCTT
KasI 879 GGCGCC G^GCGCC
KspI 332 CCGCGG CCGC^GG
MaeI 1774 CTAG C^TAG
NaeI 1319 GCCGGC GCC^GGC
NarI 879 GGCGCC GG^CGCC
NcoI 488 CCATGG C^CATGG
NgoMIV 1319 GCCGGC G^CCGGC
NsiI 1632 ATGCAT ATGCA^T
NspV 1543 TTCGAA TT^CGAA
PaeR7I 1178 CTCGAG C^TCGAG
PvuII 1296 CAGCTG CAG^CTG
RcaI 890 TCATGA T^CATGA
RsaI 924 GTAC GT^AC
SacI 832 GAGCTC GAGCT^C
SacII 332 CCGCGG CCGC^GG
SapI 1707 GCTCTTC GCTCTTC(1/4)
SfoI 879 GGCGCC GGC^GCC
SfuI 1543 TTCGAA TT^CGAA
SmaI 783 CCCGGG CCC^GGG
SmlI 1178 CTCGAG C^T[CT][GA]AG
StuI 1122 AGGCCT AGG^CCT
Tsp509I 1615 AATT ^AATT
XhoI 1178 CTCGAG C^TCGAG
XmaCI 783 CCCGGG C^CCGGG
XmaI 783 CCCGGG C^CCGGG
XmnI 1452 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AhdI 106 GACTGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1393 GACTGGCTGTC
Alw44I 1878 GTGCAC G^TGCAC
1927 GTGCAC
AlwNI 1333 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1482 CAGGTCCTG
AocI 100 CCTGAGG CC^T[ACGT]AGG
1012 CCTGAGG
ApaLI 1878 GTGCAC G^TGCAC
1927 GTGCAC
AspEI 106 GACTGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1393 GACTGGCTGTC
AviII 352 TGCGCA TGC^GCA
1946 TGCGCA
BbrPI 604 CACGTG CAC^GTG
1411 CACGTG
BspMI 527 ACCTGC ACCTGC(4/8)
1362 GCAGGT
BsrFI 370 ACCGGC [GA]^CCGG[CT]
1319 GCCGGC
Bsu36I 100 CCTGAGG CC^T[ACGT]AGG
1012 CCTGAGG
Cfr10I 370 ACCGGC [GA]^CCGG[CT]
1319 GCCGGC
Eam1105I 106 GACTGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1393 GACTGGCTGTC
EarI 1285 CTCTTC CTCTTC(1/4)
1708 CTCTTC
Eco81I 100 CCTGAGG CC^T[ACGT]AGG
1012 CCTGAGG
FspI 352 TGCGCA TGC^GCA
1946 TGCGCA
Ksp632I 1285 CTCTTC CTCTTC(1/4)
1708 CTCTTC
MluI 926 ACGCGT A^CGCGT
1146 ACGCGT
NspI 536 ACATGT [GA]CATG^[CT]
1763 ACATGC
PflMI 588 CCATACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1159 CCAGATTTTGG
PmaCI 604 CACGTG CAC^GTG
1411 CACGTG
PmlI 604 CACGTG CAC^GTG
1411 CACGTG
SbfI 528 CCTGCAGG CCTGCA^GG
1338 CCTGCAGG
Sse8387I 528 CCTGCAGG CCTGCA^GG
1338 CCTGCAGG
TfiI 478 GAATC G^A[AT]TC
1281 GATTC
Van91I 588 CCATACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1159 CCAGATTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AflIII AluI AlwI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XcmI