Restriction map for pF1KB6164
##################################
# NO CUT ENZYMES (  115 enzymes) #
##################################

Acc65I      AccII       AclI        AflII       AgeI        
Alw44I      Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspI        AvaII       AviII       
AvrII       BanI        BbeI        BbrPI       BbsI        
BcgI        BclI        BfrI        BglI        BglII       
BlnI        BpuAI       BsaAI       BsaBI       BsiEI       
BsiWI       BsmI        BspDI       BspLU11I    BspMI       
BsrDI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     ClaI        
CpoI        DraI        DrdI        EagI        EclXI       
Eco47III    Eco52I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
HincII      HindII      HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MunI        
MvnI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        SwaI        
Tth111I     Van91I      XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AatII        1165  GACGTC               GACGT^C
AccI         1012  GTCTAC               GT^[AC][GT]AC
AccIII        539  TCCGGA               T^CCGGA
AcsI          418  AAATTC               [GA]^AATT[CT]
AcyI         1165  GACGTC               G[GA]^CG[CT]C
AflIII       1733  ACGTGT               A^C[GA][CT]GT
AhdI          388  GACTTCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          505  CCTGAGG              CC^T[ACGT]AGG
ApaI          229  GGGCCC               GGGCC^C
ApoI          418  AAATTC               [GA]^AATT[CT]
Asp700I       531  GAACCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         388  GACTTCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI         750  GGATCC               G^GATCC
BciVI        1554  GTATCC               GTATCC(6/5)
BsaHI        1165  GACGTC               G[GA]^CG[CT]C
BsaI         1609  GAGACC               GGTCTC(1/5)
BsaWI         539  TCCGGA               [AT]^CCGG[AT]
BseAI         539  TCCGGA               T^CCGGA
BsmBI         868  GAGACG               CGTCTC(1/5)
Bsp1407I     1736  TGTACA               T^GTACA
BspEI         539  TCCGGA               T^CCGGA
BspHI        1292  TCATGA               T^CATGA
BsrGI        1736  TGTACA               T^GTACA
BssSI         634  CTCGTG               CACGAG(-5/-1)
BstAPI       1255  GCAGAGCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I        505  CCTGAGG              CC^T[ACGT]AGG
CfoI         1759  GCGC                 GCG^C
DraIII        404  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1386  CCATGG               C^C[GA][CT]GG
Eam1105I      388  GACTTCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        505  CCTGAGG              CC^T[ACGT]AGG
HhaI         1759  GCGC                 GCG^C
Hin1I        1165  GACGTC               G[GA]^CG[CT]C
HinP1I       1759  GCGC                 G^CGC
HindIII       304  AAGCTT               A^AGCTT
MroI          539  TCCGGA               T^CCGGA
MslI          472  CATAGCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI         1386  CCATGG               C^CATGG
PaeR7I        755  CTCGAG               C^TCGAG
PstI          298  CTGCAG               CTGCA^G
RcaI         1292  TCATGA               T^CATGA
SacI          895  GAGCTC               GAGCT^C
SapI          949  GAAGAGC              GCTCTTC(1/4)
ScaI          218  AGTACT               AGT^ACT
SspBI        1736  TGTACA               T^GTACA
XcmI         1742  CCATCAAGGCACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          755  CTCGAG               C^TCGAG
XmnI          531  GAACCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

BalI          619  TGGCCA               TGG^CCA
             1220  TGGCCA              
BcnI          816  CCGGG                CC^[GC]GG
             1538  CCCGG               
BlpI          204  GCTCAGC              GC^T[ACGT]AGC
              892  GCTGAGC             
Bpu1102I      204  GCTCAGC              GC^T[ACGT]AGC
              892  GCTGAGC             
BsgI         1343  CTGCAC               GTGCAG(16/14)
             1449  CTGCAC              
BsrBI         337  GAGCGG               CCGCTC(-3/-3)
              760  GAGCGG              
BsrFI        1077  GCCGGT               [GA]^CCGG[CT]
             1484  GCCGGT              
BstYI         750  GGATCC               [GA]^GATC[CT]
              905  AGATCC              
CelII         204  GCTCAGC              GC^T[ACGT]AGC
              892  GCTGAGC             
Cfr10I       1077  GCCGGT               [GA]^CCGG[CT]
             1484  GCCGGT              
DraII         228  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              229  GGGCCCC             
EaeI          619  TGGCCA               [CT]^GGCC[GA]
             1220  TGGCCA              
EcoNI         987  CCTTCACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1050  CCTGGAGAAGG         
EcoO109I      228  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              229  GGGCCCC             
HgaI          543  GACGC                GACGC(5/10)
              171  GCGTC               
MflI          750  GGATCC               [GA]^GATC[CT]
              905  AGATCC              
MluNI         619  TGGCCA               TGG^CCA
             1220  TGGCCA              
MscI          619  TGGCCA               TGG^CCA
             1220  TGGCCA              
MseI          944  TTAA                 T^TAA
             1544  TTAA                
NciI          816  CCGGG                CC^[GC]GG
             1538  CCCGG               
Tru9I         944  TTAA                 T^TAA
             1544  TTAA                
XhoII         750  GGATCC               [GA]^GATC[CT]
              905  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AspHI       AvaI        BanII       BbvCI       BbvI        
BfaI        BmyI        BpmI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
EarI        EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MnlI        MspA1I      
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PleI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     
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