################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## Acc65I AccII AclI AflII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspI AvaII AviII AvrII BanI BbeI BbrPI BbsI BcgI BclI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsiEI BsiWI BsmI BspDI BspLU11I BspMI BsrDI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 1165 GACGTC GACGT^C AccI 1012 GTCTAC GT^[AC][GT]AC AccIII 539 TCCGGA T^CCGGA AcsI 418 AAATTC [GA]^AATT[CT] AcyI 1165 GACGTC G[GA]^CG[CT]C AflIII 1733 ACGTGT A^C[GA][CT]GT AhdI 388 GACTTCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 505 CCTGAGG CC^T[ACGT]AGG ApaI 229 GGGCCC GGGCC^C ApoI 418 AAATTC [GA]^AATT[CT] Asp700I 531 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 388 GACTTCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 750 GGATCC G^GATCC BciVI 1554 GTATCC GTATCC(6/5) BsaHI 1165 GACGTC G[GA]^CG[CT]C BsaI 1609 GAGACC GGTCTC(1/5) BsaWI 539 TCCGGA [AT]^CCGG[AT] BseAI 539 TCCGGA T^CCGGA BsmBI 868 GAGACG CGTCTC(1/5) Bsp1407I 1736 TGTACA T^GTACA BspEI 539 TCCGGA T^CCGGA BspHI 1292 TCATGA T^CATGA BsrGI 1736 TGTACA T^GTACA BssSI 634 CTCGTG CACGAG(-5/-1) BstAPI 1255 GCAGAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 505 CCTGAGG CC^T[ACGT]AGG CfoI 1759 GCGC GCG^C DraIII 404 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1386 CCATGG C^C[GA][CT]GG Eam1105I 388 GACTTCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 505 CCTGAGG CC^T[ACGT]AGG HhaI 1759 GCGC GCG^C Hin1I 1165 GACGTC G[GA]^CG[CT]C HinP1I 1759 GCGC G^CGC HindIII 304 AAGCTT A^AGCTT MroI 539 TCCGGA T^CCGGA MslI 472 CATAGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 1386 CCATGG C^CATGG PaeR7I 755 CTCGAG C^TCGAG PstI 298 CTGCAG CTGCA^G RcaI 1292 TCATGA T^CATGA SacI 895 GAGCTC GAGCT^C SapI 949 GAAGAGC GCTCTTC(1/4) ScaI 218 AGTACT AGT^ACT SspBI 1736 TGTACA T^GTACA XcmI 1742 CCATCAAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 755 CTCGAG C^TCGAG XmnI 531 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### BalI 619 TGGCCA TGG^CCA 1220 TGGCCA BcnI 816 CCGGG CC^[GC]GG 1538 CCCGG BlpI 204 GCTCAGC GC^T[ACGT]AGC 892 GCTGAGC Bpu1102I 204 GCTCAGC GC^T[ACGT]AGC 892 GCTGAGC BsgI 1343 CTGCAC GTGCAG(16/14) 1449 CTGCAC BsrBI 337 GAGCGG CCGCTC(-3/-3) 760 GAGCGG BsrFI 1077 GCCGGT [GA]^CCGG[CT] 1484 GCCGGT BstYI 750 GGATCC [GA]^GATC[CT] 905 AGATCC CelII 204 GCTCAGC GC^T[ACGT]AGC 892 GCTGAGC Cfr10I 1077 GCCGGT [GA]^CCGG[CT] 1484 GCCGGT DraII 228 GGGGCCC [GA]G^G[ACGT]CC[CT] 229 GGGCCCC EaeI 619 TGGCCA [CT]^GGCC[GA] 1220 TGGCCA EcoNI 987 CCTTCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1050 CCTGGAGAAGG EcoO109I 228 GGGGCCC [GA]G^G[ACGT]CC[CT] 229 GGGCCCC HgaI 543 GACGC GACGC(5/10) 171 GCGTC MflI 750 GGATCC [GA]^GATC[CT] 905 AGATCC MluNI 619 TGGCCA TGG^CCA 1220 TGGCCA MscI 619 TGGCCA TGG^CCA 1220 TGGCCA MseI 944 TTAA T^TAA 1544 TTAA NciI 816 CCGGG CC^[GC]GG 1538 CCCGG Tru9I 944 TTAA T^TAA 1544 TTAA XhoII 750 GGATCC [GA]^GATC[CT] 905 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaI BanII BbvCI BbvI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I