##################################
# NO CUT ENZYMES (  202 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BbvI        BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaJI       BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DdeI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        Fnu4HI      FseI        
FspI        HaeII       HaeIII      HgaI        HhaI        
Hin1I       HinP1I      HindIII     HpaI        ItaI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MamI        MfeI        MluI        MluNI       
MroI        MscI        MunI        MvnI        MwoI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TfiI        TseI        Tsp45I      Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AciI           34  GCGG                 CCGC(-3/-1)
AfaI          252  GTAC                 GT^AC
AvaII         405  GGTCC                G^G[AT]CC
BbsI          346  GAAGAC               GAAGAC(2/6)
BcnI          113  CCCGG                CC^[GC]GG
BmyI          110  GTGCCC               G[AGT]GC[ACT]^C
BpuAI         346  GAAGAC               GAAGAC(2/6)
BsmFI         406  GTCCC                GGGAC(10/14)
Bsp1286I      110  GTGCCC               G[AGT]GC[ACT]^C
BspMI         364  ACCTGC               ACCTGC(4/8)
BstXI         431  CCAAAATGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         147  AGATCC               [GA]^GATC[CT]
Cac8I         463  GCCTGC               GC[ACGT]^[ACGT]GC
Cfr13I        405  GGTCC                G^G[ACGT]CC
HapII         114  CCGG                 C^CGG
HincII         91  GTTGAC               GT[CT]^[GA]AC
HindII         91  GTTGAC               GT[CT]^[GA]AC
HinfI          26  GAGTC                G^A[ACGT]TC
HpaII         114  CCGG                 C^CGG
MaeIII        316  GTTAC                ^GT[ACGT]AC
MflI          147  AGATCC               [GA]^GATC[CT]
MslI          396  CACAGCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        398  CAGCTG               C[AC]G^C[GT]G
MspI          114  CCGG                 C^CGG
NciI          113  CCCGG                CC^[GC]GG
NlaIII         55  CATG                 CATG^
NlaIV         405  GGTCCC               GG[ACGT]^[ACGT]CC
PleI           26  GAGTC                GAGTC(4/5)
PvuII         398  CAGCTG               CAG^CTG
RsaI          252  GTAC                 GT^AC
Sau96I        405  GGTCC                G^G[ACGT]CC
SfaNI         460  GATGC                GCATC(5/9)
Tsp509I       183  AATT                 ^AATT
TspRI         351  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoII         147  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AluI          299  AGCT                 AG^CT
              399  AGCT                
BsaI          361  GAGACC               GGTCTC(1/5)
              427  GAGACC              
BsmAI         361  GAGAC                GTCTC(1/5)
              427  GAGAC               
BstF5I        132  CATCC                GGATG(2/0)
              177  CATCC               
BstNI          30  CCTGG                CC^[AT]GG
              125  CCAGG               
EcoRII         30  CCTGG                ^CC[AT]GG
              125  CCAGG               
FokI          132  CATCC                GGATG(9/13)
              177  CATCC               
HphI          424  GGTGA                GGTGA(8/7)
              229  TCACC               
MseI           44  TTAA                 T^TAA
              451  TTAA                
MvaI           30  CCTGG                CC^[AT]GG
              125  CCAGG               
SfcI          287  CTACAG               C^T[GA][CT]AG
              356  CTACAG              
Tru9I          44  TTAA                 T^TAA
              451  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (    9 enzymes) #
#########################################

AlwI        BssKI       DpnII       MboI        MboII       
MnlI        NdeII       Sau3AI      ScrFI