##################################
# NO CUT ENZYMES ( 181 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BcgI
BciVI BclI BcnI BfrI BglII
BlnI BpmI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I Cfr10I
CpoI DpnII DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII HphI KasI
KpnI KspI MaeIII MamI MboI
MfeI MflI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NdeII
NgoMIV NheI NlaIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI Sau3AI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tsp45I Tth111I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AciI 37 CCGC CCGC(-3/-1)
AflIII 240 ACACGT A^C[GA][CT]GT
AspHI 244 GTGCTC G[AT]GC[AT]^C
AvaII 66 GGACC G^G[AT]CC
BbrPI 241 CACGTG CAC^GTG
BbsI 279 GAAGAC GAAGAC(2/6)
BbvCI 286 GCTGAGG CCTCAGC(-5/-2)
BfaI 43 CTAG C^TAG
BglI 430 GCCCTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 100 GCTCAGC GC^T[ACGT]AGC
BmyI 244 GTGCTC G[AGT]GC[ACT]^C
Bpu1102I 100 GCTCAGC GC^T[ACGT]AGC
BpuAI 279 GAAGAC GAAGAC(2/6)
BsaAI 241 CACGTG [CT]AC^GT[GA]
BsiHKAI 244 GTGCTC G[AT]GC[AT]^C
BsiYI 69 CCAGGAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 69 CCAGGAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 244 GTGCTC G[AGT]GC[ACT]^C
BspDI 250 ATCGAT AT^CGAT
BsrDI 96 CATTGC GCAATG(2/0)
BsrI 262 ACTGG ACTGG(1/-1)
BstF5I 291 GGATG GGATG(2/0)
Cac8I 372 GCACGC GC[ACGT]^[ACGT]GC
CelII 100 GCTCAGC GC^T[ACGT]AGC
CfoI 4 GCGC GCG^C
Cfr13I 66 GGACC G^G[ACGT]CC
ClaI 250 ATCGAT AT^CGAT
EarI 359 CTCTTC CTCTTC(1/4)
FokI 291 GGATG GGATG(9/13)
HhaI 4 GCGC GCG^C
HinP1I 4 GCGC G^CGC
Ksp632I 359 CTCTTC CTCTTC(1/4)
MaeI 43 CTAG C^TAG
MseI 14 TTAA T^TAA
PflMI 69 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 405 GAGTC GAGTC(4/5)
PmaCI 241 CACGTG CAC^GTG
PmlI 241 CACGTG CAC^GTG
SapI 358 GCTCTTC GCTCTTC(1/4)
Sau96I 66 GGACC G^G[ACGT]CC
SfaNI 292 GATGC GCATC(5/9)
SmlI 52 CTCAAG C^T[CT][GA]AG
TaqI 251 TCGA T^CGA
Tru9I 14 TTAA T^TAA
Tsp509I 182 AATT ^AATT
TspRI 194 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 251 TCGA T^CGA
Van91I 69 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AfaI 115 GTAC GT^AC
267 GTAC
BsmFI 190 GTCCC GGGAC(10/14)
214 GTCCC
BstXI 122 CCAAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
332 CCAAGCAGATGG
EcoT14I 137 CCAAGG C^C[AT][AT]GG
296 CCAAGG
HaeIII 387 GGCC GG^CC
459 GGCC
MslI 249 CATCGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
393 CATGGAAATG
MspA1I 61 CAGCTG C[AC]G^C[GT]G
284 CAGCTG
PvuII 61 CAGCTG CAG^CTG
284 CAGCTG
RsaI 115 GTAC GT^AC
267 GTAC
StyI 137 CCAAGG C^C[AT][AT]GG
296 CCAAGG
TfiI 30 GAATC G^A[AT]TC
414 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AluI BbvI BsaJI BssKI BstNI
DdeI EcoRII Fnu4HI HinfI ItaI
MaeII MboII MnlI MvaI MwoI
NlaIII ScrFI TseI