Restriction map for
pF1KB6264
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanII BbeI BbrPI BcgI BclI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BsaBI BsaHI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
BspEI BspHI BspLU11I BsrBI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI Bsu36I CelII CpoI DraII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I FbaI FseI FspI HaeII
Hin1I HincII HindII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PflMI PleI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 689 GTATAC GT^[AC][GT]AC
AgeI 1430 ACCGGT A^CCGGT
BanI 125 GGCACC G^G[CT][GA]CC
BbvCI 32 CCTCAGC CCTCAGC(-5/-2)
BcnI 156 CCGGG CC^[GC]GG
BglII 681 AGATCT A^GATCT
BmyI 254 GGGCAC G[AGT]GC[ACT]^C
BsaAI 2152 TACGTG [CT]AC^GT[GA]
BsaI 172 GAGACC GGTCTC(1/5)
Bsp1286I 254 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 501 TGTACA T^GTACA
BspDI 100 ATCGAT AT^CGAT
BsrDI 563 GCAATG GCAATG(2/0)
BsrFI 1430 ACCGGT [GA]^CCGG[CT]
BsrGI 501 TGTACA T^GTACA
Bst1107I 689 GTATAC GTA^TAC
BstZ17I 689 GTATAC GTA^TAC
Cac8I 3 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 395 GCGC GCG^C
Cfr10I 1430 ACCGGT [GA]^CCGG[CT]
ClaI 100 ATCGAT AT^CGAT
DraI 556 TTTAAA TTT^AAA
DraIII 1740 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 250 CCATGG C^C[GA][CT]GG
EcoRV 200 GATATC GAT^ATC
EcoT14I 250 CCATGG C^C[AT][AT]GG
FauI 1916 GCGGG CCCGC(4/6)
HgaI 2285 GCGTC GACGC(5/10)
HhaI 395 GCGC GCG^C
HinP1I 395 GCGC G^CGC
HindIII 383 AAGCTT A^AGCTT
HinfI 1541 GAATC G^A[ACGT]TC
MseI 557 TTAA T^TAA
NciI 156 CCGGG CC^[GC]GG
NcoI 250 CCATGG C^CATGG
PinAI 1430 ACCGGT A^CCGGT
PshAI 2015 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 259 CTGCAG CTGCA^G
SapI 1751 GAAGAGC GCTCTTC(1/4)
SmlI 769 CTCAAG C^T[CT][GA]AG
SspBI 501 TGTACA T^GTACA
StyI 250 CCATGG C^C[AT][AT]GG
TfiI 1541 GAATC G^A[AT]TC
Tru9I 557 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AflIII 951 ACGTGT A^C[GA][CT]GT
2153 ACGTGT
BciVI 346 GTATCC GTATCC(6/5)
369 GTATCC
BsgI 897 GTGCAG GTGCAG(16/14)
2073 GTGCAG
BsmFI 305 GGGAC GGGAC(10/14)
597 GTCCC
BspMI 185 ACCTGC ACCTGC(4/8)
1934 GCAGGT
BstXI 4 CCAGCTAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1656 CCACGAATGTGG
BstYI 681 AGATCT [GA]^GATC[CT]
802 AGATCC
EarI 601 CTCTTC CTCTTC(1/4)
1751 GAAGAG
Ksp632I 601 CTCTTC CTCTTC(1/4)
1751 GAAGAG
MflI 681 AGATCT [GA]^GATC[CT]
802 AGATCC
MwoI 1860 GCTCCTGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
2112 GCTCCAAGTGC
TaqI 101 TCGA T^CGA
2013 TCGA
TthHB8I 101 TCGA T^CGA
2013 TCGA
XhoII 681 AGATCT [GA]^GATC[CT]
802 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AvaII BbsI
BbvI BpmI BpuAI BsaJI BsaWI
BsiYI BslI BsmAI BsmI BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII EcoRI EcoRII EcoT22I Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MslI MspI MvaI NdeI
NdeII NlaIII NlaIV NsiI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI TseI Tsp45I Tsp509I TspRI
XmnI