Restriction map for
pF1KB6245
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# NO CUT ENZYMES ( 91 enzymes) #
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AatII AccIII AclI AflII AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AspEI AviII BbrPI BciVI
BfrI BglI BglII BlpI Bpu1102I
BsaBI BsaI BseAI BsiWI Bsp1407I
BspDI BspEI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FspI HincII HindII HindIII HpaI
Ksp632I MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PflMI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SalI SapI SbfI SexAI
SfiI SfuI SgrAI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Van91I
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# ONE CUT ENZYMES ( 46 enzymes) #
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AflIII 1767 ACATGT A^C[GA][CT]GT
AgeI 472 ACCGGT A^CCGGT
Asp700I 2160 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 339 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1693 CCTAGG C^CTAGG
BalI 1791 TGGCCA TGG^CCA
BbeI 352 GGCGCC GGCGC^C
BcgI 1925 CGAGTTCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 632 TGATCA T^GATCA
BlnI 1693 CCTAGG C^CTAGG
BsaAI 1335 TACGTA [CT]AC^GT[GA]
BsgI 382 GTGCAG GTGCAG(16/14)
BsiEI 68 CGGTCG CG[GA][CT]^CG
BspHI 572 TCATGA T^CATGA
BspLU11I 1767 ACATGT A^CATGT
BstXI 695 CCAATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 1995 TTTAAA TTT^AAA
EaeI 1791 TGGCCA [CT]^GGCC[GA]
EcoNI 1222 CCTCGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 632 TGATCA T^GATCA
FseI 515 GGCCGGCC GGCCGG^CC
KasI 352 GGCGCC G^GCGCC
KspI 194 CCGCGG CCGC^GG
MluNI 1791 TGGCCA TGG^CCA
MscI 1791 TGGCCA TGG^CCA
MslI 1762 CACCCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 516 GCCGGC GCC^GGC
NarI 352 GGCGCC GG^CGCC
NcoI 98 CCATGG C^CATGG
NgoMIV 516 GCCGGC G^CCGGC
PflFI 339 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 472 ACCGGT A^CCGGT
PpuMI 863 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 858 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 572 TCATGA T^CATGA
SacI 830 GAGCTC GAGCT^C
SacII 194 CCGCGG CCGC^GG
ScaI 1651 AGTACT AGT^ACT
SfoI 352 GGCGCC GGC^GCC
SmaI 511 CCCGGG CCC^GGG
SnaBI 1335 TACGTA TAC^GTA
Tth111I 339 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1617 TCTAGA T^CTAGA
XmaCI 511 CCCGGG C^CCGGG
XmaI 511 CCCGGG C^CCGGG
XmnI 2160 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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Acc65I 1276 GGTACC G^GTACC
1964 GGTACC
AccI 1502 GTCTAC GT^[AC][GT]AC
1771 GTCTAC
AcyI 177 GGCGTC G[GA]^CG[CT]C
352 GGCGCC
AlwNI 385 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
418 CAGAAGCTG
AocI 250 CCTCAGG CC^T[ACGT]AGG
771 CCTTAGG
ApaI 427 GGGCCC GGGCC^C
809 GGGCCC
Asp718I 1276 GGTACC G^GTACC
1964 GGTACC
BbsI 1957 GAAGAC GAAGAC(2/6)
2164 GTCTTC
BbvCI 28 CCTCAGC CCTCAGC(-5/-2)
2134 CCTCAGC
BpuAI 1957 GAAGAC GAAGAC(2/6)
2164 GTCTTC
BsaHI 177 GGCGTC G[GA]^CG[CT]C
352 GGCGCC
BsaWI 472 ACCGGT [AT]^CCGG[AT]
742 TCCGGT
BsmBI 116 CGTCTC CGTCTC(1/5)
1464 CGTCTC
Bsu36I 250 CCTCAGG CC^T[ACGT]AGG
771 CCTTAGG
DsaI 98 CCATGG C^C[GA][CT]GG
194 CCGCGG
Eco81I 250 CCTCAGG CC^T[ACGT]AGG
771 CCTTAGG
Hin1I 177 GGCGTC G[GA]^CG[CT]C
352 GGCGCC
KpnI 1276 GGTACC GGTAC^C
1964 GGTACC
MaeII 739 ACGT A^CGT
1336 ACGT
MaeIII 1444 GTCAC ^GT[ACGT]AC
2212 GTCAC
PaeR7I 455 CTCGAG C^TCGAG
661 CTCGAG
PleI 580 GACTC GAGTC(4/5)
656 GACTC
Tsp45I 1444 GTCAC ^GT[GC]AC
2212 GTCAC
XcmI 140 CCAGTGCGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1530 CCAACGCTTTACTGG
XhoI 455 CTCGAG C^TCGAG
661 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BamHI BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII