Restriction map for
pF1KB6194
##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI ApaI AscI AseI
AsnI Asp718I AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BciVI
BglI BlnI BlpI Bpu1102I BsaAI
BsaHI BseAI BsiWI BspDI BspEI
BspMI BsrFI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DrdI
EcoNI EcoO65I FseI FspI Hin1I
HindIII KasI KpnI KspI MfeI
MluI MroI MspA1I MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AflII 909 CTTAAG C^TTAAG
AlwI 631 GGATC GGATC(4/5)
AlwNI 1079 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1021 CCTCAGG CC^T[ACGT]AGG
Aor51HI 870 AGCGCT AGC^GCT
AvaI 2095 CCCGGG C^[CT]CG[GA]G
BanI 439 GGTGCC G^G[CT][GA]CC
BclI 2045 TGATCA T^GATCA
BfaI 1519 CTAG C^TAG
BfrI 909 CTTAAG C^TTAAG
BpmI 1804 CTGGAG CTGGAG(16/14)
BsaBI 129 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 955 GGTCTC GGTCTC(1/5)
BsaWI 942 TCCGGT [AT]^CCGG[AT]
BsgI 285 GTGCAG GTGCAG(16/14)
BsiEI 1954 CGGCCG CG[GA][CT]^CG
BsmBI 403 GAGACG CGTCTC(1/5)
BsoBI 2095 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1336 TGTACA T^GTACA
BspHI 2087 TCATGA T^CATGA
BsrBI 2104 GAGCGG CCGCTC(-3/-3)
BsrDI 578 GCAATG GCAATG(2/0)
BsrGI 1336 TGTACA T^GTACA
BstXI 1769 CCATCCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1021 CCTCAGG CC^T[ACGT]AGG
DraI 700 TTTAAA TTT^AAA
DraIII 1672 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1954 CGGCCG C^GGCCG
EclXI 1954 CGGCCG C^GGCCG
Eco47III 870 AGCGCT AGC^GCT
Eco52I 1954 CGGCCG C^GGCCG
Eco81I 1021 CCTCAGG CC^T[ACGT]AGG
EcoRV 2112 GATATC GAT^ATC
EcoT22I 788 ATGCAT ATGCA^T
FauI 41 GCGGG CCCGC(4/6)
FbaI 2045 TGATCA T^GATCA
HgaI 1323 GACGC GACGC(5/10)
HincII 73 GTTAAC GT[CT]^[GA]AC
HindII 73 GTTAAC GT[CT]^[GA]AC
HpaI 73 GTTAAC GTT^AAC
MaeI 1519 CTAG C^TAG
MaeIII 377 GTGAC ^GT[ACGT]AC
MamI 129 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 788 ATGCAT ATGCA^T
PflMI 1671 CCACTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 2087 TCATGA T^CATGA
SmaI 2095 CCCGGG CCC^GGG
SspBI 1336 TGTACA T^GTACA
StuI 1226 AGGCCT AGG^CCT
Tsp45I 377 GTGAC ^GT[GC]AC
Van91I 1671 CCACTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 2095 CCCGGG C^CCGGG
XmaI 2095 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 706 GTCTAC GT^[AC][GT]AC
1501 GTCTAC
AflIII 1328 ACATGT A^C[GA][CT]GT
1586 ACATGT
AhdI 37 GACTGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1681 GACTGGTGGTC
Alw44I 624 GTGCAC G^TGCAC
1269 GTGCAC
ApaLI 624 GTGCAC G^TGCAC
1269 GTGCAC
AspEI 37 GACTGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1681 GACTGGTGGTC
BalI 830 TGGCCA TGG^CCA
875 TGGCCA
BbsI 303 GAAGAC GAAGAC(2/6)
1180 GTCTTC
BcgI 480 CGAGTTCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1982 GCAGCAATTTCG
BglII 926 AGATCT A^GATCT
1646 AGATCT
BpuAI 303 GAAGAC GAAGAC(2/6)
1180 GTCTTC
BseRI 1750 GAGGAG GAGGAG(10/8)
1753 GAGGAG
BsmI 341 GAATGC GAATGC(1/-1)
351 GAATGC
BspLU11I 1328 ACATGT A^CATGT
1586 ACATGT
BssSI 478 CACGAG CACGAG(-5/-1)
529 CACGAG
BstYI 926 AGATCT [GA]^GATC[CT]
1646 AGATCT
CfoI 432 GCGC GCG^C
871 GCGC
Eam1105I 37 GACTGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1681 GACTGGTGGTC
EcoRI 1192 GAATTC G^AATTC
1999 GAATTC
EcoT14I 1298 CCTTGG C^C[AT][AT]GG
1349 CCAAGG
HaeII 431 AGCGCC [GA]GCGC^[CT]
870 AGCGCT
HhaI 432 GCGC GCG^C
871 GCGC
HinP1I 432 GCGC G^CGC
871 GCGC
HphI 1830 GGTGA GGTGA(8/7)
1969 TCACC
MflI 926 AGATCT [GA]^GATC[CT]
1646 AGATCT
MluNI 830 TGGCCA TGG^CCA
875 TGGCCA
MscI 830 TGGCCA TGG^CCA
875 TGGCCA
MslI 798 CATGAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1770 CATCCGCATG
SacI 262 GAGCTC GAGCT^C
1756 GAGCTC
SapI 1758 GCTCTTC GCTCTTC(1/4)
1836 GCTCTTC
StyI 1298 CCTTGG C^C[AT][AT]GG
1349 CCAAGG
TfiI 112 GAATC G^A[AT]TC
674 GATTC
XhoII 926 AGATCT [GA]^GATC[CT]
1646 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I AspHI AvaII BanII BbvI
BcnI BmyI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MaeII MboI MboII MnlI
MseI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I
XmnI