Restriction map for
pF1KB6179
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BbrPI BbvCI BcgI
BfaI BglII BlnI BsaWI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstXI BstZ17I Bsu36I ClaI CpoI
DraIII DsaI Eam1105I Eco47III Eco81I
EcoRI EcoRV EcoT22I FseI FspI
HgaI HpaI KpnI KspI MaeI
MfeI MluI MluNI MroI MscI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 400 GTCTAC GT^[AC][GT]AC
AccII 575 CGCG CG^CG
AflII 769 CTTAAG C^TTAAG
ApaI 50 GGGCCC GGGCC^C
BamHI 1395 GGATCC G^GATCC
BclI 92 TGATCA T^GATCA
BfrI 769 CTTAAG C^TTAAG
BglI 235 GCCCGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1705 GCTGAGC GC^T[ACGT]AGC
BpmI 1555 CTGGAG CTGGAG(16/14)
Bpu1102I 1705 GCTGAGC GC^T[ACGT]AGC
BsaBI 939 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1492 GAGACC GGTCTC(1/5)
BseRI 72 CTCCTC GAGGAG(10/8)
BsiEI 149 CGGCCG CG[GA][CT]^CG
BsrDI 893 GCAATG GCAATG(2/0)
BsrFI 16 GCCGGC [GA]^CCGG[CT]
BstUI 575 CGCG CG^CG
BstYI 1395 GGATCC [GA]^GATC[CT]
CelII 1705 GCTGAGC GC^T[ACGT]AGC
Cfr10I 16 GCCGGC [GA]^CCGG[CT]
DraI 640 TTTAAA TTT^AAA
EagI 149 CGGCCG C^GGCCG
EclXI 149 CGGCCG C^GGCCG
Eco52I 149 CGGCCG C^GGCCG
EcoNI 135 CCTGGCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1391 CCAAGG C^C[AT][AT]GG
FauI 971 GCGGG CCCGC(4/6)
FbaI 92 TGATCA T^GATCA
HincII 1456 GTCAAC GT[CT]^[GA]AC
HindII 1456 GTCAAC GT[CT]^[GA]AC
HindIII 526 AAGCTT A^AGCTT
MamI 939 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1395 GGATCC [GA]^GATC[CT]
MspA1I 88 CCGCTG C[AC]G^C[GT]G
MvnI 575 CGCG CG^CG
NaeI 16 GCCGGC GCC^GGC
NgoMIV 16 GCCGGC G^CCGGC
PaeR7I 229 CTCGAG C^TCGAG
PpuMI 584 AGGACCT [GA]G^G[AT]CC[CT]
SacI 226 GAGCTC GAGCT^C
SapI 1548 GAAGAGC GCTCTTC(1/4)
SmaI 1517 CCCGGG CCC^GGG
SspI 721 AATATT AAT^ATT
StyI 1391 CCAAGG C^C[AT][AT]GG
XcmI 984 CCATTACATCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 229 CTCGAG C^TCGAG
XhoII 1395 GGATCC [GA]^GATC[CT]
XmaCI 1517 CCCGGG C^CCGGG
XmaI 1517 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 6 GGCGCC G[GA]^CG[CT]C
57 GGCGCC
AflIII 1283 ACACGT A^C[GA][CT]GT
1739 ACACGT
AspHI 226 GAGCTC G[AT]GC[AT]^C
1297 GTGCTC
BbeI 6 GGCGCC GGCGC^C
57 GGCGCC
BciVI 633 GGATAC GTATCC(6/5)
423 GTATCC
BsaAI 299 TACGTG [CT]AC^GT[GA]
1740 CACGTA
BsaHI 6 GGCGCC G[GA]^CG[CT]C
57 GGCGCC
BsgI 1186 CTGCAC GTGCAG(16/14)
256 GTGCAG
BsiHKAI 226 GAGCTC G[AT]GC[AT]^C
1297 GTGCTC
BspMI 258 GCAGGT ACCTGC(4/8)
1440 GCAGGT
BstEII 1036 GGTGACC G^GT[ACGT]ACC
1788 GGTCACC
BstPI 1036 GGTGACC G^GT[ACGT]ACC
1788 GGTCACC
DrdI 1267 GACATTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1489 GACGAGACCGTC
EaeI 149 CGGCCG [CT]^GGCC[GA]
1212 TGGCCG
EarI 280 GAAGAG CTCTTC(1/4)
1548 GAAGAG
EcoO65I 1036 GGTGACC G^GT[ACGT]ACC
1788 GGTCACC
Hin1I 6 GGCGCC G[GA]^CG[CT]C
57 GGCGCC
KasI 6 GGCGCC G^GCGCC
57 GGCGCC
Ksp632I 280 GAAGAG CTCTTC(1/4)
1548 GAAGAG
NarI 6 GGCGCC GG^CGCC
57 GGCGCC
PleI 928 GACTC GAGTC(4/5)
695 GAGTC
PshAI 690 GACCCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1449 GACCCTGGTC
PstI 1306 CTGCAG CTGCA^G
1597 CTGCAG
SfoI 6 GGCGCC GGC^GCC
57 GGCGCC
StuI 1470 AGGCCT AGG^CCT
1571 AGGCCT
TfiI 332 GATTC G^A[AT]TC
1096 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BmyI BpuAI BsaJI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I