Restriction map for
pF1KB6169
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AflIII AgeI Aor51HI ApoI AscI
AseI AsnI Asp700I AvrII BamHI
BbeI BbvCI BcgI BclI BfaI
BfrI BglII BlnI BseAI BsgI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI Bst1107I
BstBI BstYI BstZ17I ClaI CpoI
DraI Eco47III EcoRI EcoRV EcoT22I
FbaI FseI HindIII HpaI KasI
MaeI MfeI MflI MluI MroI
MseI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Tsp509I XbaI
XhoII XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 1052 GGTACC G^GTACC
AccI 433 GTCGAC GT^[AC][GT]AC
AlwNI 251 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 1052 GGTACC G^GTACC
AviII 509 TGCGCA TGC^GCA
BbrPI 295 CACGTG CAC^GTG
BciVI 1063 GGATAC GTATCC(6/5)
BsaAI 295 CACGTG [CT]AC^GT[GA]
BsaBI 582 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 592 ACCGGA [AT]^CCGG[AT]
BsmBI 1073 GAGACG CGTCTC(1/5)
BspMI 1529 GCAGGT ACCTGC(4/8)
BssHII 80 GCGCGC G^CGCGC
BstAPI 239 GCACCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 273 GGTGACC G^GT[ACGT]ACC
BstPI 273 GGTGACC G^GT[ACGT]ACC
BstXI 170 CCAGAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 208 GACGCGGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1308 CCTCCACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 273 GGTGACC G^GT[ACGT]ACC
FspI 509 TGCGCA TGC^GCA
KpnI 1052 GGTACC GGTAC^C
KspI 1579 CCGCGG CCGC^GG
MamI 582 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 267 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1031 CCATGG C^CATGG
NspI 1571 ACATGC [GA]CATG^[CT]
PaeR7I 1620 CTCGAG C^TCGAG
PflMI 1403 CCAGGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 295 CACGTG CAC^GTG
PmlI 295 CACGTG CAC^GTG
SacII 1579 CCGCGG CCGC^GG
SalI 433 GTCGAC G^TCGAC
SapI 780 GCTCTTC GCTCTTC(1/4)
SfcI 1170 CTACAG C^T[GA][CT]AG
Van91I 1403 CCAGGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1268 CCAGTGGCCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1620 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 33 enzymes) #
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AcyI 1483 GACGCC G[GA]^CG[CT]C
1890 GGCGTC
AhdI 1660 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1690 GACCCCAGGTC
Alw44I 58 GTGCAC G^TGCAC
246 GTGCAC
AlwI 303 GATCC GGATC(4/5)
1362 GATCC
ApaLI 58 GTGCAC G^TGCAC
246 GTGCAC
AspEI 1660 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1690 GACCCCAGGTC
AspI 208 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
276 GACCGTGTC
BbsI 772 GAAGAC GAAGAC(2/6)
217 GTCTTC
BpuAI 772 GAAGAC GAAGAC(2/6)
217 GTCTTC
BsaHI 1483 GACGCC G[GA]^CG[CT]C
1890 GGCGTC
BsrBI 1473 CCGCTC CCGCTC(-3/-3)
1282 GAGCGG
BsrFI 157 GCCGGC [GA]^CCGG[CT]
931 GCCGGC
BssSI 1312 CACGAG CACGAG(-5/-1)
1539 CACGAG
Cfr10I 157 GCCGGC [GA]^CCGG[CT]
931 GCCGGC
DraIII 240 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
295 CACGTGGTG
EagI 137 CGGCCG C^GGCCG
191 CGGCCG
Eam1105I 1660 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1690 GACCCCAGGTC
EclXI 137 CGGCCG C^GGCCG
191 CGGCCG
Eco52I 137 CGGCCG C^GGCCG
191 CGGCCG
HaeII 147 GGCGCT [GA]GCGC^[CT]
1676 AGCGCC
Hin1I 1483 GACGCC G[GA]^CG[CT]C
1890 GGCGTC
HincII 433 GTCGAC GT[CT]^[GA]AC
1078 GTTGAC
HindII 433 GTCGAC GT[CT]^[GA]AC
1078 GTTGAC
NaeI 157 GCCGGC GCC^GGC
931 GCCGGC
NgoMIV 157 GCCGGC G^CCGGC
931 GCCGGC
PflFI 208 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
276 GACCGTGTC
PvuII 454 CAGCTG CAG^CTG
1094 CAGCTG
SacI 703 GAGCTC GAGCT^C
739 GAGCTC
SexAI 241 ACCTGGT A^CC[AT]GGT
747 ACCTGGT
SmlI 1620 CTCGAG C^T[CT][GA]AG
1909 CTCAAG
StuI 692 AGGCCT AGG^CCT
1841 AGGCCT
TfiI 1026 GATTC G^A[AT]TC
1176 GATTC
Tth111I 208 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
276 GACCGTGTC
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# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AfaI AluI AocI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BglI
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SmaI StyI TaqI TseI
Tsp45I TspRI TthHB8I XmaCI XmaI