Restriction map for
pF1KB6151
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvrII BanI BbeI BbrPI
BbvCI BciVI BfrI BlnI BsaAI
BsaHI BsaI BsaWI BseAI BsgI
BsiWI BsmAI BsmBI BsmFI BsoBI
BspDI BspEI BspHI BspLU11I BspMI
BssHII BssSI Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoRV
EcoT22I HaeII Hin1I HindIII HpaI
KasI KpnI KspI MluI MroI
MslI NarI NheI NotI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SphI Sse8387I StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AflIII 38 ACGTGT A^C[GA][CT]GT
AlwNI 1579 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1558 GGGCCC GGGCC^C
AviII 88 TGCGCA TGC^GCA
BalI 605 TGGCCA TGG^CCA
BamHI 394 GGATCC G^GATCC
BbsI 1102 GAAGAC GAAGAC(2/6)
BcgI 1039 GCAAGGGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1515 GCCTGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 930 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 930 GCTCAGC GC^T[ACGT]AGC
BpuAI 1102 GAAGAC GAAGAC(2/6)
BsaBI 442 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 21 CGGCCG CG[GA][CT]^CG
Bsp1407I 1463 TGTACA T^GTACA
BsrDI 1725 CATTGC GCAATG(2/0)
BsrGI 1463 TGTACA T^GTACA
BstAPI 1578 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 499 GGTGACC G^GT[ACGT]ACC
BstPI 499 GGTGACC G^GT[ACGT]ACC
BstXI 570 CCAGATCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 930 GCTCAGC GC^T[ACGT]AGC
EagI 21 CGGCCG C^GGCCG
EarI 627 GAAGAG CTCTTC(1/4)
EclXI 21 CGGCCG C^GGCCG
Eco52I 21 CGGCCG C^GGCCG
EcoNI 1338 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 499 GGTGACC G^GT[ACGT]ACC
FauI 1479 CCCGC CCCGC(4/6)
FseI 1347 GGCCGGCC GGCCGG^CC
FspI 88 TGCGCA TGC^GCA
HgaI 57 GCGTC GACGC(5/10)
HphI 499 GGTGA GGTGA(8/7)
Ksp632I 627 GAAGAG CTCTTC(1/4)
MamI 442 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1067 CAATTG C^AATTG
MluNI 605 TGGCCA TGG^CCA
MscI 605 TGGCCA TGG^CCA
MunI 1067 CAATTG C^AATTG
NcoI 458 CCATGG C^CATGG
NdeI 734 CATATG CA^TATG
NruI 60 TCGCGA TCG^CGA
NspI 1028 ACATGC [GA]CATG^[CT]
PflMI 436 CCATTCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 880 GAGCTC GAGCT^C
SpeI 1224 ACTAGT A^CTAGT
SspBI 1463 TGTACA T^GTACA
SspI 657 AATATT AAT^ATT
Van91I 436 CCATTCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1695 CCAGTTGGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BcnI 1511 CCCGG CC^[GC]GG
1615 CCGGG
BfaI 259 CTAG C^TAG
1225 CTAG
BglII 426 AGATCT A^GATCT
572 AGATCT
BsrBI 1480 CCGCTC CCGCTC(-3/-3)
1573 GAGCGG
BsrFI 213 GCCGGC [GA]^CCGG[CT]
1348 GCCGGC
Cfr10I 213 GCCGGC [GA]^CCGG[CT]
1348 GCCGGC
EaeI 21 CGGCCG [CT]^GGCC[GA]
605 TGGCCA
EcoRI 651 GAATTC G^AATTC
867 GAATTC
HincII 1165 GTCAAC GT[CT]^[GA]AC
1610 GTCAAC
HindII 1165 GTCAAC GT[CT]^[GA]AC
1610 GTCAAC
MaeI 259 CTAG C^TAG
1225 CTAG
NaeI 213 GCCGGC GCC^GGC
1348 GCCGGC
NciI 1511 CCCGG CC^[GC]GG
1615 CCGGG
NgoMIV 213 GCCGGC G^CCGGC
1348 GCCGGC
PleI 743 GACTC GAGTC(4/5)
1632 GACTC
PpuMI 833 AGGACCT [GA]G^G[AT]CC[CT]
1538 AGGTCCT
SfaNI 1529 GCATC GCATC(5/9)
1547 GCATC
TfiI 463 GAATC G^A[AT]TC
1293 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BanII
BbvI BclI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EcoO109I
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfcI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII