Restriction map for
pF1KB6148
##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI Alw44I
ApaI ApaLI AscI AseI AsnI
Asp718I AspI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbsI
BbvCI BcgI BclI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BseAI BsgI BsiEI BsiWI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstEII BstPI BstUI
BstZ17I CelII Cfr10I ClaI CpoI
DraIII DrdI EaeI EagI EclXI
Eco52I EcoO65I EcoRV EcoT22I FbaI
FseI FspI Hin1I HindIII HpaI
KasI KpnI KspI MaeII MaeIII
MamI MluI MluNI MroI MscI
MslI MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tsp45I Tth111I
Van91I XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AflII 1468 CTTAAG C^TTAAG
AhdI 835 GACAGACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 527 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 277 CCTCAGG CC^T[ACGT]AGG
Aor51HI 38 AGCGCT AGC^GCT
Asp700I 1843 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 835 GACAGACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 856 GTGCTC G[AT]GC[AT]^C
AvaI 124 CTCGAG C^[CT]CG[GA]G
BanI 895 GGCACC G^G[CT][GA]CC
BciVI 820 GGATAC GTATCC(6/5)
BfrI 1468 CTTAAG C^TTAAG
BglI 643 GCCCTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1464 AGATCT A^GATCT
BmyI 856 GTGCTC G[AGT]GC[ACT]^C
BsaWI 663 ACCGGA [AT]^CCGG[AT]
BsiHKAI 856 GTGCTC G[AT]GC[AT]^C
BsmI 885 GAATGC GAATGC(1/-1)
BsoBI 124 CTCGAG C^[CT]CG[GA]G
Bsp1286I 856 GTGCTC G[AGT]GC[ACT]^C
BspHI 1767 TCATGA T^CATGA
BssSI 401 CACGAG CACGAG(-5/-1)
BstBI 575 TTCGAA TT^CGAA
BstXI 684 CCAGAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1464 AGATCT [GA]^GATC[CT]
Bsu36I 277 CCTCAGG CC^T[ACGT]AGG
DraI 793 TTTAAA TTT^AAA
Eam1105I 835 GACAGACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1477 GAAGAG CTCTTC(1/4)
Eco47III 38 AGCGCT AGC^GCT
Eco81I 277 CCTCAGG CC^T[ACGT]AGG
EcoNI 24 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 753 GAATTC G^AATTC
HgaI 934 GACGC GACGC(5/10)
Ksp632I 1477 GAAGAG CTCTTC(1/4)
MflI 1464 AGATCT [GA]^GATC[CT]
NcoI 1550 CCATGG C^CATGG
NdeI 473 CATATG CA^TATG
NspV 575 TTCGAA TT^CGAA
PaeR7I 124 CTCGAG C^TCGAG
PpuMI 761 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 1767 TCATGA T^CATGA
SfiI 642 GGCCCTGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 575 TTCGAA TT^CGAA
SphI 1674 GCATGC GCATG^C
XbaI 862 TCTAGA T^CTAGA
XhoI 124 CTCGAG C^TCGAG
XhoII 1464 AGATCT [GA]^GATC[CT]
XmnI 1843 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
DraII 20 GGGGCCT [GA]G^G[ACGT]CC[CT]
761 AGGACCT
DsaI 189 CCACGG C^C[GA][CT]GG
1550 CCATGG
EcoO109I 20 GGGGCCT [GA]G^G[ACGT]CC[CT]
761 AGGACCT
EcoT14I 180 CCAAGG C^C[AT][AT]GG
1550 CCATGG
HincII 546 GTCAAC GT[CT]^[GA]AC
1234 GTTGAC
HindII 546 GTCAAC GT[CT]^[GA]AC
1234 GTTGAC
MfeI 336 CAATTG C^AATTG
1565 CAATTG
MspA1I 1090 CCGCTG C[AC]G^C[GT]G
1129 CCGCTG
MunI 336 CAATTG C^AATTG
1565 CAATTG
NspI 90 GCATGT [GA]CATG^[CT]
1674 GCATGC
PstI 890 CTGCAG CTGCA^G
1079 CTGCAG
SfaNI 1717 GATGC GCATC(5/9)
296 GCATC
StuI 650 AGGCCT AGG^CCT
1083 AGGCCT
StyI 180 CCAAGG C^C[AT][AT]GG
1550 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BcnI BfaI
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsmBI BsmFI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MboI MboII MnlI MseI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I