Restriction map for
pF1KB6603
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspHI AvaI AviII
AvrII BamHI BanII BbeI BbrPI
BbvCI BciVI BcnI BfrI BlnI
BlpI BmyI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraII DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI Ksp632I KspI
MaeII MamI MfeI MluI MroI
MslI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TaqI TthHB8I
Van91I XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 138 GGTACC G^GTACC
AlwI 454 GGATC GGATC(4/5)
Asp718I 138 GGTACC G^GTACC
AspI 787 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1138 GGACC G^G[AT]CC
BalI 513 TGGCCA TGG^CCA
BanI 138 GGTACC G^G[CT][GA]CC
BbsI 958 GAAGAC GAAGAC(2/6)
BcgI 558 CGATTACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 723 TGATCA T^GATCA
BglI 34 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 331 AGATCT A^GATCT
BpuAI 958 GAAGAC GAAGAC(2/6)
BseRI 797 GAGGAG GAGGAG(10/8)
BsmFI 95 GGGAC GGGAC(10/14)
BsrDI 434 CATTGC GCAATG(2/0)
BstEII 874 GGTAACC G^GT[ACGT]ACC
BstPI 874 GGTAACC G^GT[ACGT]ACC
BstYI 331 AGATCT [GA]^GATC[CT]
Cac8I 695 GCTTGC GC[ACGT]^[ACGT]GC
Cfr13I 1138 GGACC G^G[ACGT]CC
DdeI 491 CTAAG C^T[ACGT]AG
DsaI 91 CCACGG C^C[GA][CT]GG
EaeI 513 TGGCCA [CT]^GGCC[GA]
EcoO65I 874 GGTAACC G^GT[ACGT]ACC
FbaI 723 TGATCA T^GATCA
KpnI 138 GGTACC GGTAC^C
MflI 331 AGATCT [GA]^GATC[CT]
MluNI 513 TGGCCA TGG^CCA
MscI 513 TGGCCA TGG^CCA
NlaIV 138 GGTACC GG[ACGT]^[ACGT]CC
PflFI 787 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Sau96I 1138 GGACC G^G[ACGT]CC
SfcI 732 CTGTAG C^T[GA][CT]AG
SspI 415 AATATT AAT^ATT
Tth111I 787 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 675 TCTAGA T^CTAGA
XhoII 331 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BbvI 31 GCTGC GCAGC(8/12)
1033 GCTGC
BpmI 37 CTGGAG CTGGAG(16/14)
1214 CTGGAG
BsiYI 1037 CCCAGCCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1042 CCTGGGTCTGG
BslI 1037 CCCAGCCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1042 CCTGGGTCTGG
BspMI 536 ACCTGC ACCTGC(4/8)
1179 ACCTGC
BsrI 123 ACTGG ACTGG(1/-1)
1135 ACTGG
DraI 823 TTTAAA TTT^AAA
1006 TTTAAA
EcoT14I 224 CCAAGG C^C[AT][AT]GG
836 CCAAGG
Fnu4HI 31 GCTGC GC^[ACGT]GC
1033 GCTGC
HaeIII 42 GGCC GG^CC
514 GGCC
HinfI 484 GAATC G^A[ACGT]TC
970 GAATC
ItaI 31 GCTGC GC^[ACGT]GC
1033 GCTGC
ScaI 408 AGTACT AGT^ACT
912 AGTACT
StyI 224 CCAAGG C^C[AT][AT]GG
836 CCAAGG
TfiI 484 GAATC G^A[AT]TC
970 GAATC
TseI 31 GCTGC G^C[AT]GC
1033 GCTGC
Tsp45I 298 GTGAC ^GT[GC]AC
901 GTGAC
TspRI 246 CAGTG CA[GC]TG[ACGT][ACGT]^
941 CACTG
XcmI 836 CCAAGGAAGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1038 CCAGCCTGGGTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AcsI AfaI AluI ApoI BfaI
BsaI BsaJI BsmAI BssKI BstF5I
BstNI DpnII EcoRII FokI HphI
MaeI MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
RsaI Sau3AI ScrFI SfaNI Tru9I
Tsp509I