Restriction map for
pF1KB9394
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII AccI AcsI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
AspEI AspI AvaII AviII AvrII
BalI BamHI BbrPI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaI BsgI
BsiEI BsiWI BsmAI BsmBI Bsp1407I
BspDI BspLU11I BsrGI Bst1107I BstAPI
BstBI BstEII BstNI BstPI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRII EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KspI MaeI MfeI MflI
MluI MluNI MscI MspA1I MunI
MvaI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TfiI Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 610 GGTACC G^GTACC
AccIII 509 TCCGGA T^CCGGA
AclI 643 AACGTT AA^CGTT
AlwNI 571 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 366 GGGCCC GGGCC^C
Asp700I 841 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 610 GGTACC G^GTACC
AvaI 1138 CCCGAG C^[CT]CG[GA]G
BanII 366 GGGCCC G[GA]GC[CT]^C
BbeI 102 GGCGCC GGCGC^C
BbsI 310 GAAGAC GAAGAC(2/6)
BbvCI 1029 GCTGAGG CCTCAGC(-5/-2)
BcgI 198 GCAGGTGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 562 CTCCAG CTGGAG(16/14)
BpuAI 310 GAAGAC GAAGAC(2/6)
BseAI 509 TCCGGA T^CCGGA
BseRI 995 GAGGAG GAGGAG(10/8)
BsoBI 1138 CCCGAG C^[CT]CG[GA]G
BspEI 509 TCCGGA T^CCGGA
BsrBI 36 CCGCTC CCGCTC(-3/-3)
BsrDI 661 GCAATG GCAATG(2/0)
BsrFI 944 GCCGGC [GA]^CCGG[CT]
BssHII 3 GCGCGC G^CGCGC
BssSI 520 CACGAG CACGAG(-5/-1)
BstXI 1005 CCAGTTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 944 GCCGGC [GA]^CCGG[CT]
DraII 365 AGGGCCC [GA]G^G[ACGT]CC[CT]
EaeI 946 CGGCCA [CT]^GGCC[GA]
EcoO109I 365 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 144 CCAAGG C^C[AT][AT]GG
FauI 8 CCCGC CCCGC(4/6)
HgaI 1102 GACGC GACGC(5/10)
HincII 1126 GTCAAC GT[CT]^[GA]AC
HindII 1126 GTCAAC GT[CT]^[GA]AC
KasI 102 GGCGCC G^GCGCC
KpnI 610 GGTACC GGTAC^C
MroI 509 TCCGGA T^CCGGA
MslI 378 CATAAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 944 GCCGGC GCC^GGC
NarI 102 GGCGCC GG^CGCC
NgoMIV 944 GCCGGC G^CCGGC
NspI 473 GCATGC [GA]CATG^[CT]
Psp1406I 643 AACGTT AA^CGTT
PstI 577 CTGCAG CTGCA^G
SapI 54 GCTCTTC GCTCTTC(1/4)
SfcI 577 CTGCAG C^T[GA][CT]AG
SfoI 102 GGCGCC GGC^GCC
SphI 473 GCATGC GCATG^C
StyI 144 CCAAGG C^C[AT][AT]GG
XmnI 841 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 102 GGCGCC G[GA]^CG[CT]C
1102 GACGCC
AluI 443 AGCT AG^CT
1142 AGCT
AlwI 156 GATCC GGATC(4/5)
495 GATCC
AspHI 182 GTGCTC G[AT]GC[AT]^C
523 GAGCAC
BanI 102 GGCGCC G^G[CT][GA]CC
610 GGTACC
BsaBI 495 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
700 GATGTAGATC
BsaHI 102 GGCGCC G[GA]^CG[CT]C
1102 GACGCC
BsaWI 509 TCCGGA [AT]^CCGG[AT]
714 ACCGGA
BsiHKAI 182 GTGCTC G[AT]GC[AT]^C
523 GAGCAC
BsiYI 8 CCCGCCGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
945 CCGGCCAGAGG
BslI 8 CCCGCCGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
945 CCGGCCAGAGG
BsmFI 161 GGGAC GGGAC(10/14)
680 GGGAC
BsmI 924 GAATGC GAATGC(1/-1)
149 GCATTC
BstF5I 739 GGATG GGATG(2/0)
1059 GGATG
Cfr13I 366 GGGCC G^G[ACGT]CC
367 GGCCC
FokI 739 GGATG GGATG(9/13)
1059 GGATG
Hin1I 102 GGCGCC G[GA]^CG[CT]C
1102 GACGCC
HinfI 244 GACTC G^A[ACGT]TC
350 GACTC
MaeII 167 ACGT A^CGT
644 ACGT
MaeIII 452 GTCAC ^GT[ACGT]AC
956 GTGAC
MamI 495 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
700 GATGTAGATC
MseI 623 TTAA T^TAA
832 TTAA
PleI 244 GACTC GAGTC(4/5)
350 GACTC
Sau96I 366 GGGCC G^G[ACGT]CC
367 GGCCC
SfaNI 135 GATGC GCATC(5/9)
750 GATGC
TaqI 627 TCGA T^CGA
993 TCGA
Tru9I 623 TTAA T^TAA
832 TTAA
Tsp45I 452 GTCAC ^GT[GC]AC
956 GTGAC
TthHB8I 627 TCGA T^CGA
993 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccII AciI AfaI BbvI BcnI
BmyI BsaJI Bsp1286I BspHI BspMI
BsrI BssKI BstUI Cac8I CfoI
DdeI DpnII EarI Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI Ksp632I MboI MboII
MnlI MspI MvnI MwoI NciI
NdeII NlaIII NlaIV RcaI RsaI
Sau3AI ScrFI TseI Tsp509I TspRI