Restriction map for
pF1KB5558
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BseAI BsiEI BsiHKAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRV FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII KasI KpnI KspI MamI
MluI MluNI MroI MscI MslI
MspA1I MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 130 GTAGAC GT^[AC][GT]AC
AgeI 36 ACCGGT A^CCGGT
AlwI 1459 GGATC GGATC(4/5)
AseI 685 ATTAAT AT^TAAT
AsnI 685 ATTAAT AT^TAAT
Asp700I 1003 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 1662 GGACC G^G[AT]CC
BciVI 758 GTATCC GTATCC(6/5)
BmyI 1325 GTGCCC G[AGT]GC[ACT]^C
BsaI 33 GAGACC GGTCTC(1/5)
BsaWI 36 ACCGGT [AT]^CCGG[AT]
BseRI 56 CTCCTC GAGGAG(10/8)
Bsp1286I 1325 GTGCCC G[AGT]GC[ACT]^C
BspHI 1017 TCATGA T^CATGA
BsrFI 36 ACCGGT [GA]^CCGG[CT]
BstAPI 505 GCACAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 8 GGTTACC G^GT[ACGT]ACC
BstPI 8 GGTTACC G^GT[ACGT]ACC
BstXI 44 CCAGGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1459 GGATCT [GA]^GATC[CT]
Cfr10I 36 ACCGGT [GA]^CCGG[CT]
DraII 1661 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 152 TGGCCG [CT]^GGCC[GA]
EcoO109I 1661 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 8 GGTTACC G^GT[ACGT]ACC
EcoRI 712 GAATTC G^AATTC
EcoT22I 1338 ATGCAT ATGCA^T
HapII 37 CCGG C^CGG
HincII 1614 GTTAAC GT[CT]^[GA]AC
HindII 1614 GTTAAC GT[CT]^[GA]AC
HpaI 1614 GTTAAC GTT^AAC
HpaII 37 CCGG C^CGG
MfeI 248 CAATTG C^AATTG
MflI 1459 GGATCT [GA]^GATC[CT]
MspI 37 CCGG C^CGG
MunI 248 CAATTG C^AATTG
NsiI 1338 ATGCAT ATGCA^T
NspI 1712 GCATGT [GA]CATG^[CT]
PflMI 907 CCAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 36 ACCGGT A^CCGGT
PpuMI 1661 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 35 GACCGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 685 ATTAAT AT^TAAT
RcaI 1017 TCATGA T^CATGA
SpeI 1144 ACTAGT A^CTAGT
Van91I 907 CCAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1459 GGATCT [GA]^GATC[CT]
XmnI 1003 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AvaI 68 CTCGGG C^[CT]CG[GA]G
107 CCCGAG
BbsI 1003 GAAGAC GAAGAC(2/6)
881 GTCTTC
BbvI 1288 GCTGC GCAGC(8/12)
1594 GCTGC
BfaI 755 CTAG C^TAG
1145 CTAG
BpuAI 1003 GAAGAC GAAGAC(2/6)
881 GTCTTC
BsgI 98 GTGCAG GTGCAG(16/14)
965 GTGCAG
BsmAI 33 GAGAC GTCTC(1/5)
533 GAGAC
BsmFI 1222 GGGAC GGGAC(10/14)
209 GTCCC
BsmI 1443 GAATGC GAATGC(1/-1)
1340 GCATTC
BsoBI 68 CTCGGG C^[CT]CG[GA]G
107 CCCGAG
BspMI 774 ACCTGC ACCTGC(4/8)
1447 GCAGGT
Cac8I 358 GCACGC GC[ACGT]^[ACGT]GC
1179 GCTTGC
Cfr13I 166 GGGCC G^G[ACGT]CC
1662 GGACC
EarI 979 GAAGAG CTCTTC(1/4)
1654 GAAGAG
EcoT14I 371 CCAAGG C^C[AT][AT]GG
1349 CCTTGG
Fnu4HI 1288 GCTGC GC^[ACGT]GC
1594 GCTGC
ItaI 1288 GCTGC GC^[ACGT]GC
1594 GCTGC
Ksp632I 979 GAAGAG CTCTTC(1/4)
1654 GAAGAG
MaeI 755 CTAG C^TAG
1145 CTAG
MaeII 1007 ACGT A^CGT
1107 ACGT
PleI 1354 GACTC GAGTC(4/5)
1362 GACTC
Sau96I 166 GGGCC G^G[ACGT]CC
1662 GGACC
SfcI 365 CTATAG C^T[GA][CT]AG
1619 CTACAG
StyI 371 CCAAGG C^C[AT][AT]GG
1349 CCTTGG
TfiI 996 GAATC G^A[AT]TC
1550 GAATC
TseI 1288 GCTGC G^C[AT]GC
1594 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AcsI AfaI AluI ApoI BpmI
BsaJI BsiYI BslI BsrDI BsrI
BssKI BstF5I BstNI DdeI DpnII
EcoRII FokI HaeIII HinfI HphI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI ScrFI SfaNI TaqI
Tru9I Tsp45I Tsp509I TspRI TthHB8I