Restriction map for
pF1KB5366
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII AccI AclI AflII AflIII
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AseI AsnI AspEI AspI
AviII AvrII BbsI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaI
BsiWI BsmBI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrDI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
DraI DraIII DrdI Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI HphI MaeIII
MamI MfeI MluI MseI MunI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PmeI PshAI PshBI Psp1406I
PvuI RcaI SalI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI StuI
SwaI TfiI Tru9I Tsp45I Tth111I
Van91I XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 69 enzymes) #
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Acc65I 194 GGTACC G^GTACC
AccIII 470 TCCGGA T^CCGGA
AcsI 522 GAATTT [GA]^AATT[CT]
AcyI 3 GGCGCC G[GA]^CG[CT]C
AgeI 456 ACCGGT A^CCGGT
AlwNI 343 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 522 GAATTT [GA]^AATT[CT]
AscI 126 GGCGCGCC GG^CGCGCC
Asp700I 519 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 194 GGTACC G^GTACC
AspHI 238 GAGCTC G[AT]GC[AT]^C
AvaI 497 CTCGAG C^[CT]CG[GA]G
BamHI 294 GGATCC G^GATCC
BanII 238 GAGCTC G[GA]GC[CT]^C
BbeI 3 GGCGCC GGCGC^C
BbrPI 69 CACGTG CAC^GTG
BfaI 539 CTAG C^TAG
BglI 11 GCCGGAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 238 GAGCTC G[AGT]GC[ACT]^C
BpmI 232 CTGGAG CTGGAG(16/14)
BsaAI 69 CACGTG [CT]AC^GT[GA]
BsaHI 3 GGCGCC G[GA]^CG[CT]C
BseAI 470 TCCGGA T^CCGGA
BsgI 340 GTGCAG GTGCAG(16/14)
BsiEI 216 CGGCCG CG[GA][CT]^CG
BsiHKAI 238 GAGCTC G[AT]GC[AT]^C
BsmI 254 GCATTC GAATGC(1/-1)
BsoBI 497 CTCGAG C^[CT]CG[GA]G
Bsp1286I 238 GAGCTC G[AGT]GC[ACT]^C
BspEI 470 TCCGGA T^CCGGA
BsrBI 360 CCGCTC CCGCTC(-3/-3)
CpoI 453 CGGACCG CG^G[AT]CCG
DraII 156 GGGTCCC [GA]G^G[ACGT]CC[CT]
EagI 216 CGGCCG C^GGCCG
EclXI 216 CGGCCG C^GGCCG
Eco52I 216 CGGCCG C^GGCCG
EcoO109I 156 GGGTCCC [GA]G^G[ACGT]CC[CT]
HaeII 3 GGCGCC [GA]GCGC^[CT]
HgaI 151 GCGTC GACGC(5/10)
Hin1I 3 GGCGCC G[GA]^CG[CT]C
KasI 3 GGCGCC G^GCGCC
KpnI 194 GGTACC GGTAC^C
KspI 219 CCGCGG CCGC^GG
MaeI 539 CTAG C^TAG
MroI 470 TCCGGA T^CCGGA
NarI 3 GGCGCC GG^CGCC
PaeR7I 497 CTCGAG C^TCGAG
PinAI 456 ACCGGT A^CCGGT
PmaCI 69 CACGTG CAC^GTG
PmlI 69 CACGTG CAC^GTG
PpuMI 156 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 328 CTGCAG CTGCA^G
PvuII 445 CAGCTG CAG^CTG
RsrII 453 CGGACCG CG^G[AT]CCG
SacI 238 GAGCTC GAGCT^C
SacII 219 CCGCGG CCGC^GG
SapI 570 GCTCTTC GCTCTTC(1/4)
SbfI 327 CCTGCAGG CCTGCA^GG
SfaNI 134 GCATC GCATC(5/9)
SfcI 328 CTGCAG C^T[GA][CT]AG
SfoI 3 GGCGCC GGC^GCC
SmlI 497 CTCGAG C^T[CT][GA]AG
Sse8387I 327 CCTGCAGG CCTGCA^GG
TaqI 498 TCGA T^CGA
Tsp509I 523 AATT ^AATT
TspRI 507 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 498 TCGA T^CGA
XhoI 497 CTCGAG C^TCGAG
XmnI 519 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AfaI 195 GTAC GT^AC
357 GTAC
BalI 65 TGGCCA TGG^CCA
437 TGGCCA
BanI 3 GGCGCC G^G[CT][GA]CC
194 GGTACC
BseRI 235 GAGGAG GAGGAG(10/8)
300 GAGGAG
BsmAI 410 GAGAC GTCTC(1/5)
542 GTCTC
BsmFI 158 GTCCC GGGAC(10/14)
324 GTCCC
BsrI 50 ACTGG ACTGG(1/-1)
435 ACTGG
BssHII 127 GCGCGC G^CGCGC
210 GCGCGC
BstNI 231 CCTGG CC^[AT]GG
383 CCAGG
BstYI 95 AGATCC [GA]^GATC[CT]
294 GGATCC
DdeI 241 CTCAG C^T[ACGT]AG
509 CTGAG
DpnII 96 GATC ^GATC
295 GATC
EcoRII 231 CCTGG ^CC[AT]GG
383 CCAGG
EcoT14I 122 CCAAGG C^C[AT][AT]GG
596 CCAAGG
FauI 141 CCCGC CCCGC(4/6)
148 CCCGC
HinfI 322 GAGTC G^A[ACGT]TC
500 GAGTC
MboI 96 GATC ^GATC
295 GATC
MflI 95 AGATCC [GA]^GATC[CT]
294 GGATCC
MluNI 65 TGGCCA TGG^CCA
437 TGGCCA
MscI 65 TGGCCA TGG^CCA
437 TGGCCA
MslI 201 CACGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
285 CATTTCTGTG
MvaI 231 CCTGG CC^[AT]GG
383 CCAGG
NdeII 96 GATC ^GATC
295 GATC
NlaIII 21 CATG CATG^
462 CATG
PleI 322 GAGTC GAGTC(4/5)
500 GAGTC
RsaI 195 GTAC GT^AC
357 GTAC
Sau3AI 96 GATC ^GATC
295 GATC
StyI 122 CCAAGG C^C[AT][AT]GG
596 CCAAGG
XhoII 95 AGATCC [GA]^GATC[CT]
294 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccII AciI AluI AlwI AvaII
BbvI BcnI BsaJI BsaWI BsiYI
BslI BsrFI BssKI BstF5I BstUI
Cac8I CfoI Cfr10I Cfr13I DsaI
EaeI EarI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII ItaI
Ksp632I MaeII MboII MnlI MspA1I
MspI MvnI MwoI NaeI NciI
NgoMIV NlaIV Sau96I ScrFI TseI