Restriction map for
pF1KB5278
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaII AvrII BamHI BbrPI
BbsI BbvCI BcgI BciVI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FauI FseI
HincII HindII HindIII HpaI KpnI
Ksp632I KspI MfeI MflI MluI
MroI MunI NdeI NotI NruI
NsiI NspI PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 727 GTCTAC GT^[AC][GT]AC
AcsI 561 GAATTC [GA]^AATT[CT]
AcyI 634 GGCGCC G[GA]^CG[CT]C
AlwI 383 GGATC GGATC(4/5)
ApoI 561 GAATTC [GA]^AATT[CT]
AspHI 978 GAGCAC G[AT]GC[AT]^C
BalI 773 TGGCCA TGG^CCA
BanI 634 GGCGCC G^G[CT][GA]CC
BanII 793 GAGCCC G[GA]GC[CT]^C
BbeI 634 GGCGCC GGCGC^C
BclI 404 TGATCA T^GATCA
BglI 875 GCCTCATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 97 CTCCAG CTGGAG(16/14)
BsaBI 804 GATTCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 634 GGCGCC G[GA]^CG[CT]C
BsaI 1009 GAGACC GGTCTC(1/5)
BseRI 106 GAGGAG GAGGAG(10/8)
BsgI 997 CTGCAC GTGCAG(16/14)
BsiHKAI 978 GAGCAC G[AT]GC[AT]^C
BsmFI 3 GGGAC GGGAC(10/14)
BsrDI 491 GCAATG GCAATG(2/0)
BsrFI 685 GCCGGC [GA]^CCGG[CT]
BstBI 806 TTCGAA TT^CGAA
Cfr10I 685 GCCGGC [GA]^CCGG[CT]
Cfr13I 882 GGGCC G^G[ACGT]CC
DsaI 923 CCATGG C^C[GA][CT]GG
EcoRI 561 GAATTC G^AATTC
FbaI 404 TGATCA T^GATCA
HaeII 634 GGCGCC [GA]GCGC^[CT]
Hin1I 634 GGCGCC G[GA]^CG[CT]C
KasI 634 GGCGCC G^GCGCC
MaeII 593 ACGT A^CGT
MamI 804 GATTCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 293 TCTTC GAAGA(8/7)
MluNI 773 TGGCCA TGG^CCA
MscI 773 TGGCCA TGG^CCA
MseI 707 TTAA T^TAA
NaeI 685 GCCGGC GCC^GGC
NarI 634 GGCGCC GG^CGCC
NcoI 923 CCATGG C^CATGG
NgoMIV 685 GCCGGC G^CCGGC
NheI 33 GCTAGC G^CTAGC
NspV 806 TTCGAA TT^CGAA
PaeR7I 779 CTCGAG C^TCGAG
Sau96I 882 GGGCC G^G[ACGT]CC
SfiI 874 GGCCTCATGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 634 GGCGCC GGC^GCC
SfuI 806 TTCGAA TT^CGAA
SmlI 779 CTCGAG C^T[CT][GA]AG
Tru9I 707 TTAA T^TAA
XcmI 509 CCAACAAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 779 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AccII 664 CGCG CG^CG
1096 CGCG
AvaI 628 CCCGAG C^[CT]CG[GA]G
779 CTCGAG
AviII 20 TGCGCA TGC^GCA
614 TGCGCA
BsoBI 628 CCCGAG C^[CT]CG[GA]G
779 CTCGAG
BstUI 664 CGCG CG^CG
1096 CGCG
BstXI 116 CCAATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
320 CCATGTTCCTGG
EaeI 287 CGGCCA [CT]^GGCC[GA]
773 TGGCCA
FspI 20 TGCGCA TGC^GCA
614 TGCGCA
HgaI 1044 GACGC GACGC(5/10)
1097 GCGTC
MvnI 664 CGCG CG^CG
1096 CGCG
NlaIV 634 GGCGCC GG[ACGT]^[ACGT]CC
792 GGAGCC
TaqI 780 TCGA T^CGA
807 TCGA
TthHB8I 780 TCGA T^CGA
807 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AfaI AluI BbvI BcnI
BfaI BmyI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII PleI
RsaI Sau3AI ScrFI SfaNI StyI
TfiI TseI Tsp45I Tsp509I TspRI