Restriction map for
pF1KB5081
##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AhdI Alw44I AocI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BbrPI BbvCI BciVI BfrI
BglII BlnI BseAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BssSI Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII Eam1105I Eco81I EcoRI EcoRV
EcoT22I FseI FspI HindIII HpaI
KpnI MfeI MluI MroI MseI
MslI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SbfI ScaI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AgeI 1391 ACCGGT A^CCGGT
AlwNI 1481 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 251 AGCGCT AGC^GCT
AspHI 538 GTGCTC G[AT]GC[AT]^C
BamHI 1521 GGATCC G^GATCC
BbsI 268 GTCTTC GAAGAC(2/6)
BcgI 329 GCAGCTTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 305 TGATCA T^GATCA
BfaI 1384 CTAG C^TAG
BlpI 1209 GCTCAGC GC^T[ACGT]AGC
BpmI 1298 CTGGAG CTGGAG(16/14)
Bpu1102I 1209 GCTCAGC GC^T[ACGT]AGC
BpuAI 268 GTCTTC GAAGAC(2/6)
BsaAI 1453 TACGTG [CT]AC^GT[GA]
BsaI 807 GGTCTC GGTCTC(1/5)
BsgI 327 GTGCAG GTGCAG(16/14)
BsiHKAI 538 GTGCTC G[AT]GC[AT]^C
BsiWI 1462 CGTACG C^GTACG
BspMI 155 ACCTGC ACCTGC(4/8)
BsrDI 1200 CATTGC GCAATG(2/0)
BssHII 365 GCGCGC G^CGCGC
BstAPI 1582 GCAGGGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 408 GGTCACC G^GT[ACGT]ACC
BstPI 408 GGTCACC G^GT[ACGT]ACC
CelII 1209 GCTCAGC GC^T[ACGT]AGC
DrdI 1378 GACTGGCTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 372 CGGCCG C^GGCCG
EclXI 372 CGGCCG C^GGCCG
Eco47III 251 AGCGCT AGC^GCT
Eco52I 372 CGGCCG C^GGCCG
EcoNI 1294 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 408 GGTCACC G^GT[ACGT]ACC
FbaI 305 TGATCA T^GATCA
KspI 503 CCGCGG CCGC^GG
MaeI 1384 CTAG C^TAG
MaeII 1454 ACGT A^CGT
NaeI 190 GCCGGC GCC^GGC
NgoMIV 190 GCCGGC G^CCGGC
PaeR7I 17 CTCGAG C^TCGAG
PflMI 1179 CCAGTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1391 ACCGGT A^CCGGT
PpuMI 1045 GGGACCT [GA]G^G[AT]CC[CT]
PstI 457 CTGCAG CTGCA^G
SacII 503 CCGCGG CCGC^GG
SapI 1173 GCTCTTC GCTCTTC(1/4)
SfcI 457 CTGCAG C^T[GA][CT]AG
SfiI 180 GGCCGTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tsp509I 1450 AATT ^AATT
Van91I 1179 CCAGTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 17 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 1348 GTCGAC GT^[AC][GT]AC
1387 GTCGAC
ApaI 243 GGGCCC GGGCC^C
1576 GGGCCC
BalI 1031 TGGCCA TGG^CCA
1316 TGGCCA
BsaBI 303 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
684 GATGAACATC
BsaWI 640 ACCGGA [AT]^CCGG[AT]
1391 ACCGGT
BsmAI 20 GAGAC GTCTC(1/5)
808 GTCTC
BsrFI 190 GCCGGC [GA]^CCGG[CT]
1391 ACCGGT
BstXI 413 CCACGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
797 CCATGGTTCTGG
BstYI 555 GGATCT [GA]^GATC[CT]
1521 GGATCC
Cfr10I 190 GCCGGC [GA]^CCGG[CT]
1391 ACCGGT
MamI 303 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
684 GATGAACATC
MflI 555 GGATCT [GA]^GATC[CT]
1521 GGATCC
MluNI 1031 TGGCCA TGG^CCA
1316 TGGCCA
MscI 1031 TGGCCA TGG^CCA
1316 TGGCCA
NcoI 797 CCATGG C^CATGG
945 CCATGG
SalI 1348 GTCGAC G^TCGAC
1387 GTCGAC
SexAI 128 ACCAGGT A^CC[AT]GGT
584 ACCTGGT
SmaI 122 CCCGGG CCC^GGG
273 CCCGGG
SmlI 17 CTCGAG C^T[CT][GA]AG
255 CTTGAG
TfiI 544 GATTC G^A[AT]TC
1612 GAATC
XhoII 555 GGATCT [GA]^GATC[CT]
1521 GGATCC
XmaCI 122 CCCGGG C^CCGGG
273 CCCGGG
XmaI 122 CCCGGG C^CCGGG
273 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaI AvaII BanI BanII
BbeI BbvI BcnI BglI BmyI
BsaHI BsaJI BseRI BsiEI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfoI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XcmI