Restriction map for
pF1KE0285
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# NO CUT ENZYMES ( 161 enzymes) #
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Acc65I AccII AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvrII BalI
BanI BbeI BbrPI BcgI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstBI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI HaeII HapII
HgaI HincII HindII HindIII HinfI
HpaI HpaII KasI KpnI Ksp632I
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MseI MspA1I
MspI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TfiI Tru9I Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 238 GACGTC GACGT^C
AccI 155 GTAGAC GT^[AC][GT]AC
AcyI 238 GACGTC G[GA]^CG[CT]C
AfaI 124 GTAC GT^AC
ApaI 652 GGGCCC GGGCC^C
AspHI 539 GTGCTC G[AT]GC[AT]^C
AviII 378 TGCGCA TGC^GCA
BamHI 112 GGATCC G^GATCC
BanII 652 GGGCCC G[GA]GC[CT]^C
BbvCI 168 GCTGAGG CCTCAGC(-5/-2)
BciVI 513 GGATAC GTATCC(6/5)
BpmI 138 CTGGAG CTGGAG(16/14)
BsaHI 238 GACGTC G[GA]^CG[CT]C
BsaI 92 GGTCTC GGTCTC(1/5)
BsaJI 116 CCATGG C^C[ACGT][ACGT]GG
BseRI 19 CTCCTC GAGGAG(10/8)
BsiHKAI 539 GTGCTC G[AT]GC[AT]^C
BsmAI 93 GTCTC GTCTC(1/5)
BsmFI 596 GTCCC GGGAC(10/14)
Bsp1407I 123 TGTACA T^GTACA
BspHI 321 TCATGA T^CATGA
BsrGI 123 TGTACA T^GTACA
BstAPI 381 GCAAGAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 610 GGTAACC G^GT[ACGT]ACC
BstPI 610 GGTAACC G^GT[ACGT]ACC
BstYI 112 GGATCC [GA]^GATC[CT]
Cac8I 396 GCGGGC GC[ACGT]^[ACGT]GC
DdeI 169 CTGAG C^T[ACGT]AG
DraIII 643 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 116 CCATGG C^C[GA][CT]GG
EcoO65I 610 GGTAACC G^GT[ACGT]ACC
EcoT14I 116 CCATGG C^C[AT][AT]GG
FauI 396 GCGGG CCCGC(4/6)
FspI 378 TGCGCA TGC^GCA
Hin1I 238 GACGTC G[GA]^CG[CT]C
HphI 300 GGTGA GGTGA(8/7)
MflI 112 GGATCC [GA]^GATC[CT]
NcoI 116 CCATGG C^CATGG
NspI 258 GCATGT [GA]CATG^[CT]
PflMI 642 CCACCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 550 CTGCAG CTGCA^G
RcaI 321 TCATGA T^CATGA
RsaI 124 GTAC GT^AC
SfaNI 588 GCATC GCATC(5/9)
SmlI 542 CTCAAG C^T[CT][GA]AG
SspBI 123 TGTACA T^GTACA
StyI 116 CCATGG C^C[AT][AT]GG
Tsp45I 301 GTGAC ^GT[GC]AC
Tsp509I 225 AATT ^AATT
TspRI 647 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 642 CCACCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 112 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 31 enzymes) #
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AluI 279 AGCT AG^CT
533 AGCT
AlwI 113 GATCC GGATC(4/5)
112 GGATC
AvaII 143 GGTCC G^G[AT]CC
173 GGTCC
BbsI 78 GAAGAC GAAGAC(2/6)
345 GAAGAC
BbvI 6 GCTGC GCAGC(8/12)
577 GCTGC
BmyI 539 GTGCTC G[AGT]GC[ACT]^C
652 GGGCCC
BpuAI 78 GAAGAC GAAGAC(2/6)
345 GAAGAC
Bsp1286I 539 GTGCTC G[AGT]GC[ACT]^C
652 GGGCCC
BsrI 455 ACTGG ACTGG(1/-1)
646 CCAGT
BssKI 137 CCTGG ^CC[ACGT]GG
499 CCAGG
BstNI 137 CCTGG CC^[AT]GG
499 CCAGG
CfoI 9 GCGC GCG^C
379 GCGC
DpnII 113 GATC ^GATC
660 GATC
DraII 651 GGGGCCC [GA]G^G[ACGT]CC[CT]
652 GGGCCCC
EcoO109I 651 GGGGCCC [GA]G^G[ACGT]CC[CT]
652 GGGCCCC
EcoRII 137 CCTGG ^CC[AT]GG
499 CCAGG
HaeIII 229 GGCC GG^CC
653 GGCC
HhaI 9 GCGC GCG^C
379 GCGC
HinP1I 9 GCGC G^CGC
379 GCGC
MaeII 239 ACGT A^CGT
375 ACGT
MaeIII 301 GTGAC ^GT[ACGT]AC
611 GTAAC
MboI 113 GATC ^GATC
660 GATC
MslI 60 CACCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
177 CATTTGTATG
MvaI 137 CCTGG CC^[AT]GG
499 CCAGG
MwoI 9 GCGCTTTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
381 GCAAGAGTTGC
NdeII 113 GATC ^GATC
660 GATC
Sau3AI 113 GATC ^GATC
660 GATC
ScrFI 137 CCTGG CC^[ACGT]GG
499 CCAGG
TaqI 96 TCGA T^CGA
213 TCGA
TseI 6 GCTGC G^C[AT]GC
577 GCTGC
TthHB8I 96 TCGA T^CGA
213 TCGA
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# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AciI BsiYI BslI BstF5I Cfr13I
Fnu4HI FokI ItaI MboII MnlI
NlaIII NlaIV Sau96I SfcI