Restriction map for
pF1KE0255
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AfaI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BanI
BbeI BbrPI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KasI KpnI KspI
MamI MluI MluNI MscI MspA1I
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AccIII 356 TCCGGA T^CCGGA
AciI 134 CCGC CCGC(-3/-1)
AcyI 202 GACGCC G[GA]^CG[CT]C
AflIII 605 ACACGT A^C[GA][CT]GT
ApaI 269 GGGCCC GGGCC^C
AseI 538 ATTAAT AT^TAAT
AsnI 538 ATTAAT AT^TAAT
AvaI 840 CCCGGG C^[CT]CG[GA]G
BamHI 35 GGATCC G^GATCC
BanII 269 GGGCCC G[GA]GC[CT]^C
BbsI 970 GAAGAC GAAGAC(2/6)
BbvCI 284 CCTCAGC CCTCAGC(-5/-2)
BfaI 427 CTAG C^TAG
BmyI 269 GGGCCC G[AGT]GC[ACT]^C
BpuAI 970 GAAGAC GAAGAC(2/6)
BsaHI 202 GACGCC G[GA]^CG[CT]C
BsaI 991 GGTCTC GGTCTC(1/5)
BsaWI 356 TCCGGA [AT]^CCGG[AT]
BseAI 356 TCCGGA T^CCGGA
BsmAI 992 GTCTC GTCTC(1/5)
BsoBI 840 CCCGGG C^[CT]CG[GA]G
Bsp1286I 269 GGGCCC G[AGT]GC[ACT]^C
BspEI 356 TCCGGA T^CCGGA
BspMI 855 GCAGGT ACCTGC(4/8)
BstEII 1212 GGTCACC G^GT[ACGT]ACC
BstPI 1212 GGTCACC G^GT[ACGT]ACC
BstYI 35 GGATCC [GA]^GATC[CT]
CfoI 820 GCGC GCG^C
DsaI 72 CCATGG C^C[GA][CT]GG
EarI 1231 CTCTTC CTCTTC(1/4)
EcoNI 1203 CCTTTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1212 GGTCACC G^GT[ACGT]ACC
FauI 133 CCCGC CCCGC(4/6)
HaeII 819 GGCGCT [GA]GCGC^[CT]
HgaI 202 GACGC GACGC(5/10)
HhaI 820 GCGC GCG^C
Hin1I 202 GACGCC G[GA]^CG[CT]C
HinP1I 820 GCGC G^CGC
HincII 1249 GTCAAC GT[CT]^[GA]AC
HindII 1249 GTCAAC GT[CT]^[GA]AC
Ksp632I 1231 CTCTTC CTCTTC(1/4)
MaeI 427 CTAG C^TAG
MaeII 607 ACGT A^CGT
MfeI 901 CAATTG C^AATTG
MflI 35 GGATCC [GA]^GATC[CT]
MroI 356 TCCGGA T^CCGGA
MseI 539 TTAA T^TAA
MslI 606 CACGTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 901 CAATTG C^AATTG
NcoI 72 CCATGG C^CATGG
NspI 1170 ACATGC [GA]CATG^[CT]
PflMI 768 CCAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1031 GAGTC GAGTC(4/5)
PpuMI 722 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 538 ATTAAT AT^TAAT
SmaI 840 CCCGGG CCC^GGG
TaqI 398 TCGA T^CGA
Tru9I 539 TTAA T^TAA
TthHB8I 398 TCGA T^CGA
Van91I 768 CCAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 7 CCAGCTTGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 35 GGATCC [GA]^GATC[CT]
XmaCI 840 CCCGGG C^CCGGG
XmaI 840 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AlwNI 512 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
880 CAGACCCTG
BseRI 364 CTCCTC GAGGAG(10/8)
1129 CTCCTC
BsmFI 1102 GGGAC GGGAC(10/14)
724 GTCCC
BstF5I 342 CATCC GGATG(2/0)
702 GGATG
BstXI 242 CCACCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
914 CCAGTCTGTTGG
DpnII 36 GATC ^GATC
294 GATC
FokI 342 CATCC GGATG(9/13)
702 GGATG
HphI 995 TCACC GGTGA(8/7)
1214 TCACC
MboI 36 GATC ^GATC
294 GATC
NdeII 36 GATC ^GATC
294 GATC
PstI 88 CTGCAG CTGCA^G
871 CTGCAG
Sau3AI 36 GATC ^GATC
294 GATC
SfcI 88 CTGCAG C^T[GA][CT]AG
871 CTGCAG
StuI 1125 AGGCCT AGG^CCT
1186 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AluI AlwI AvaII BbvI BcnI
BpmI BsaJI BsiYI BslI BsrI
BssKI BstNI Cac8I Cfr13I DdeI
DraII EcoO109I EcoRII EcoT14I Fnu4HI
HaeIII HapII HinfI HpaII ItaI
MaeIII MboII MnlI MspI MvaI
MwoI NciI NlaIII NlaIV Sau96I
ScrFI SfaNI StyI TfiI TseI
Tsp45I Tsp509I TspRI