Restriction map for
pF1KB6680
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BciVI
BclI BfrI BglII BlnI BpuAI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FspI HaeII Hin1I HincII
HindII HindIII HpaI KasI KspI
MaeIII MfeI MluI MroI MseI
MunI MvnI NarI NdeI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tsp45I Tth111I Van91I XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 419 GGTACC G^GTACC
AlwNI 988 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 744 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 419 GGTACC G^GTACC
AvaII 807 GGACC G^G[AT]CC
BanI 419 GGTACC G^G[CT][GA]CC
BcgI 1220 GCAGCCTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1017 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1017 GCTCAGC GC^T[ACGT]AGC
BsaAI 368 TACGTG [CT]AC^GT[GA]
BsaBI 681 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 295 GGTCTC GGTCTC(1/5)
BsmBI 975 GAGACG CGTCTC(1/5)
BsmI 448 GAATGC GAATGC(1/-1)
BsrDI 308 GCAATG GCAATG(2/0)
BsrFI 766 GCCGGC [GA]^CCGG[CT]
CelII 1017 GCTCAGC GC^T[ACGT]AGC
Cfr10I 766 GCCGGC [GA]^CCGG[CT]
DraII 692 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 797 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 851 CCATGG C^C[GA][CT]GG
EcoO109I 692 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 851 CCATGG C^C[AT][AT]GG
FseI 765 GGCCGGCC GGCCGG^CC
HphI 402 GGTGA GGTGA(8/7)
KpnI 419 GGTACC GGTAC^C
MaeII 369 ACGT A^CGT
MamI 681 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 766 GCCGGC GCC^GGC
NcoI 851 CCATGG C^CATGG
NgoMIV 766 GCCGGC G^CCGGC
NheI 708 GCTAGC G^CTAGC
PaeR7I 1228 CTCGAG C^TCGAG
SapI 481 GAAGAGC GCTCTTC(1/4)
SmaI 524 CCCGGG CCC^GGG
StuI 881 AGGCCT AGG^CCT
StyI 851 CCATGG C^C[AT][AT]GG
XhoI 1228 CTCGAG C^TCGAG
XmaCI 524 CCCGGG C^CCGGG
XmaI 524 CCCGGG C^CCGGG
XmnI 744 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AspHI 484 GAGCTC G[AT]GC[AT]^C
1129 GAGCTC
BalI 503 TGGCCA TGG^CCA
662 TGGCCA
BfaI 146 CTAG C^TAG
709 CTAG
BglI 550 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1243 GCCAAGATGGC
BsiHKAI 484 GAGCTC G[AT]GC[AT]^C
1129 GAGCTC
BsmFI 491 GGGAC GGGAC(10/14)
1011 GTCCC
BsrI 416 ACTGG ACTGG(1/-1)
1047 ACTGG
BstYI 407 AGATCC [GA]^GATC[CT]
1088 AGATCC
CfoI 603 GCGC GCG^C
636 GCGC
HgaI 790 GACGC GACGC(5/10)
977 GACGC
HhaI 603 GCGC GCG^C
636 GCGC
HinP1I 603 GCGC G^CGC
636 GCGC
MaeI 146 CTAG C^TAG
709 CTAG
MflI 407 AGATCC [GA]^GATC[CT]
1088 AGATCC
MluNI 503 TGGCCA TGG^CCA
662 TGGCCA
MscI 503 TGGCCA TGG^CCA
662 TGGCCA
MslI 933 CACAGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1309 CACATCAATG
NlaIV 61 GGTTCC GG[ACGT]^[ACGT]CC
419 GGTACC
PstI 1006 CTGCAG CTGCA^G
1081 CTGCAG
SacI 484 GAGCTC GAGCT^C
1129 GAGCTC
SmlI 577 CTCAAG C^T[CT][GA]AG
1228 CTCGAG
Tsp509I 318 AATT ^AATT
1303 AATT
TspRI 1046 CACTG CA[GC]TG[ACGT][ACGT]^
1328 CAGTG
XhoII 407 AGATCC [GA]^GATC[CT]
1088 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsoBI Bsp1286I BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EaeI
EarI EcoNI EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TseI TthHB8I XcmI