Restriction map for
pF1KB6629
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII AccI AclI AflII AgeI
AlwI AocI Aor51HI AscI AseI
AsnI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BcgI BciVI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaI BsiWI BsmI
BsoBI BspDI BspHI BspLU11I BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HpaI KasI MaeI MaeIII MamI
MfeI MflI MluI MseI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tru9I Tsp45I Tth111I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 23 GGTACC G^GTACC
AccIII 818 TCCGGA T^CCGGA
AcyI 907 GACGCC G[GA]^CG[CT]C
AflIII 857 ACGTGT A^C[GA][CT]GT
AhdI 583 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1237 GTGCAC G^TGCAC
AlwNI 640 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1127 GGGCCC GGGCC^C
ApaLI 1237 GTGCAC G^TGCAC
Asp718I 23 GGTACC G^GTACC
AspEI 583 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1237 GTGCAC G[AT]GC[AT]^C
BalI 455 TGGCCA TGG^CCA
BbsI 1223 GTCTTC GAAGAC(2/6)
BbvCI 139 CCTCAGC CCTCAGC(-5/-2)
BclI 335 TGATCA T^GATCA
BglI 889 GCCAGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1223 GTCTTC GAAGAC(2/6)
BsaHI 907 GACGCC G[GA]^CG[CT]C
BsaWI 818 TCCGGA [AT]^CCGG[AT]
BseAI 818 TCCGGA T^CCGGA
BsgI 1117 CTGCAC GTGCAG(16/14)
BsiEI 993 CGGCCG CG[GA][CT]^CG
BsiHKAI 1237 GTGCAC G[AT]GC[AT]^C
BsmBI 1077 CGTCTC CGTCTC(1/5)
Bsp1407I 434 TGTACA T^GTACA
BspEI 818 TCCGGA T^CCGGA
BspMI 803 ACCTGC ACCTGC(4/8)
BsrDI 84 CATTGC GCAATG(2/0)
BsrFI 26 ACCGGC [GA]^CCGG[CT]
BsrGI 434 TGTACA T^GTACA
Cfr10I 26 ACCGGC [GA]^CCGG[CT]
DrdI 226 GACTACAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 993 CGGCCG C^GGCCG
Eam1105I 583 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 993 CGGCCG C^GGCCG
Eco52I 993 CGGCCG C^GGCCG
EcoT14I 555 CCTTGG C^C[AT][AT]GG
FbaI 335 TGATCA T^GATCA
Hin1I 907 GACGCC G[GA]^CG[CT]C
HincII 1135 GTCAAC GT[CT]^[GA]AC
HindII 1135 GTCAAC GT[CT]^[GA]AC
HindIII 112 AAGCTT A^AGCTT
KpnI 23 GGTACC GGTAC^C
MluNI 455 TGGCCA TGG^CCA
MroI 818 TCCGGA T^CCGGA
MscI 455 TGGCCA TGG^CCA
PflMI 200 CCATCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 269 AGGACCT [GA]G^G[AT]CC[CT]
PstI 89 CTGCAG CTGCA^G
SexAI 428 ACCTGGT A^CC[AT]GGT
SfcI 89 CTGCAG C^T[GA][CT]AG
SspBI 434 TGTACA T^GTACA
StyI 555 CCTTGG C^C[AT][AT]GG
TspRI 1218 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 200 CCATCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Asp700I 349 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
822 GAATCTGTTC
AvaII 270 GGACC G^G[AT]CC
927 GGACC
BanI 23 GGTACC G^G[CT][GA]CC
886 GGTGCC
BanII 624 GGGCTC G[GA]GC[CT]^C
1127 GGGCCC
BpmI 288 CTCCAG CTGGAG(16/14)
265 CTGGAG
BsaAI 601 TACGTG [CT]AC^GT[GA]
856 TACGTG
BsrI 866 ACTGG ACTGG(1/-1)
1217 CCAGT
CfoI 529 GCGC GCG^C
921 GCGC
DpnII 42 GATC ^GATC
336 GATC
DsaI 969 CCGCGG C^C[GA][CT]GG
1122 CCGCGG
EaeI 455 TGGCCA [CT]^GGCC[GA]
993 CGGCCG
HgaI 907 GACGC GACGC(5/10)
1008 GACGC
HhaI 529 GCGC GCG^C
921 GCGC
HinP1I 529 GCGC G^CGC
921 GCGC
HphI 240 GGTGA GGTGA(8/7)
1037 TCACC
KspI 969 CCGCGG CCGC^GG
1122 CCGCGG
MboI 42 GATC ^GATC
336 GATC
NdeII 42 GATC ^GATC
336 GATC
NspI 872 ACATGC [GA]CATG^[CT]
1181 GCATGT
PleI 11 GAGTC GAGTC(4/5)
589 GAGTC
SacII 969 CCGCGG CCGC^GG
1122 CCGCGG
Sau3AI 42 GATC ^GATC
336 GATC
TfiI 822 GAATC G^A[AT]TC
1197 GATTC
XmnI 349 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
822 GAATCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI BbvI BcnI BmyI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrBI BssKI BstF5I BstNI
BstUI Cac8I Cfr13I DdeI DraII
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI Ksp632I MaeII MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV RsaI
Sau96I ScrFI SfaNI TaqI TseI
Tsp509I TthHB8I