Restriction map for
pF1KB6587
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BanI BbeI
BbrPI BbvCI BcgI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FseI HaeII HincII HindII HindIII
HpaI KasI KpnI MaeI MaeII
MamI MfeI MluI MroI MseI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StyI SwaI
Tru9I Tsp509I Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcyI 989 GGCGTC G[GA]^CG[CT]C
AflIII 804 ACATGT A^C[GA][CT]GT
AlwNI 142 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 99 GAGCTC G[AT]GC[AT]^C
AviII 959 TGCGCA TGC^GCA
BbsI 172 GAAGAC GAAGAC(2/6)
BclI 1044 TGATCA T^GATCA
BglI 703 GCCCAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 172 GAAGAC GAAGAC(2/6)
BsaHI 989 GGCGTC G[GA]^CG[CT]C
BsaI 226 GAGACC GGTCTC(1/5)
BsiHKAI 99 GAGCTC G[AT]GC[AT]^C
BspLU11I 804 ACATGT A^CATGT
BspMI 56 GCAGGT ACCTGC(4/8)
BsrBI 451 GAGCGG CCGCTC(-3/-3)
BstF5I 900 GGATG GGATG(2/0)
CfoI 960 GCGC GCG^C
FbaI 1044 TGATCA T^GATCA
FokI 900 GGATG GGATG(9/13)
FspI 959 TGCGCA TGC^GCA
HhaI 960 GCGC GCG^C
Hin1I 989 GGCGTC G[GA]^CG[CT]C
HinP1I 960 GCGC G^CGC
KspI 491 CCGCGG CCGC^GG
MspA1I 491 CCGCGG C[AC]G^C[GT]G
PflMI 141 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 99 GAGCTC GAGCT^C
SacII 491 CCGCGG CCGC^GG
SfaNI 1027 GCATC GCATC(5/9)
SmlI 460 CTTGAG C^T[CT][GA]AG
SphI 665 GCATGC GCATG^C
SspI 545 AATATT AAT^ATT
StuI 1054 AGGCCT AGG^CCT
TaqI 613 TCGA T^CGA
TspRI 485 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 613 TCGA T^CGA
Van91I 141 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 908 CCAAGAAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 108 GTAC GT^AC
441 GTAC
AvaI 25 CTCGGG C^[CT]CG[GA]G
196 CCCGAG
BamHI 234 GGATCC G^GATCC
728 GGATCC
BanII 99 GAGCTC G[GA]GC[CT]^C
397 GAGCCC
BbvI 700 GCAGC GCAGC(8/12)
49 GCTGC
BciVI 979 GGATAC GTATCC(6/5)
691 GTATCC
BsmAI 226 GAGAC GTCTC(1/5)
519 GTCTC
BsoBI 25 CTCGGG C^[CT]CG[GA]G
196 CCCGAG
BsrFI 116 GCCGGC [GA]^CCGG[CT]
308 GCCGGC
BstXI 761 CCACCCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
908 CCAAGAAGGTGG
Cfr10I 116 GCCGGC [GA]^CCGG[CT]
308 GCCGGC
DsaI 87 CCGTGG C^C[GA][CT]GG
491 CCGCGG
EcoNI 270 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
969 CCTCAACGAGG
HgaI 610 GCGTC GACGC(5/10)
990 GCGTC
NaeI 116 GCCGGC GCC^GGC
308 GCCGGC
NgoMIV 116 GCCGGC G^CCGGC
308 GCCGGC
NspI 665 GCATGC [GA]CATG^[CT]
804 ACATGT
PpuMI 143 AGGACCT [GA]G^G[AT]CC[CT]
779 GGGACCC
PshAI 567 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
985 GACAGGCGTC
RsaI 108 GTAC GT^AC
441 GTAC
TfiI 533 GATTC G^A[AT]TC
645 GATTC
TseI 49 GCTGC G^C[AT]GC
700 GCAGC
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AluI AlwI AvaII
BalI BcnI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstNI BstUI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI Tsp45I XhoII