Restriction map for
pF1KB6561
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflIII AgeI
AhdI AlwI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BcnI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmFI BsoBI BspDI
BspEI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoRV FauI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MflI MluI MluNI MroI
MscI MslI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SpeI SphI
Sse8387I SspI SwaI Tth111I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AcsI 447 GAATTC [GA]^AATT[CT]
AfaI 396 GTAC GT^AC
AflII 808 CTTAAG C^TTAAG
Alw44I 1093 GTGCAC G^TGCAC
AlwNI 303 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1093 GTGCAC G^TGCAC
ApoI 447 GAATTC [GA]^AATT[CT]
BanII 1105 GAGCCC G[GA]GC[CT]^C
BclI 68 TGATCA T^GATCA
BfaI 749 CTAG C^TAG
BfrI 808 CTTAAG C^TTAAG
BsaAI 938 TACGTA [CT]AC^GT[GA]
BsaWI 1097 ACCGGA [AT]^CCGG[AT]
BsmI 582 GAATGC GAATGC(1/-1)
Bsp1407I 395 TGTACA T^GTACA
BspHI 996 TCATGA T^CATGA
BspMI 321 GCAGGT ACCTGC(4/8)
BsrDI 499 CATTGC GCAATG(2/0)
BsrGI 395 TGTACA T^GTACA
BstEII 621 GGTCACC G^GT[ACGT]ACC
BstPI 621 GGTCACC G^GT[ACGT]ACC
Cac8I 308 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 813 GCGC GCG^C
Cfr13I 1115 GGGCC G^G[ACGT]CC
DpnII 69 GATC ^GATC
DsaI 85 CCATGG C^C[GA][CT]GG
EcoO65I 621 GGTCACC G^GT[ACGT]ACC
EcoRI 447 GAATTC G^AATTC
EcoT14I 85 CCATGG C^C[AT][AT]GG
EcoT22I 584 ATGCAT ATGCA^T
FbaI 68 TGATCA T^GATCA
HapII 1098 CCGG C^CGG
HhaI 813 GCGC GCG^C
HinP1I 813 GCGC G^CGC
HpaII 1098 CCGG C^CGG
MaeI 749 CTAG C^TAG
MaeII 939 ACGT A^CGT
MboI 69 GATC ^GATC
MspI 1098 CCGG C^CGG
NcoI 85 CCATGG C^CATGG
NdeII 69 GATC ^GATC
NsiI 584 ATGCAT ATGCA^T
PflMI 34 CCAGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 996 TCATGA T^CATGA
RsaI 396 GTAC GT^AC
SapI 1045 GAAGAGC GCTCTTC(1/4)
Sau3AI 69 GATC ^GATC
Sau96I 1115 GGGCC G^G[ACGT]CC
SnaBI 938 TACGTA TAC^GTA
SspBI 395 TGTACA T^GTACA
StuI 658 AGGCCT AGG^CCT
StyI 85 CCATGG C^C[AT][AT]GG
Van91I 34 CCAGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 856 CCAACTCCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AspHI 133 GTGCTC G[AT]GC[AT]^C
1093 GTGCAC
BanI 251 GGTGCC G^G[CT][GA]CC
490 GGCACC
BsaI 262 GGTCTC GGTCTC(1/5)
324 GGTCTC
BsiHKAI 133 GTGCTC G[AT]GC[AT]^C
1093 GTGCAC
BsmAI 263 GTCTC GTCTC(1/5)
325 GTCTC
BstXI 138 CCATCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
284 CCATACAGCTGG
EarI 752 GAAGAG CTCTTC(1/4)
1045 GAAGAG
Ksp632I 752 GAAGAG CTCTTC(1/4)
1045 GAAGAG
MseI 431 TTAA T^TAA
809 TTAA
MspA1I 289 CAGCTG C[AC]G^C[GT]G
1078 CAGCTG
PleI 760 GACTC GAGTC(4/5)
335 GAGTC
PstI 319 CTGCAG CTGCA^G
1081 CTGCAG
PvuII 289 CAGCTG CAG^CTG
1078 CAGCTG
SfaNI 519 GCATC GCATC(5/9)
586 GCATC
SmlI 363 CTTGAG C^T[CT][GA]AG
808 CTTAAG
TaqI 196 TCGA T^CGA
414 TCGA
Tru9I 431 TTAA T^TAA
809 TTAA
Tsp509I 448 AATT ^AATT
1011 AATT
TthHB8I 196 TCGA T^CGA
414 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AluI BbsI BbvI BmyI BpmI
BpuAI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI DdeI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeIII MboII MnlI
MvaI MwoI NlaIII NlaIV ScrFI
SfcI TfiI TseI Tsp45I TspRI