Restriction map for
pF1KB6695
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI Aor51HI ApoI AscI AseI
AsnI Asp718I AspI AvaI AviII
AvrII BalI BbeI BbrPI BcgI
BciVI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CelII CfoI ClaI
CpoI DraI DraIII DrdI EaeI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MseI MspA1I MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tth111I Van91I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AluI 426 AGCT AG^CT
Alw44I 410 GTGCAC G^TGCAC
AlwNI 172 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 410 GTGCAC G^TGCAC
Asp700I 370 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1227 GGATCC G^GATCC
BanI 1119 GGTGCC G^G[CT][GA]CC
BbsI 391 GAAGAC GAAGAC(2/6)
BbvCI 427 GCTGAGG CCTCAGC(-5/-2)
BclI 725 TGATCA T^GATCA
BcnI 1123 CCCGG CC^[GC]GG
BglI 1062 GCCCAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 391 GAAGAC GAAGAC(2/6)
BsaI 1177 GAGACC GGTCTC(1/5)
BspMI 1116 GCAGGT ACCTGC(4/8)
BsrFI 1112 ACCGGC [GA]^CCGG[CT]
BstAPI 918 GCAGAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 545 CCACTGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1112 ACCGGC [GA]^CCGG[CT]
DsaI 551 CCATGG C^C[GA][CT]GG
EcoNI 364 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 807 ATGCAT ATGCA^T
FbaI 725 TGATCA T^GATCA
HincII 129 GTCAAC GT[CT]^[GA]AC
HindII 129 GTCAAC GT[CT]^[GA]AC
NciI 1123 CCCGG CC^[GC]GG
NcoI 551 CCATGG C^CATGG
NdeI 146 CATATG CA^TATG
NsiI 807 ATGCAT ATGCA^T
PshAI 220 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 1103 ACCTGGT A^CC[AT]GGT
SfcI 160 CTACAG C^T[GA][CT]AG
StuI 372 AGGCCT AGG^CCT
TaqI 711 TCGA T^CGA
TthHB8I 711 TCGA T^CGA
XbaI 477 TCTAGA T^CTAGA
XmnI 370 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
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AciI 15 CCGC CCGC(-3/-1)
702 CCGC
AhdI 121 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
637 GACCCTGAGTC
AocI 364 CCTGAGG CC^T[ACGT]AGG
523 CCTCAGG
ApaI 273 GGGCCC GGGCC^C
1166 GGGCCC
AspEI 121 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
637 GACCCTGAGTC
BfaI 478 CTAG C^TAG
854 CTAG
BsgI 33 GTGCAG GTGCAG(16/14)
1090 GTGCAG
BsmFI 168 GTCCC GGGAC(10/14)
227 GTCCC
BsrI 250 ACTGG ACTGG(1/-1)
126 CCAGT
BstYI 174 GGATCT [GA]^GATC[CT]
1227 GGATCC
Bsu36I 364 CCTGAGG CC^T[ACGT]AGG
523 CCTCAGG
Eam1105I 121 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
637 GACCCTGAGTC
Eco81I 364 CCTGAGG CC^T[ACGT]AGG
523 CCTCAGG
EcoT14I 551 CCATGG C^C[AT][AT]GG
1162 CCAAGG
HapII 1113 CCGG C^CGG
1124 CCGG
HpaII 1113 CCGG C^CGG
1124 CCGG
MaeI 478 CTAG C^TAG
854 CTAG
MaeIII 501 GTCAC ^GT[ACGT]AC
874 GTCAC
MflI 174 GGATCT [GA]^GATC[CT]
1227 GGATCC
MspI 1113 CCGG C^CGG
1124 CCGG
SmlI 337 CTTGAG C^T[CT][GA]AG
1198 CTTGAG
StyI 551 CCATGG C^C[AT][AT]GG
1162 CCAAGG
Tsp45I 501 GTCAC ^GT[GC]AC
874 GTCAC
XcmI 154 CCACAACTACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
240 CCAGAGGATCACTGG
XhoII 174 GGATCT [GA]^GATC[CT]
1227 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AfaI AlwI AspHI AvaII BanII
BbvI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MboI MboII
MnlI MslI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SphI
TseI Tsp509I TspRI