Restriction map for
pF1KB6691
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BanI BbeI BbrPI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BsrFI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoT22I
FbaI FseI FspI HincII HindII
HpaI KasI KpnI KspI MaeI
MaeII MamI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AlwNI 910 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 429 CCTCAGG CC^T[ACGT]AGG
ApaI 652 GGGCCC GGGCC^C
Asp700I 952 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 160 CTCGGG C^[CT]CG[GA]G
BamHI 99 GGATCC G^GATCC
BbsI 325 GTCTTC GAAGAC(2/6)
BbvCI 756 GCTGAGG CCTCAGC(-5/-2)
BcgI 1013 GCATCAACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 76 CCCGG CC^[GC]GG
BpuAI 325 GTCTTC GAAGAC(2/6)
BsiEI 147 CGACCG CG[GA][CT]^CG
BsmFI 761 GGGAC GGGAC(10/14)
BsoBI 160 CTCGGG C^[CT]CG[GA]G
BspHI 461 TCATGA T^CATGA
BsrBI 93 CCGCTC CCGCTC(-3/-3)
BsrDI 1165 GCAATG GCAATG(2/0)
BssHII 54 GCGCGC G^CGCGC
BstYI 99 GGATCC [GA]^GATC[CT]
Bsu36I 429 CCTCAGG CC^T[ACGT]AGG
DraIII 114 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 88 CTCTTC CTCTTC(1/4)
Eco81I 429 CCTCAGG CC^T[ACGT]AGG
EcoNI 1026 CCTGTTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 743 AGCGCC [GA]GCGC^[CT]
Ksp632I 88 CTCTTC CTCTTC(1/4)
MflI 99 GGATCC [GA]^GATC[CT]
NciI 76 CCCGG CC^[GC]GG
NspI 1151 GCATGT [GA]CATG^[CT]
PstI 1135 CTGCAG CTGCA^G
RcaI 461 TCATGA T^CATGA
SacI 700 GAGCTC GAGCT^C
ScaI 767 AGTACT AGT^ACT
SspI 394 AATATT AAT^ATT
XcmI 707 CCATCTGCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 99 GGATCC [GA]^GATC[CT]
XmnI 952 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 55 CGCG CG^CG
196 CGCG
AcyI 136 GGCGTC G[GA]^CG[CT]C
1264 GACGCC
AlwI 100 GATCC GGATC(4/5)
99 GGATC
AvaII 762 GGACC G^G[AT]CC
1058 GGACC
BalI 238 TGGCCA TGG^CCA
917 TGGCCA
BanII 652 GGGCCC G[GA]GC[CT]^C
700 GAGCTC
BpmI 293 CTCCAG CTGGAG(16/14)
385 CTGGAG
BsaHI 136 GGCGTC G[GA]^CG[CT]C
1264 GACGCC
BseRI 697 GAGGAG GAGGAG(10/8)
739 GAGGAG
BsmAI 680 GAGAC GTCTC(1/5)
1179 GAGAC
BsmI 256 GAATGC GAATGC(1/-1)
1258 GAATGC
BspMI 498 ACCTGC ACCTGC(4/8)
909 GCAGGT
BstUI 55 CGCG CG^CG
196 CGCG
DraII 425 AGGGCCT [GA]G^G[ACGT]CC[CT]
652 GGGCCCC
DsaI 1001 CCATGG C^C[GA][CT]GG
1061 CCATGG
EaeI 238 TGGCCA [CT]^GGCC[GA]
917 TGGCCA
EcoO109I 425 AGGGCCT [GA]G^G[ACGT]CC[CT]
652 GGGCCCC
EcoRV 287 GATATC GAT^ATC
964 GATATC
FauI 66 CCCGC CCCGC(4/6)
252 GCGGG
HapII 77 CCGG C^CGG
747 CCGG
Hin1I 136 GGCGTC G[GA]^CG[CT]C
1264 GACGCC
HindIII 233 AAGCTT A^AGCTT
364 AAGCTT
HpaII 77 CCGG C^CGG
747 CCGG
MluNI 238 TGGCCA TGG^CCA
917 TGGCCA
MscI 238 TGGCCA TGG^CCA
917 TGGCCA
MseI 269 TTAA T^TAA
398 TTAA
MslI 616 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
684 CATCGAGGTG
MspI 77 CCGG C^CGG
747 CCGG
MvnI 55 CGCG CG^CG
196 CGCG
NcoI 1001 CCATGG C^CATGG
1061 CCATGG
SfcI 210 CTGTAG C^T[GA][CT]AG
1135 CTGCAG
Tru9I 269 TTAA T^TAA
398 TTAA
Tsp509I 876 AATT ^AATT
946 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AfaI AluI AspHI BbvI
BmyI BsaJI BsiHKAI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HgaI HhaI HinP1I HinfI HphI
ItaI MaeIII MboI MboII MnlI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I