Restriction map for
pF1KB6673
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AgeI AhdI Alw44I AocI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BamHI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BpuAI BsaBI
BsiWI BsmBI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI Eam1105I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FspI HincII
HindII HindIII HpaI MamI MfeI
MflI MseI MunI NdeI NheI
NotI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SapI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tsp509I Tth111I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 455 GGTACC G^GTACC
AccIII 877 TCCGGA T^CCGGA
AcyI 1213 GGCGCC G[GA]^CG[CT]C
AflIII 512 ACGCGT A^C[GA][CT]GT
Aor51HI 248 AGCGCT AGC^GCT
ApaI 545 GGGCCC GGGCC^C
Asp718I 455 GGTACC G^GTACC
BbeI 1213 GGCGCC GGCGC^C
BfaI 1189 CTAG C^TAG
BsaHI 1213 GGCGCC G[GA]^CG[CT]C
BsaI 10 GGTCTC GGTCTC(1/5)
BsaWI 877 TCCGGA [AT]^CCGG[AT]
BseAI 877 TCCGGA T^CCGGA
BsiEI 363 CGGCCG CG[GA][CT]^CG
BsmI 809 GCATTC GAATGC(1/-1)
BspEI 877 TCCGGA T^CCGGA
BsrDI 805 CATTGC GCAATG(2/0)
BstAPI 675 GCAGCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 938 CCACCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 363 CGGCCG C^GGCCG
EarI 1042 CTCTTC CTCTTC(1/4)
EclXI 363 CGGCCG C^GGCCG
Eco47III 248 AGCGCT AGC^GCT
Eco52I 363 CGGCCG C^GGCCG
EcoNI 1156 CCTTTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 526 GGCCGGCC GGCCGG^CC
Hin1I 1213 GGCGCC G[GA]^CG[CT]C
HphI 292 GGTGA GGTGA(8/7)
KasI 1213 GGCGCC G^GCGCC
KpnI 455 GGTACC GGTAC^C
Ksp632I 1042 CTCTTC CTCTTC(1/4)
KspI 1180 CCGCGG CCGC^GG
MaeI 1189 CTAG C^TAG
MluI 512 ACGCGT A^CGCGT
MroI 877 TCCGGA T^CCGGA
NarI 1213 GGCGCC GG^CGCC
NruI 1080 TCGCGA TCG^CGA
PflMI 771 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 943 CAGCTG CAG^CTG
SacII 1180 CCGCGG CCGC^GG
SexAI 590 ACCTGGT A^CC[AT]GGT
SfoI 1213 GGCGCC GGC^GCC
SmaI 314 CCCGGG CCC^GGG
SmlI 184 CTCAAG C^T[CT][GA]AG
TfiI 669 GATTC G^A[AT]TC
Tsp45I 1013 GTGAC ^GT[GC]AC
Van91I 771 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 314 CCCGGG C^CCGGG
XmaI 314 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwI 1084 GATCC GGATC(4/5)
483 GGATC
AlwNI 79 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
265 CAGCTTCTG
AspHI 112 GAGCAC G[AT]GC[AT]^C
130 GAGCAC
BalI 416 TGGCCA TGG^CCA
970 TGGCCA
BglI 313 GCCCGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1118 GCCGAGAGGGC
BlpI 262 GCTCAGC GC^T[ACGT]AGC
1221 GCTGAGC
Bpu1102I 262 GCTCAGC GC^T[ACGT]AGC
1221 GCTGAGC
BseRI 619 GAGGAG GAGGAG(10/8)
1201 GAGGAG
BsiHKAI 112 GAGCAC G[AT]GC[AT]^C
130 GAGCAC
BspMI 210 GCAGGT ACCTGC(4/8)
286 GCAGGT
BsrFI 527 GCCGGC [GA]^CCGG[CT]
784 GCCGGC
CelII 262 GCTCAGC GC^T[ACGT]AGC
1221 GCTGAGC
Cfr10I 527 GCCGGC [GA]^CCGG[CT]
784 GCCGGC
DraIII 496 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
974 CACACAGTG
HaeII 248 AGCGCT [GA]GCGC^[CT]
1213 GGCGCC
MaeIII 1007 GTTAC ^GT[ACGT]AC
1013 GTGAC
MluNI 416 TGGCCA TGG^CCA
970 TGGCCA
MscI 416 TGGCCA TGG^CCA
970 TGGCCA
MslI 414 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
564 CATGGCGGTG
MspA1I 943 CAGCTG C[AC]G^C[GT]G
1180 CCGCGG
NaeI 527 GCCGGC GCC^GGC
784 GCCGGC
NcoI 413 CCATGG C^CATGG
563 CCATGG
NgoMIV 527 GCCGGC G^CCGGC
784 GCCGGC
PleI 48 GACTC GAGTC(4/5)
354 GACTC
StuI 56 AGGCCT AGG^CCT
900 AGGCCT
TaqI 245 TCGA T^CGA
834 TCGA
TthHB8I 245 TCGA T^CGA
834 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
AvaII BanI BanII BbrPI BbvI
BcnI BmyI BpmI BsaAI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII ItaI MaeII
MboI MboII MnlI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PmaCI PmlI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TseI TspRI