Restriction map for
pF1KB6671
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Aor51HI ApaI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BanII BbvCI BciVI BclI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII Bst1107I BstBI BstZ17I
CelII ClaI CpoI DraI DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I MaeI MfeI
MluI MroI MseI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfiI SfuI SnaBI SpeI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tsp509I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 13 GTCTAC GT^[AC][GT]AC
Alw44I 844 GTGCAC G^TGCAC
AlwNI 1164 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 904 CCTGAGG CC^T[ACGT]AGG
ApaLI 844 GTGCAC G^TGCAC
Asp700I 186 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 482 GGTCC G^G[AT]CC
BalI 629 TGGCCA TGG^CCA
BbeI 883 GGCGCC GGCGC^C
BbsI 1101 GAAGAC GAAGAC(2/6)
BglII 188 AGATCT A^GATCT
BpuAI 1101 GAAGAC GAAGAC(2/6)
BsaBI 1215 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspLU11I 749 ACATGT A^CATGT
BsrDI 1131 CATTGC GCAATG(2/0)
BsrFI 1042 ACCGGC [GA]^CCGG[CT]
BssSI 493 CTCGTG CACGAG(-5/-1)
BstAPI 342 GCACCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 975 GGTGACC G^GT[ACGT]ACC
BstPI 975 GGTGACC G^GT[ACGT]ACC
BstXI 113 CCACCACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 904 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1042 ACCGGC [GA]^CCGG[CT]
Eco81I 904 CCTGAGG CC^T[ACGT]AGG
EcoNI 577 CCTCTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 975 GGTGACC G^GT[ACGT]ACC
HgaI 11 GCGTC GACGC(5/10)
KasI 883 GGCGCC G^GCGCC
KspI 134 CCGCGG CCGC^GG
MamI 1215 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 629 TGGCCA TGG^CCA
MscI 629 TGGCCA TGG^CCA
MspA1I 134 CCGCGG C[AC]G^C[GT]G
NarI 883 GGCGCC GG^CGCC
NcoI 1148 CCATGG C^CATGG
SacII 134 CCGCGG CCGC^GG
SfoI 883 GGCGCC GGC^GCC
SgrAI 1041 CACCGGCG C[GA]^CCGG[CT]G
SmaI 1014 CCCGGG CCC^GGG
SmlI 253 CTCAAG C^T[CT][GA]AG
SphI 1115 GCATGC GCATG^C
StuI 779 AGGCCT AGG^CCT
XcmI 953 CCATGTTGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1014 CCCGGG C^CCGGG
XmaI 1014 CCCGGG C^CCGGG
XmnI 186 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 70 CGCG CG^CG
135 CGCG
AcyI 10 GGCGTC G[GA]^CG[CT]C
883 GGCGCC
AflIII 318 ACACGT A^C[GA][CT]GT
749 ACATGT
AvaI 224 CTCGGG C^[CT]CG[GA]G
1014 CCCGGG
BanI 685 GGTGCC G^G[CT][GA]CC
883 GGCGCC
BbrPI 217 CACGTG CAC^GTG
970 CACGTG
BcgI 201 CGACACCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
216 GCACGTGCCTCG
BpmI 154 CTGGAG CTGGAG(16/14)
160 CTGGAG
BsaAI 217 CACGTG [CT]AC^GT[GA]
970 CACGTG
BsaHI 10 GGCGTC G[GA]^CG[CT]C
883 GGCGCC
BsaI 726 GGTCTC GGTCTC(1/5)
795 GGTCTC
BseRI 139 GAGGAG GAGGAG(10/8)
1240 GAGGAG
BsmAI 727 GTCTC GTCTC(1/5)
796 GTCTC
BsoBI 224 CTCGGG C^[CT]CG[GA]G
1014 CCCGGG
BspMI 338 ACCTGC ACCTGC(4/8)
792 GCAGGT
BsrBI 3 GAGCGG CCGCTC(-3/-3)
233 GAGCGG
BstUI 70 CGCG CG^CG
135 CGCG
BstYI 188 AGATCT [GA]^GATC[CT]
1220 GGATCT
DraII 908 AGGGCCT [GA]G^G[ACGT]CC[CT]
935 AGGGCCT
DraIII 343 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
970 CACGTGGTG
EcoO109I 908 AGGGCCT [GA]G^G[ACGT]CC[CT]
935 AGGGCCT
HaeII 36 GGCGCT [GA]GCGC^[CT]
883 GGCGCC
Hin1I 10 GGCGTC G[GA]^CG[CT]C
883 GGCGCC
HinfI 802 GACTC G^A[ACGT]TC
893 GACTC
MflI 188 AGATCT [GA]^GATC[CT]
1220 GGATCT
MvnI 70 CGCG CG^CG
135 CGCG
PflMI 621 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
731 CCAAGTCCTGG
PleI 802 GACTC GAGTC(4/5)
893 GACTC
PmaCI 217 CACGTG CAC^GTG
970 CACGTG
PmlI 217 CACGTG CAC^GTG
970 CACGTG
Van91I 621 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
731 CCAAGTCCTGG
XhoII 188 AGATCT [GA]^GATC[CT]
1220 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BbvI BcnI BmyI BsaJI BsgI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TseI Tsp45I TspRI TthHB8I