Restriction map for
pF1KB6648
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsgI BsiWI BsmBI BsmFI BspDI
BspMI BsrBI BsrDI BsrFI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI Eam1105I Eco47III Eco81I
EcoNI EcoO65I EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HpaI
HphI KasI KpnI KspI MfeI
MluI MluNI MscI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
StuI SwaI Tsp45I Tth111I Van91I
XbaI XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 74 TCCGGA T^CCGGA
AflIII 545 ACATGT A^C[GA][CT]GT
AseI 633 ATTAAT AT^TAAT
AsnI 633 ATTAAT AT^TAAT
Asp700I 787 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 953 GAGCTC G[AT]GC[AT]^C
AvaI 8 CCCGGG C^[CT]CG[GA]G
BbvCI 13 GCTGAGG CCTCAGC(-5/-2)
BsaBI 766 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 74 TCCGGA T^CCGGA
BseRI 1185 GAGGAG GAGGAG(10/8)
BsiEI 57 CGGCCG CG[GA][CT]^CG
BsiHKAI 953 GAGCTC G[AT]GC[AT]^C
BsmI 376 GAATGC GAATGC(1/-1)
BsoBI 8 CCCGGG C^[CT]CG[GA]G
BspEI 74 TCCGGA T^CCGGA
BspHI 1181 TCATGA T^CATGA
BspLU11I 545 ACATGT A^CATGT
BsrI 1032 ACTGG ACTGG(1/-1)
BssSI 507 CACGAG CACGAG(-5/-1)
CfoI 1332 GCGC GCG^C
DraII 1232 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 57 CGGCCG [CT]^GGCC[GA]
EagI 57 CGGCCG C^GGCCG
EclXI 57 CGGCCG C^GGCCG
Eco52I 57 CGGCCG C^GGCCG
EcoO109I 1232 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 592 GATATC GAT^ATC
EcoT14I 305 CCAAGG C^C[AT][AT]GG
FauI 1266 GCGGG CCCGC(4/6)
HhaI 1332 GCGC GCG^C
HinP1I 1332 GCGC G^CGC
HincII 357 GTTGAC GT[CT]^[GA]AC
HindII 357 GTTGAC GT[CT]^[GA]AC
HindIII 466 AAGCTT A^AGCTT
MaeII 461 ACGT A^CGT
MamI 766 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 74 TCCGGA T^CCGGA
NheI 483 GCTAGC G^CTAGC
PpuMI 1232 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 633 ATTAAT AT^TAAT
PvuII 1261 CAGCTG CAG^CTG
RcaI 1181 TCATGA T^CATGA
SacI 953 GAGCTC GAGCT^C
SfcI 1062 CTACAG C^T[GA][CT]AG
SmaI 8 CCCGGG CCC^GGG
SphI 1328 GCATGC GCATG^C
StyI 305 CCAAGG C^C[AT][AT]GG
XcmI 434 CCAATGTTTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 8 CCCGGG C^CCGGG
XmaI 8 CCCGGG C^CCGGG
XmnI 787 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AciI 60 CCGC CCGC(-3/-1)
1266 GCGG
AfaI 33 GTAC GT^AC
246 GTAC
AlwNI 134 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1318 CAGATCCTG
AvaII 230 GGACC G^G[AT]CC
1233 GGACC
BanII 953 GAGCTC G[GA]GC[CT]^C
1268 GGGCTC
BcnI 8 CCCGG CC^[GC]GG
9 CCGGG
BmyI 953 GAGCTC G[AGT]GC[ACT]^C
1268 GGGCTC
BsaWI 74 TCCGGA [AT]^CCGG[AT]
227 ACCGGA
Bsp1286I 953 GAGCTC G[AGT]GC[ACT]^C
1268 GGGCTC
Bsp1407I 32 TGTACA T^GTACA
245 TGTACA
BsrGI 32 TGTACA T^GTACA
245 TGTACA
BstNI 444 CCTGG CC^[AT]GG
1323 CCTGG
EcoRI 704 GAATTC G^AATTC
1194 GAATTC
EcoRII 444 CCTGG ^CC[AT]GG
1323 CCTGG
HaeIII 58 GGCC GG^CC
366 GGCC
MslI 249 CATTGTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1153 CATTTCAGTG
MspA1I 60 CCGCTG C[AC]G^C[GT]G
1261 CAGCTG
MvaI 444 CCTGG CC^[AT]GG
1323 CCTGG
MwoI 50 GCTCCTACGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
955 GCTCTCAAAGC
NciI 8 CCCGG CC^[GC]GG
9 CCGGG
RsaI 33 GTAC GT^AC
246 GTAC
SfaNI 150 GATGC GCATC(5/9)
414 GCATC
SspBI 32 TGTACA T^GTACA
245 TGTACA
SspI 260 AATATT AAT^ATT
492 AATATT
TaqI 342 TCGA T^CGA
774 TCGA
TfiI 564 GAATC G^A[AT]TC
892 GATTC
TspRI 242 CACTG CA[GC]TG[ACGT][ACGT]^
1158 CAGTG
TthHB8I 342 TCGA T^CGA
774 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbvI
BfaI BsaJI BsiYI BslI BsmAI
BssKI BstF5I BstYI Cac8I Cfr13I
DdeI DpnII EarI Fnu4HI FokI
HapII HinfI HpaII ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MspI NdeII NlaIII
NlaIV NspI PleI Sau3AI Sau96I
ScrFI SmlI Tru9I TseI Tsp509I
XhoII