Restriction map for
pF1KB6631
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AfaI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BanI BbeI
BcgI BclI BfaI BfrI BglI
BlnI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflFI PinAI PmeI
PshAI PshBI PvuI RcaI RsaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AclI 1048 AACGTT AA^CGTT
AlwI 1124 GGATC GGATC(4/5)
ApaI 863 GGGCCC GGGCC^C
Asp700I 1165 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 268 GAGCTC G[AT]GC[AT]^C
AvaI 83 CTCGAG C^[CT]CG[GA]G
BalI 114 TGGCCA TGG^CCA
BbrPI 975 CACGTG CAC^GTG
BbvCI 889 GCTGAGG CCTCAGC(-5/-2)
BciVI 88 GGATAC GTATCC(6/5)
BlpI 98 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 98 GCTTAGC GC^T[ACGT]AGC
BsaAI 975 CACGTG [CT]AC^GT[GA]
BsaBI 958 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 268 GAGCTC G[AT]GC[AT]^C
BsoBI 83 CTCGAG C^[CT]CG[GA]G
BsrI 827 ACTGG ACTGG(1/-1)
Cac8I 847 GCCTGC GC[ACGT]^[ACGT]GC
CelII 98 GCTTAGC GC^T[ACGT]AGC
CfoI 1067 GCGC GCG^C
DsaI 117 CCATGG C^C[GA][CT]GG
EaeI 114 TGGCCA [CT]^GGCC[GA]
HgaI 223 GACGC GACGC(5/10)
HhaI 1067 GCGC GCG^C
HinP1I 1067 GCGC G^CGC
MamI 958 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 114 TGGCCA TGG^CCA
MscI 114 TGGCCA TGG^CCA
MseI 251 TTAA T^TAA
MslI 939 CACCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 117 CCATGG C^CATGG
PaeR7I 83 CTCGAG C^TCGAG
PflMI 1115 CCAAATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 975 CACGTG CAC^GTG
PmlI 975 CACGTG CAC^GTG
PpuMI 185 AGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 1048 AACGTT AA^CGTT
SacI 268 GAGCTC GAGCT^C
SfaNI 495 GATGC GCATC(5/9)
SmlI 83 CTCGAG C^T[CT][GA]AG
StuI 63 AGGCCT AGG^CCT
TaqI 84 TCGA T^CGA
TfiI 71 GATTC G^A[AT]TC
Tru9I 251 TTAA T^TAA
Tsp45I 5 GTGAC ^GT[GC]AC
TthHB8I 84 TCGA T^CGA
Van91I 1115 CCAAATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 83 CTCGAG C^TCGAG
XmnI 1165 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccII 225 CGCG CG^CG
1068 CGCG
AcsI 1072 GAATTT [GA]^AATT[CT]
1117 AAATTC
ApoI 1072 GAATTT [GA]^AATT[CT]
1117 AAATTC
AvaII 121 GGTCC G^G[AT]CC
186 GGTCC
BbsI 1060 GAAGAC GAAGAC(2/6)
918 GTCTTC
BpuAI 1060 GAAGAC GAAGAC(2/6)
918 GTCTTC
BsrDI 981 GCAATG GCAATG(2/0)
1082 GCAATG
BstF5I 944 GGATG GGATG(2/0)
1158 GGATG
BstUI 225 CGCG CG^CG
1068 CGCG
DraII 185 AGGTCCC [GA]G^G[ACGT]CC[CT]
862 GGGGCCC
EcoO109I 185 AGGTCCC [GA]G^G[ACGT]CC[CT]
862 GGGGCCC
FokI 944 GGATG GGATG(9/13)
1158 GGATG
MaeII 976 ACGT A^CGT
1049 ACGT
MvnI 225 CGCG CG^CG
1068 CGCG
NlaIII 118 CATG CATG^
807 CATG
Tsp509I 1073 AATT ^AATT
1118 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AlwNI BanII BbvI
BcnI BglII BmyI BpmI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BssKI BstNI BstYI Cfr13I DdeI
DpnII EcoNI EcoRII EcoT14I Fnu4HI
HaeIII HapII HinfI HpaII HphI
ItaI MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIV PleI PstI
PvuII Sau3AI Sau96I ScrFI SfcI
StyI TseI TspRI XhoII