Restriction map for
pF1KB6628
##################################
# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HindIII
HpaI KasI KpnI KspI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SpeI SphI Sse8387I StuI
SwaI Tth111I XbaI XcmI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AlwNI 964 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 376 CCTGAGG CC^T[ACGT]AGG
AvaI 434 CTCGAG C^[CT]CG[GA]G
BbsI 724 GAAGAC GAAGAC(2/6)
BcgI 155 GCAGCACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 104 CTAG C^TAG
BglII 438 AGATCT A^GATCT
BpuAI 724 GAAGAC GAAGAC(2/6)
BsaBI 190 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 142 GAGACC GGTCTC(1/5)
BseRI 379 GAGGAG GAGGAG(10/8)
BsgI 706 CTGCAC GTGCAG(16/14)
BsoBI 434 CTCGAG C^[CT]CG[GA]G
Bsp1407I 609 TGTACA T^GTACA
BsrBI 801 CCGCTC CCGCTC(-3/-3)
BsrGI 609 TGTACA T^GTACA
BstAPI 582 GCAGCGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 376 CCTGAGG CC^T[ACGT]AGG
DsaI 647 CCATGG C^C[GA][CT]GG
EarI 61 GAAGAG CTCTTC(1/4)
Eco81I 376 CCTGAGG CC^T[ACGT]AGG
HaeII 1024 GGCGCT [GA]GCGC^[CT]
HphI 249 TCACC GGTGA(8/7)
Ksp632I 61 GAAGAG CTCTTC(1/4)
MaeI 104 CTAG C^TAG
MamI 190 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 89 TTAA T^TAA
MspA1I 583 CAGCGG C[AC]G^C[GT]G
NcoI 647 CCATGG C^CATGG
NspI 728 ACATGC [GA]CATG^[CT]
PaeR7I 434 CTCGAG C^TCGAG
PflMI 963 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 301 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfcI 461 CTGTAG C^T[GA][CT]AG
SmlI 434 CTCGAG C^T[CT][GA]AG
SnaBI 482 TACGTA TAC^GTA
SspBI 609 TGTACA T^GTACA
SspI 769 AATATT AAT^ATT
Tru9I 89 TTAA T^TAA
Van91I 963 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 434 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AccI 272 GTCGAC GT^[AC][GT]AC
890 GTCGAC
AccII 290 CGCG CG^CG
475 CGCG
AcsI 425 GAATTC [GA]^AATT[CT]
535 GAATTC
AfaI 481 GTAC GT^AC
610 GTAC
ApaI 854 GGGCCC GGGCC^C
1121 GGGCCC
ApoI 425 GAATTC [GA]^AATT[CT]
535 GAATTC
BcnI 735 CCGGG CC^[GC]GG
848 CCCGG
BglI 847 GCCCGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1075 GCCGCCCAGGC
BpmI 1191 CTCCAG CTGGAG(16/14)
442 CTGGAG
BsaAI 482 TACGTA [CT]AC^GT[GA]
529 TACGTG
BsmAI 142 GAGAC GTCTC(1/5)
169 GAGAC
BsmFI 299 GGGAC GGGAC(10/14)
959 GGGAC
BspMI 659 ACCTGC ACCTGC(4/8)
985 GCAGGT
BstF5I 66 GGATG GGATG(2/0)
927 GGATG
BstUI 290 CGCG CG^CG
475 CGCG
BstYI 438 AGATCT [GA]^GATC[CT]
972 GGATCT
CfoI 687 GCGC GCG^C
1025 GCGC
EcoNI 660 CCTGCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
777 CCTGATGAAGG
EcoRI 425 GAATTC G^AATTC
535 GAATTC
EcoT14I 647 CCATGG C^C[AT][AT]GG
1115 CCTTGG
FauI 392 CCCGC CCCGC(4/6)
852 GCGGG
FokI 66 GGATG GGATG(9/13)
927 GGATG
HapII 735 CCGG C^CGG
849 CCGG
HhaI 687 GCGC GCG^C
1025 GCGC
HinP1I 687 GCGC G^CGC
1025 GCGC
HincII 272 GTCGAC GT[CT]^[GA]AC
890 GTCGAC
HindII 272 GTCGAC GT[CT]^[GA]AC
890 GTCGAC
HpaII 735 CCGG C^CGG
849 CCGG
MaeIII 248 GTCAC ^GT[ACGT]AC
913 GTGAC
MboII 61 GAAGA GAAGA(8/7)
724 GAAGA
MflI 438 AGATCT [GA]^GATC[CT]
972 GGATCT
MslI 277 CATGGTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
304 CATCGTCGTG
MspI 735 CCGG C^CGG
849 CCGG
MvnI 290 CGCG CG^CG
475 CGCG
NciI 735 CCGGG CC^[GC]GG
848 CCCGG
NruI 289 TCGCGA TCG^CGA
474 TCGCGA
RsaI 481 GTAC GT^AC
610 GTAC
SalI 272 GTCGAC G^TCGAC
890 GTCGAC
StyI 647 CCATGG C^C[AT][AT]GG
1115 CCTTGG
Tsp45I 248 GTCAC ^GT[GC]AC
913 GTGAC
XhoII 438 AGATCT [GA]^GATC[CT]
972 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AlwI AvaII BalI
BanII BbvI BmyI BsaJI BsiYI
BslI Bsp1286I BsrDI BsrI BssKI
BstNI Cac8I Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII Fnu4HI
HaeIII HinfI ItaI MaeII MboI
MluNI MnlI MscI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
TseI Tsp509I TspRI TthHB8I