Restriction map for
pF1KB6612
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BclI BcnI BfrI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HpaI KasI KpnI
MfeI MluI MslI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccIII 1008 TCCGGA T^CCGGA
AcsI 741 AAATTC [GA]^AATT[CT]
ApoI 741 AAATTC [GA]^AATT[CT]
Asp700I 805 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1215 TGGCCA TGG^CCA
BbsI 910 GAAGAC GAAGAC(2/6)
BglII 935 AGATCT A^GATCT
BpmI 586 CTGGAG CTGGAG(16/14)
BpuAI 910 GAAGAC GAAGAC(2/6)
BsaBI 36 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1225 GGTCTC GGTCTC(1/5)
BsaWI 1008 TCCGGA [AT]^CCGG[AT]
BseAI 1008 TCCGGA T^CCGGA
BsgI 578 CTGCAC GTGCAG(16/14)
BspEI 1008 TCCGGA T^CCGGA
BspMI 298 ACCTGC ACCTGC(4/8)
BsrI 860 CCAGT ACTGG(1/-1)
BstAPI 220 GCAACAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1093 TTCGAA TT^CGAA
BstEII 1049 GGTCACC G^GT[ACGT]ACC
BstF5I 473 GGATG GGATG(2/0)
BstPI 1049 GGTCACC G^GT[ACGT]ACC
CfoI 419 GCGC GCG^C
DraIII 45 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1215 TGGCCA [CT]^GGCC[GA]
EcoO65I 1049 GGTCACC G^GT[ACGT]ACC
EcoT14I 735 CCTTGG C^C[AT][AT]GG
FokI 473 GGATG GGATG(9/13)
HapII 1009 CCGG C^CGG
HhaI 419 GCGC GCG^C
HinP1I 419 GCGC G^CGC
HindIII 787 AAGCTT A^AGCTT
HpaII 1009 CCGG C^CGG
KspI 8 CCGCGG CCGC^GG
MaeII 173 ACGT A^CGT
MamI 36 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1215 TGGCCA TGG^CCA
MroI 1008 TCCGGA T^CCGGA
MscI 1215 TGGCCA TGG^CCA
MspI 1009 CCGG C^CGG
NspI 302 GCATGC [GA]CATG^[CT]
NspV 1093 TTCGAA TT^CGAA
PflMI 462 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1037 GGGACCC [GA]G^G[AT]CC[CT]
PstI 572 CTGCAG CTGCA^G
PvuII 575 CAGCTG CAG^CTG
SacI 634 GAGCTC GAGCT^C
SacII 8 CCGCGG CCGC^GG
ScaI 289 AGTACT AGT^ACT
SfcI 572 CTGCAG C^T[GA][CT]AG
SfuI 1093 TTCGAA TT^CGAA
SmlI 496 CTCAAG C^T[CT][GA]AG
SphI 302 GCATGC GCATG^C
StyI 735 CCTTGG C^C[AT][AT]GG
Tsp509I 742 AATT ^AATT
Van91I 462 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 805 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 9 CGCG CG^CG
137 CGCG
AfaI 290 GTAC GT^AC
948 GTAC
AlwI 751 GATCC GGATC(4/5)
339 GGATC
AlwNI 463 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1148 CAGAGCCTG
AvaI 412 CCCGAG C^[CT]CG[GA]G
902 CTCGGG
BciVI 426 GGATAC GTATCC(6/5)
731 GTATCC
BfaI 113 CTAG C^TAG
212 CTAG
BglI 17 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
596 GCCACAGTGGC
BsmAI 900 GTCTC GTCTC(1/5)
1226 GTCTC
BsoBI 412 CCCGAG C^[CT]CG[GA]G
902 CTCGGG
BstUI 9 CGCG CG^CG
137 CGCG
BstYI 339 GGATCT [GA]^GATC[CT]
935 AGATCT
DdeI 665 CTGAG C^T[ACGT]AG
939 CTCAG
DsaI 8 CCGCGG C^C[GA][CT]GG
1221 CCACGG
FauI 7 CCCGC CCCGC(4/6)
10 GCGGG
MaeI 113 CTAG C^TAG
212 CTAG
MflI 339 GGATCT [GA]^GATC[CT]
935 AGATCT
MvnI 9 CGCG CG^CG
137 CGCG
RsaI 290 GTAC GT^AC
948 GTAC
SfaNI 36 GATGC GCATC(5/9)
226 GATGC
TaqI 628 TCGA T^CGA
1094 TCGA
TfiI 349 GATTC G^A[AT]TC
404 GAATC
TthHB8I 628 TCGA T^CGA
1094 TCGA
XhoII 339 GGATCT [GA]^GATC[CT]
935 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI ApaI AspHI AvaII
BanII BbvI BmyI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BssKI BstNI Cac8I Cfr13I DpnII
DraII EarI EcoO109I EcoRII Fnu4HI
HaeIII HinfI HphI ItaI Ksp632I
MaeIII MboI MboII MnlI MseI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
Tru9I TseI Tsp45I TspRI