Restriction map for
pF1KB6607
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI AlwI Aor51HI ApaI
AscI Asp718I AspEI AspI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstYI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MspI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Alw44I 545 GTGCAC G^TGCAC
AocI 1090 CCTCAGG CC^T[ACGT]AGG
ApaLI 545 GTGCAC G^TGCAC
AseI 135 ATTAAT AT^TAAT
AsnI 135 ATTAAT AT^TAAT
Asp700I 988 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 545 GTGCAC G[AT]GC[AT]^C
AvaI 55 CCCGAG C^[CT]CG[GA]G
BanII 743 GGGCTC G[GA]GC[CT]^C
BcgI 912 GCAGATAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsiHKAI 545 GTGCAC G[AT]GC[AT]^C
BsmBI 61 GAGACG CGTCTC(1/5)
BsmFI 340 GTCCC GGGAC(10/14)
BsoBI 55 CCCGAG C^[CT]CG[GA]G
BstXI 311 CCACAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1090 CCTCAGG CC^T[ACGT]AGG
Cfr13I 351 GGCCC G^G[ACGT]CC
DpnII 588 GATC ^GATC
DrdI 555 GACGGGTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1090 CCTCAGG CC^T[ACGT]AGG
EcoT14I 347 CCTTGG C^C[AT][AT]GG
HaeIII 351 GGCC GG^CC
HincII 715 GTTGAC GT[CT]^[GA]AC
HindII 715 GTTGAC GT[CT]^[GA]AC
MboI 588 GATC ^GATC
MspA1I 770 CAGCTG C[AC]G^C[GT]G
NdeI 1066 CATATG CA^TATG
NdeII 588 GATC ^GATC
NlaIII 591 CATG CATG^
PshBI 135 ATTAAT AT^TAAT
PvuII 770 CAGCTG CAG^CTG
Sau3AI 588 GATC ^GATC
Sau96I 351 GGCCC G^G[ACGT]CC
ScaI 736 AGTACT AGT^ACT
SmlI 725 CTCAAG C^T[CT][GA]AG
StyI 347 CCTTGG C^C[AT][AT]GG
TaqI 921 TCGA T^CGA
TthHB8I 921 TCGA T^CGA
XcmI 308 CCACCACAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 988 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 829 GAATTT [GA]^AATT[CT]
1182 GAATTT
AfaI 497 GTAC GT^AC
737 GTAC
ApoI 829 GAATTT [GA]^AATT[CT]
1182 GAATTT
BbsI 15 GAAGAC GAAGAC(2/6)
753 GTCTTC
BmyI 545 GTGCAC G[AGT]GC[ACT]^C
743 GGGCTC
BpuAI 15 GAAGAC GAAGAC(2/6)
753 GTCTTC
BsgI 410 CTGCAC GTGCAG(16/14)
422 GTGCAG
Bsp1286I 545 GTGCAC G[AGT]GC[ACT]^C
743 GGGCTC
BssKI 688 CCTGG ^CC[ACGT]GG
960 CCAGG
BstAPI 538 GCAACGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
760 GCAGTGGCTGC
BstEII 6 GGTCACC G^GT[ACGT]ACC
219 GGTGACC
BstF5I 371 CATCC GGATG(2/0)
820 CATCC
BstNI 688 CCTGG CC^[AT]GG
960 CCAGG
BstPI 6 GGTCACC G^GT[ACGT]ACC
219 GGTGACC
EcoO65I 6 GGTCACC G^GT[ACGT]ACC
219 GGTGACC
EcoRII 688 CCTGG ^CC[AT]GG
960 CCAGG
FokI 371 CATCC GGATG(9/13)
820 CATCC
MslI 312 CACAACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
591 CATGTCAATG
MvaI 688 CCTGG CC^[AT]GG
960 CCAGG
RsaI 497 GTAC GT^AC
737 GTAC
ScrFI 688 CCTGG CC^[ACGT]GG
960 CCAGG
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AluI AlwNI BbvI BfaI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BspMI BsrI Cac8I DdeI Fnu4HI
HinfI HphI ItaI MaeI MaeIII
MboII MnlI MseI MwoI NlaIV
PstI SfaNI SfcI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI