Restriction map for
pF1KB6302
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AvaII AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AciI 451 GCGG CCGC(-3/-1)
AspHI 183 GAGCAC G[AT]GC[AT]^C
AspI 721 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcnI 234 CCGGG CC^[GC]GG
BglII 545 AGATCT A^GATCT
BmyI 183 GAGCAC G[AGT]GC[ACT]^C
BpmI 21 CTGGAG CTGGAG(16/14)
BsaJI 195 CCAGGG C^C[ACGT][ACGT]GG
BsgI 413 GTGCAG GTGCAG(16/14)
BsiHKAI 183 GAGCAC G[AT]GC[AT]^C
Bsp1286I 183 GAGCAC G[AGT]GC[ACT]^C
Cfr13I 192 GGGCC G^G[ACGT]CC
EarI 147 GAAGAG CTCTTC(1/4)
HapII 234 CCGG C^CGG
HgaI 408 GCGTC GACGC(5/10)
HindIII 174 AAGCTT A^AGCTT
HpaII 234 CCGG C^CGG
Ksp632I 147 GAAGAG CTCTTC(1/4)
MaeII 583 ACGT A^CGT
MslI 553 CACCTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 234 CCGG C^CGG
NciI 234 CCGGG CC^[GC]GG
NlaIII 354 CATG CATG^
PflFI 721 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 630 CCAGCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 755 CTGCAG CTGCA^G
Sau96I 192 GGGCC G^G[ACGT]CC
SexAI 701 ACCTGGT A^CC[AT]GGT
SfaNI 589 GCATC GCATC(5/9)
StuI 264 AGGCCT AGG^CCT
TfiI 67 GAATC G^A[AT]TC
Tth111I 721 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 630 CCAGCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AlwI 350 GGATC GGATC(4/5)
690 GGATC
AlwNI 15 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
631 CAGCGACTG
BfaI 142 CTAG C^TAG
448 CTAG
BseRI 475 GAGGAG GAGGAG(10/8)
793 GAGGAG
BstF5I 777 GGATG GGATG(2/0)
810 GGATG
BstXI 249 CCACACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
650 CCAAAGGAATGG
BstYI 545 AGATCT [GA]^GATC[CT]
690 GGATCT
FokI 777 GGATG GGATG(9/13)
810 GGATG
MaeI 142 CTAG C^TAG
448 CTAG
MaeIII 580 GTCAC ^GT[ACGT]AC
719 GTGAC
MflI 545 AGATCT [GA]^GATC[CT]
690 GGATCT
MseI 167 TTAA T^TAA
461 TTAA
MspA1I 15 CAGCTG C[AC]G^C[GT]G
784 CAGCTG
MwoI 176 GCTTTGAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
185 GCACTGTGGGC
PvuII 15 CAGCTG CAG^CTG
784 CAGCTG
SfcI 12 CTACAG C^T[GA][CT]AG
755 CTGCAG
TaqI 438 TCGA T^CGA
744 TCGA
Tru9I 167 TTAA T^TAA
461 TTAA
Tsp45I 580 GTCAC ^GT[GC]AC
719 GTGAC
Tsp509I 346 AATT ^AATT
457 AATT
TthHB8I 438 TCGA T^CGA
744 TCGA
XhoII 545 AGATCT [GA]^GATC[CT]
690 GGATCT
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AluI BbvI BsiYI BslI BsmAI
BsrI BssKI BstNI Cac8I DdeI
DpnII EcoRII Fnu4HI HaeIII HinfI
HphI ItaI MboI MboII MnlI
MvaI NdeII NlaIV PleI Sau3AI
ScrFI TseI TspRI