Restriction map for
pF1KB6123
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI AlwNI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AvaI AviII
AvrII BamHI BbrPI BclI BcnI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaWI BseAI BsgI BsiEI BsiWI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HpaI KpnI KspI MaeIII
MluI MroI MslI MvnI NciI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcyI 79 GGCGCC G[GA]^CG[CT]C
AfaI 1507 GTAC GT^AC
AocI 970 CCTGAGG CC^T[ACGT]AGG
BalI 628 TGGCCA TGG^CCA
BanI 79 GGCGCC G^G[CT][GA]CC
BbeI 79 GGCGCC GGCGC^C
BbsI 1493 GTCTTC GAAGAC(2/6)
BbvCI 1420 GCTGAGG CCTCAGC(-5/-2)
BcgI 722 CGAATTCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1358 GTATCC GTATCC(6/5)
BpmI 359 CTGGAG CTGGAG(16/14)
BpuAI 1493 GTCTTC GAAGAC(2/6)
BsaHI 79 GGCGCC G[GA]^CG[CT]C
BsaI 1678 GGTCTC GGTCTC(1/5)
BsmBI 326 GAGACG CGTCTC(1/5)
BspHI 1028 TCATGA T^CATGA
BsrFI 124 GCCGGC [GA]^CCGG[CT]
BssSI 1097 CACGAG CACGAG(-5/-1)
Bsu36I 970 CCTGAGG CC^T[ACGT]AGG
CfoI 80 GCGC GCG^C
Cfr10I 124 GCCGGC [GA]^CCGG[CT]
DraII 1103 GGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 628 TGGCCA [CT]^GGCC[GA]
Eco81I 970 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1103 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 723 GAATTC G^AATTC
FauI 55 CCCGC CCCGC(4/6)
HaeII 79 GGCGCC [GA]GCGC^[CT]
HhaI 80 GCGC GCG^C
Hin1I 79 GGCGCC G[GA]^CG[CT]C
HinP1I 80 GCGC G^CGC
HincII 785 GTCGAC GT[CT]^[GA]AC
HindII 785 GTCGAC GT[CT]^[GA]AC
HindIII 683 AAGCTT A^AGCTT
KasI 79 GGCGCC G^GCGCC
MaeII 1695 ACGT A^CGT
MfeI 290 CAATTG C^AATTG
MluNI 628 TGGCCA TGG^CCA
MscI 628 TGGCCA TGG^CCA
MspA1I 93 CCGCTG C[AC]G^C[GT]G
MunI 290 CAATTG C^AATTG
NaeI 124 GCCGGC GCC^GGC
NarI 79 GGCGCC GG^CGCC
NgoMIV 124 GCCGGC G^CCGGC
NspI 1074 GCATGC [GA]CATG^[CT]
PpuMI 1103 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 1028 TCATGA T^CATGA
RsaI 1507 GTAC GT^AC
SalI 785 GTCGAC G^TCGAC
SapI 586 GAAGAGC GCTCTTC(1/4)
SfoI 79 GGCGCC GGC^GCC
SphI 1074 GCATGC GCATG^C
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 100 GTCTAC GT^[AC][GT]AC
785 GTCGAC
Alw44I 16 GTGCAC G^TGCAC
531 GTGCAC
ApaLI 16 GTGCAC G^TGCAC
531 GTGCAC
AspI 749 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1487 GACTAGGTC
BanII 34 GGGCTC G[GA]GC[CT]^C
307 GAGCCC
BglI 982 GCCAAATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
998 GCCGGACAGGC
BglII 810 AGATCT A^GATCT
1457 AGATCT
BsaBI 187 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
961 GATAAAAATC
BseRI 129 CTCCTC GAGGAG(10/8)
539 CTCCTC
BsmFI 1103 GGGAC GGGAC(10/14)
574 GTCCC
DsaI 1012 CCATGG C^C[GA][CT]GG
1667 CCATGG
EarI 586 GAAGAG CTCTTC(1/4)
1708 GAAGAG
HaeIII 629 GGCC GG^CC
997 GGCC
HgaI 328 GACGC GACGC(5/10)
98 GCGTC
Ksp632I 586 GAAGAG CTCTTC(1/4)
1708 GAAGAG
MamI 187 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
961 GATAAAAATC
NcoI 1012 CCATGG C^CATGG
1667 CCATGG
PflFI 749 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1487 GACTAGGTC
PleI 462 GACTC GAGTC(4/5)
572 GAGTC
PstI 620 CTGCAG CTGCA^G
1550 CTGCAG
SfcI 620 CTGCAG C^T[GA][CT]AG
1550 CTGCAG
SmlI 175 CTTGAG C^T[CT][GA]AG
1592 CTCAAG
Tth111I 749 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1487 GACTAGGTC
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AspHI AvaII BbvI BfaI BmyI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EcoRII EcoT14I Fnu4HI FokI
HapII HinfI HpaII HphI ItaI
MaeI MboI MboII MflI MnlI
MseI MspI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII