Restriction map for
pF1KB6120
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BlnI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CfoI Cfr10I
Cfr13I CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HapII
HhaI Hin1I HinP1I HpaII KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MslI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI Sau96I SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI Tsp45I Tth111I
Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 148 GTAGAC GT^[AC][GT]AC
AflIII 1539 ACGTGT A^C[GA][CT]GT
AlwI 1125 GGATC GGATC(4/5)
AlwNI 356 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 276 ATTAAT AT^TAAT
AsnI 276 ATTAAT AT^TAAT
BanI 789 GGCACC G^G[CT][GA]CC
BbsI 1360 GAAGAC GAAGAC(2/6)
BglII 588 AGATCT A^GATCT
BlpI 40 GCTGAGC GC^T[ACGT]AGC
BmyI 959 GGGCAC G[AGT]GC[ACT]^C
BpmI 723 CTGGAG CTGGAG(16/14)
Bpu1102I 40 GCTGAGC GC^T[ACGT]AGC
BpuAI 1360 GAAGAC GAAGAC(2/6)
BseRI 931 GAGGAG GAGGAG(10/8)
BsiYI 706 CCTAAAATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 706 CCTAAAATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 297 GAGAC GTCTC(1/5)
Bsp1286I 959 GGGCAC G[AGT]GC[ACT]^C
BspDI 1729 ATCGAT AT^CGAT
BsrDI 1744 GCAATG GCAATG(2/0)
BstYI 588 AGATCT [GA]^GATC[CT]
CelII 40 GCTGAGC GC^T[ACGT]AGC
ClaI 1729 ATCGAT AT^CGAT
EcoRI 1267 GAATTC G^AATTC
FauI 351 CCCGC CCCGC(4/6)
HgaI 1176 GCGTC GACGC(5/10)
HindIII 1343 AAGCTT A^AGCTT
HpaI 699 GTTAAC GTT^AAC
HphI 99 GGTGA GGTGA(8/7)
MflI 588 AGATCT [GA]^GATC[CT]
NheI 1248 GCTAGC G^CTAGC
NlaIV 789 GGCACC GG[ACGT]^[ACGT]CC
NspI 872 ACATGC [GA]CATG^[CT]
PshBI 276 ATTAAT AT^TAAT
SexAI 92 ACCAGGT A^CC[AT]GGT
XcmI 390 CCAAGCCAAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 588 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 352 CCGC CCGC(-3/-1)
834 GCGG
AfaI 904 GTAC GT^AC
1683 GTAC
Asp700I 274 GAATTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
737 GAAATATTTC
BsaJI 721 CCCTGG C^C[ACGT][ACGT]GG
1355 CCCAGG
BstAPI 1195 GCAAAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1255 GCAGAACTTGC
BstF5I 36 GGATG GGATG(2/0)
204 GGATG
DpnII 589 GATC ^GATC
1126 GATC
FokI 36 GGATG GGATG(9/13)
204 GGATG
HaeIII 843 GGCC GG^CC
1672 GGCC
HincII 607 GTCAAC GT[CT]^[GA]AC
699 GTTAAC
HindII 607 GTCAAC GT[CT]^[GA]AC
699 GTTAAC
MboI 589 GATC ^GATC
1126 GATC
NdeII 589 GATC ^GATC
1126 GATC
PstI 139 CTGCAG CTGCA^G
362 CTGCAG
RsaI 904 GTAC GT^AC
1683 GTAC
SapI 1033 GAAGAGC GCTCTTC(1/4)
1063 GAAGAGC
Sau3AI 589 GATC ^GATC
1126 GATC
SfaNI 37 GATGC GCATC(5/9)
661 GATGC
SfcI 139 CTGCAG C^T[GA][CT]AG
362 CTGCAG
SspI 676 AATATT AAT^ATT
739 AATATT
TspRI 499 CAGTG CA[GC]TG[ACGT][ACGT]^
1272 CACTG
XmnI 274 GAATTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
737 GAAATATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AcsI AluI ApoI BbvI BfaI
BsrI BssKI BstNI Cac8I DdeI
EarI EcoRII Fnu4HI HinfI ItaI
Ksp632I MaeI MaeII MaeIII MboII
MnlI MseI MvaI MwoI NlaIII
PleI ScrFI TaqI TfiI Tru9I
TseI Tsp509I TthHB8I XbaI