Restriction map for
pF1KB6119
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# NO CUT ENZYMES ( 114 enzymes) #
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Acc65I AccI AccIII AclI AflII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BbrPI BbvCI
BcgI BcnI BfrI BglI BlnI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspMI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoT22I
FseI FspI HgaI HincII HindII
HpaI KpnI MfeI MluI MluNI
MroI MscI MunI NaeI NciI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 814 GACGTC GACGT^C
AflIII 12 ACATGT A^C[GA][CT]GT
AocI 991 CCTGAGG CC^T[ACGT]AGG
BamHI 585 GGATCC G^GATCC
BbeI 1730 GGCGCC GGCGC^C
BclI 1794 TGATCA T^GATCA
BglII 513 AGATCT A^GATCT
BlpI 1771 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1771 GCTCAGC GC^T[ACGT]AGC
BsaAI 1649 TACGTG [CT]AC^GT[GA]
BsaBI 982 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 598 GGTCTC GGTCTC(1/5)
BsaWI 834 ACCGGA [AT]^CCGG[AT]
BsmAI 599 GTCTC GTCTC(1/5)
BsmFI 325 GGGAC GGGAC(10/14)
Bsp1407I 631 TGTACA T^GTACA
BspLU11I 12 ACATGT A^CATGT
BsrBI 1780 CCGCTC CCGCTC(-3/-3)
BsrFI 1561 GCCGGT [GA]^CCGG[CT]
BsrGI 631 TGTACA T^GTACA
BstAPI 1507 GCAAGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 991 CCTGAGG CC^T[ACGT]AGG
Cac8I 1260 GCAAGC GC[ACGT]^[ACGT]GC
CelII 1771 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1561 GCCGGT [GA]^CCGG[CT]
DraII 1581 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 158 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1665 TGGCCG [CT]^GGCC[GA]
Eco81I 991 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1581 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 199 GAATTC G^AATTC
EcoRV 1364 GATATC GAT^ATC
FbaI 1794 TGATCA T^GATCA
HaeII 1730 GGCGCC [GA]GCGC^[CT]
HindIII 188 AAGCTT A^AGCTT
HphI 1792 GGTGA GGTGA(8/7)
KasI 1730 GGCGCC G^GCGCC
MamI 982 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1730 GGCGCC GG^CGCC
NcoI 41 CCATGG C^CATGG
NdeI 1312 CATATG CA^TATG
NspI 12 ACATGT [GA]CATG^[CT]
PleI 290 GAGTC GAGTC(4/5)
PpuMI 1581 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 56 CTGCAG CTGCA^G
PvuII 893 CAGCTG CAG^CTG
SapI 34 GAAGAGC GCTCTTC(1/4)
SfoI 1730 GGCGCC GGC^GCC
SspBI 631 TGTACA T^GTACA
SspI 1198 AATATT AAT^ATT
XcmI 1422 CCATAACTATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 814 GACGTC G[GA]^CG[CT]C
1730 GGCGCC
BanI 1621 GGTGCC G^G[CT][GA]CC
1730 GGCGCC
BbsI 208 GAAGAC GAAGAC(2/6)
297 GAAGAC
BciVI 45 GGATAC GTATCC(6/5)
1845 GGATAC
BpmI 443 CTCCAG CTGGAG(16/14)
1370 CTCCAG
BpuAI 208 GAAGAC GAAGAC(2/6)
297 GAAGAC
BsaHI 814 GACGTC G[GA]^CG[CT]C
1730 GGCGCC
BspHI 293 TCATGA T^CATGA
675 TCATGA
BsrDI 86 CATTGC GCAATG(2/0)
1767 CATTGC
CfoI 1692 GCGC GCG^C
1731 GCGC
DraI 192 TTTAAA TTT^AAA
238 TTTAAA
EarI 34 GAAGAG CTCTTC(1/4)
1051 GAAGAG
FauI 1550 CCCGC CCCGC(4/6)
1643 GCGGG
HaeIII 1639 GGCC GG^CC
1666 GGCC
HapII 835 CCGG C^CGG
1562 CCGG
HhaI 1692 GCGC GCG^C
1731 GCGC
Hin1I 814 GACGTC G[GA]^CG[CT]C
1730 GGCGCC
HinP1I 1692 GCGC G^CGC
1731 GCGC
HpaII 835 CCGG C^CGG
1562 CCGG
Ksp632I 34 GAAGAG CTCTTC(1/4)
1051 GAAGAG
MspI 835 CCGG C^CGG
1562 CCGG
PflMI 589 CCACTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1450 CCATACTATGG
RcaI 293 TCATGA T^CATGA
675 TCATGA
SfaNI 94 GATGC GCATC(5/9)
710 GCATC
TaqI 1335 TCGA T^CGA
1605 TCGA
Tsp45I 409 GTGAC ^GT[GC]AC
622 GTCAC
TthHB8I 1335 TCGA T^CGA
1605 TCGA
Van91I 589 CCACTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1450 CCATACTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI Asp700I AvaII BanII
BbvI BfaI BmyI BsaJI BseRI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Cfr13I
DdeI DpnII DsaI EcoRII EcoT14I
Fnu4HI FokI HinfI ItaI KspI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI MvnI MwoI NdeII NlaIII
NlaIV RsaI SacII Sau3AI Sau96I
ScrFI SfcI StyI TfiI Tru9I
TseI Tsp509I TspRI XhoII XmnI