Restriction map for
pF1KB6118
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AflIII AgeI AhdI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BbrPI BciVI BclI BfrI
BglII BlnI BsaAI BsaBI BsiWI
BsmBI BsmI Bsp1407I BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstUI BstZ17I CpoI DraI DraIII
Eam1105I EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MluI
MseI MunI MvnI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI ScaI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI SspBI SspI SwaI Tru9I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 526 GTCTAC GT^[AC][GT]AC
AocI 558 CCTCAGG CC^T[ACGT]AGG
Aor51HI 297 AGCGCT AGC^GCT
AspI 196 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1191 TGGCCA TGG^CCA
BamHI 212 GGATCC G^GATCC
BbsI 1458 GTCTTC GAAGAC(2/6)
BfaI 1216 CTAG C^TAG
BlpI 289 GCTCAGC GC^T[ACGT]AGC
BpmI 715 CTGGAG CTGGAG(16/14)
Bpu1102I 289 GCTCAGC GC^T[ACGT]AGC
BpuAI 1458 GTCTTC GAAGAC(2/6)
BsiEI 411 CGGCCG CG[GA][CT]^CG
BspDI 1207 ATCGAT AT^CGAT
BsrBI 964 CCGCTC CCGCTC(-3/-3)
BsrFI 413 GCCGGT [GA]^CCGG[CT]
BstEII 1410 GGTGACC G^GT[ACGT]ACC
BstPI 1410 GGTGACC G^GT[ACGT]ACC
BstXI 936 CCATACACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 558 CCTCAGG CC^T[ACGT]AGG
CelII 289 GCTCAGC GC^T[ACGT]AGC
Cfr10I 413 GCCGGT [GA]^CCGG[CT]
ClaI 1207 ATCGAT AT^CGAT
DrdI 60 GACCGGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1100 CCACGG C^C[GA][CT]GG
EagI 411 CGGCCG C^GGCCG
EclXI 411 CGGCCG C^GGCCG
Eco47III 297 AGCGCT AGC^GCT
Eco52I 411 CGGCCG C^GGCCG
Eco81I 558 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1410 GGTGACC G^GT[ACGT]ACC
EcoRI 1627 GAATTC G^AATTC
EcoT14I 281 CCAAGG C^C[AT][AT]GG
MaeI 1216 CTAG C^TAG
MluNI 1191 TGGCCA TGG^CCA
MscI 1191 TGGCCA TGG^CCA
MslI 1175 CATCAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 1577 ACATGC [GA]CATG^[CT]
PflFI 196 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 125 AGGACCC [GA]G^G[AT]CC[CT]
SacI 742 GAGCTC GAGCT^C
SapI 337 GAAGAGC GCTCTTC(1/4)
StyI 281 CCAAGG C^C[AT][AT]GG
Tth111I 196 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 635 CCAACCTGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 34 enzymes) #
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AccIII 581 TCCGGA T^CCGGA
992 TCCGGA
AcsI 1024 GAATTT [GA]^AATT[CT]
1627 GAATTC
Alw44I 406 GTGCAC G^TGCAC
493 GTGCAC
ApaI 985 GGGCCC GGGCC^C
1223 GGGCCC
ApaLI 406 GTGCAC G^TGCAC
493 GTGCAC
ApoI 1024 GAATTT [GA]^AATT[CT]
1627 GAATTC
BbeI 52 GGCGCC GGCGC^C
383 GGCGCC
BbvCI 1124 CCTCAGC CCTCAGC(-5/-2)
1469 CCTCAGC
BcgI 540 CGACAACTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
503 GCAAGGAAATCG
BsaI 58 GAGACC GGTCTC(1/5)
1281 GGTCTC
BseAI 581 TCCGGA T^CCGGA
992 TCCGGA
BsgI 355 CTGCAC GTGCAG(16/14)
1295 CTGCAC
BspEI 581 TCCGGA T^CCGGA
992 TCCGGA
BspMI 428 ACCTGC ACCTGC(4/8)
402 GCAGGT
BstAPI 342 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1288 GCATATTCTGC
EaeI 411 CGGCCG [CT]^GGCC[GA]
1191 TGGCCA
EarI 115 GAAGAG CTCTTC(1/4)
337 GAAGAG
HgaI 468 GACGC GACGC(5/10)
256 GCGTC
KasI 52 GGCGCC G^GCGCC
383 GGCGCC
Ksp632I 115 GAAGAG CTCTTC(1/4)
337 GAAGAG
MaeIII 680 GTGAC ^GT[ACGT]AC
1411 GTGAC
MroI 581 TCCGGA T^CCGGA
992 TCCGGA
NarI 52 GGCGCC GG^CGCC
383 GGCGCC
SbfI 180 CCTGCAGG CCTGCA^GG
318 CCTGCAGG
SfoI 52 GGCGCC GGC^GCC
383 GGCGCC
SmaI 981 CCCGGG CCC^GGG
1606 CCCGGG
Sse8387I 180 CCTGCAGG CCTGCA^GG
318 CCTGCAGG
TaqI 194 TCGA T^CGA
1208 TCGA
TfiI 576 GATTC G^A[AT]TC
1375 GATTC
Tsp45I 680 GTGAC ^GT[GC]AC
1411 GTGAC
Tsp509I 1025 AATT ^AATT
1628 AATT
TthHB8I 194 TCGA T^CGA
1208 TCGA
XmaCI 981 CCCGGG C^CCGGG
1606 CCCGGG
XmaI 981 CCCGGG C^CCGGG
1606 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbvI BcnI BglI BmyI
BsaHI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BssSI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoNI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MboI MboII MflI MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI TseI TspRI XhoII