Restriction map for
pF1KB6101
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
Acc65I AcsI AflII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaII AviII AvrII BbrPI
BbsI BclI BcnI BfrI BlnI
BpuAI BsaAI BsaI BsiWI BsmBI
Bsp1407I BspDI BspHI BspMI BsrBI
BsrDI BsrGI BssHII BstAPI BstBI
BstXI ClaI CpoI DraI DraIII
Eam1105I Eco47III EcoNI EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HindIII HpaI KpnI KspI MfeI
MluI MseI MspA1I MunI NciI
NcoI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PvuI PvuII RcaI RsrII SacII
SalI SapI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI SspBI SspI StuI StyI
SwaI Tru9I Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccIII 745 TCCGGA T^CCGGA
AclI 86 AACGTT AA^CGTT
AfaI 1554 GTAC GT^AC
AflIII 1172 ACATGT A^C[GA][CT]GT
AlwNI 1112 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 971 CCTTAGG CC^T[ACGT]AGG
ApaI 1396 GGGCCC GGGCC^C
BbeI 16 GGCGCC GGCGC^C
BbvCI 533 CCTCAGC CCTCAGC(-5/-2)
BciVI 1178 GTATCC GTATCC(6/5)
BglI 475 GCCAGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 463 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 463 GCTCAGC GC^T[ACGT]AGC
BsaBI 342 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 745 TCCGGA [AT]^CCGG[AT]
BseAI 745 TCCGGA T^CCGGA
BseRI 1670 GAGGAG GAGGAG(10/8)
BsmI 445 GCATTC GAATGC(1/-1)
BspEI 745 TCCGGA T^CCGGA
BspLU11I 1172 ACATGT A^CATGT
BssSI 814 CTCGTG CACGAG(-5/-1)
Bst1107I 830 GTATAC GTA^TAC
BstEII 955 GGTAACC G^GT[ACGT]ACC
BstPI 955 GGTAACC G^GT[ACGT]ACC
BstZ17I 830 GTATAC GTA^TAC
Bsu36I 971 CCTTAGG CC^T[ACGT]AGG
CelII 463 GCTCAGC GC^T[ACGT]AGC
Eco81I 971 CCTTAGG CC^T[ACGT]AGG
EcoO65I 955 GGTAACC G^GT[ACGT]ACC
FauI 1399 CCCGC CCCGC(4/6)
HaeII 16 GGCGCC [GA]GCGC^[CT]
HincII 1243 GTCAAC GT[CT]^[GA]AC
HindII 1243 GTCAAC GT[CT]^[GA]AC
KasI 16 GGCGCC G^GCGCC
MamI 342 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 745 TCCGGA T^CCGGA
MslI 1167 CACAGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 538 GCCGGC GCC^GGC
NarI 16 GGCGCC GG^CGCC
NdeI 920 CATATG CA^TATG
NgoMIV 538 GCCGGC G^CCGGC
NheI 738 GCTAGC G^CTAGC
NspI 1172 ACATGT [GA]CATG^[CT]
Psp1406I 86 AACGTT AA^CGTT
RsaI 1554 GTAC GT^AC
SacI 7 GAGCTC GAGCT^C
SbfI 43 CCTGCAGG CCTGCA^GG
ScaI 1553 AGTACT AGT^ACT
SfoI 16 GGCGCC GGC^GCC
Sse8387I 43 CCTGCAGG CCTGCA^GG
XcmI 1421 CCAACATCATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 850 GACGTC GACGT^C
868 GACGTC
AccI 830 GTATAC GT^[AC][GT]AC
1045 GTAGAC
AspHI 7 GAGCTC G[AT]GC[AT]^C
602 GAGCAC
AvaI 855 CCCGAG C^[CT]CG[GA]G
1667 CCCGAG
BalI 962 TGGCCA TGG^CCA
1559 TGGCCA
BamHI 1260 GGATCC G^GATCC
1567 GGATCC
BanI 16 GGCGCC G^G[CT][GA]CC
1684 GGTGCC
BfaI 280 CTAG C^TAG
739 CTAG
BglII 1310 AGATCT A^GATCT
1367 AGATCT
BsiEI 290 CGGCCG CG[GA][CT]^CG
375 CGGCCG
BsiHKAI 7 GAGCTC G[AT]GC[AT]^C
602 GAGCAC
BsmAI 755 GAGAC GTCTC(1/5)
1673 GAGAC
BsoBI 855 CCCGAG C^[CT]CG[GA]G
1667 CCCGAG
BsrFI 288 ACCGGC [GA]^CCGG[CT]
538 GCCGGC
CfoI 17 GCGC GCG^C
1691 GCGC
Cfr10I 288 ACCGGC [GA]^CCGG[CT]
538 GCCGGC
DraII 317 AGGGCCT [GA]G^G[ACGT]CC[CT]
987 AGGCCCC
DrdI 1204 GACCGCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1576 GACAAGTGCGTC
DsaI 503 CCACGG C^C[GA][CT]GG
578 CCACGG
EagI 290 CGGCCG C^GGCCG
375 CGGCCG
EclXI 290 CGGCCG C^GGCCG
375 CGGCCG
Eco52I 290 CGGCCG C^GGCCG
375 CGGCCG
EcoO109I 317 AGGGCCT [GA]G^G[ACGT]CC[CT]
987 AGGCCCC
HhaI 17 GCGC GCG^C
1691 GCGC
HinP1I 17 GCGC G^CGC
1691 GCGC
MaeI 280 CTAG C^TAG
739 CTAG
MluNI 962 TGGCCA TGG^CCA
1559 TGGCCA
MscI 962 TGGCCA TGG^CCA
1559 TGGCCA
PflMI 1250 CCATCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1376 CCATCAAGTGG
PstI 44 CTGCAG CTGCA^G
322 CTGCAG
Tsp509I 1024 AATT ^AATT
1062 AATT
Van91I 1250 CCATCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1376 CCATCAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
BanII BbvI BcgI BmyI BpmI
BsaHI BsaJI BsgI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I Cfr13I
DdeI DpnII EaeI EarI EcoRII
Fnu4HI FokI HaeIII HapII HgaI
Hin1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I XhoII