Restriction map for
pF1KB5960
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# NO CUT ENZYMES ( 101 enzymes) #
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Acc65I AccIII AcsI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AviII AvrII BbrPI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BsaI BsaWI BseAI
BsiHKAI BsiWI BsmI Bsp1407I BspDI
BspEI BsrGI BssHII Bst1107I BstAPI
BstBI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraIII Eam1105I Eco47III
Eco81I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI KspI MaeI MfeI MluI
MroI MseI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI RsrII SacI
SacII ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI Tru9I Tsp509I XbaI
XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 709 GACGTC GACGT^C
AclI 145 AACGTT AA^CGTT
AflIII 296 ACATGT A^C[GA][CT]GT
Asp700I 318 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 6 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 575 TGGCCA TGG^CCA
BamHI 611 GGATCC G^GATCC
BbeI 20 GGCGCC GGCGC^C
BglI 1391 GCCGGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 379 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 379 GCTAAGC GC^T[ACGT]AGC
BsaBI 642 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 307 GTGCAG GTGCAG(16/14)
BsiEI 1393 CGGCCG CG[GA][CT]^CG
BsmBI 1525 CGTCTC CGTCTC(1/5)
BspHI 167 TCATGA T^CATGA
BspLU11I 296 ACATGT A^CATGT
BsrDI 86 GCAATG GCAATG(2/0)
BssSI 81 CACGAG CACGAG(-5/-1)
BstEII 1067 GGTCACC G^GT[ACGT]ACC
BstPI 1067 GGTCACC G^GT[ACGT]ACC
CelII 379 GCTAAGC GC^T[ACGT]AGC
DrdI 1332 GACGCCGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1393 CGGCCG C^GGCCG
EarI 424 GAAGAG CTCTTC(1/4)
EclXI 1393 CGGCCG C^GGCCG
Eco52I 1393 CGGCCG C^GGCCG
EcoO65I 1067 GGTCACC G^GT[ACGT]ACC
KasI 20 GGCGCC G^GCGCC
Ksp632I 424 GAAGAG CTCTTC(1/4)
MamI 642 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 575 TGGCCA TGG^CCA
MscI 575 TGGCCA TGG^CCA
MslI 168 CATGAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 20 GGCGCC GG^CGCC
NspI 296 ACATGT [GA]CATG^[CT]
PflFI 6 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 145 AACGTT AA^CGTT
RcaI 167 TCATGA T^CATGA
SapI 424 GAAGAGC GCTCTTC(1/4)
SbfI 901 CCTGCAGG CCTGCA^GG
SfaNI 1608 GATGC GCATC(5/9)
SfoI 20 GGCGCC GGC^GCC
SmaI 1614 CCCGGG CCC^GGG
Sse8387I 901 CCTGCAGG CCTGCA^GG
TfiI 1112 GATTC G^A[AT]TC
Tth111I 6 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1614 CCCGGG C^CCGGG
XmaI 1614 CCCGGG C^CCGGG
XmnI 318 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 3 GTCGAC GT^[AC][GT]AC
1369 GTCGAC
AvaI 511 CCCGAG C^[CT]CG[GA]G
1614 CCCGGG
BanI 20 GGCGCC G^G[CT][GA]CC
1215 GGTGCC
BbsI 1329 GAAGAC GAAGAC(2/6)
712 GTCTTC
BbvCI 1013 CCTCAGC CCTCAGC(-5/-2)
1270 GCTGAGG
BpuAI 1329 GAAGAC GAAGAC(2/6)
712 GTCTTC
BsaAI 331 TACGTG [CT]AC^GT[GA]
1563 CACGTA
BsmAI 667 GAGAC GTCTC(1/5)
1526 GTCTC
BsoBI 511 CCCGAG C^[CT]CG[GA]G
1614 CCCGGG
BsrBI 427 GAGCGG CCGCTC(-3/-3)
607 GAGCGG
BstF5I 104 CATCC GGATG(2/0)
624 GGATG
FokI 104 CATCC GGATG(9/13)
624 GGATG
HaeII 20 GGCGCC [GA]GCGC^[CT]
1554 AGCGCC
PflMI 327 CCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1453 CCAGCCACTGG
SalI 3 GTCGAC G^TCGAC
1369 GTCGAC
SmlI 15 CTTGAG C^T[CT][GA]AG
442 CTCAAG
StuI 889 AGGCCT AGG^CCT
1421 AGGCCT
Van91I 327 CCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1453 CCAGCCACTGG
XcmI 230 CCATGACGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
449 CCAAGGAAAAGCTGG
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AvaII BanII
BbvI BcnI BmyI BpmI BsaHI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BspMI BsrFI BsrI BssKI
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfcI StyI TaqI TseI Tsp45I
TspRI TthHB8I XhoII