Restriction map for
pF1KB5955
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI Alw44I
AlwNI AocI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstUI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII EagI
EclXI Eco52I Eco81I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
Hin1I HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MspA1I MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
PvuII RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TspRI
Tth111I Van91I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
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AclI 1401 AACGTT AA^CGTT
AcsI 1259 AAATTC [GA]^AATT[CT]
AhdI 175 GACGACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 382 AGCGCT AGC^GCT
ApoI 1259 AAATTC [GA]^AATT[CT]
AspEI 175 GACGACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1348 GAGCTC G[AT]GC[AT]^C
AvaI 665 CCCGGG C^[CT]CG[GA]G
BanI 369 GGCACC G^G[CT][GA]CC
BfaI 1103 CTAG C^TAG
BsgI 591 GTGCAG GTGCAG(16/14)
BsiHKAI 1348 GAGCTC G[AT]GC[AT]^C
BsoBI 665 CCCGGG C^[CT]CG[GA]G
BspHI 815 TCATGA T^CATGA
BspMI 1037 ACCTGC ACCTGC(4/8)
BsrBI 1012 GAGCGG CCGCTC(-3/-3)
BsrI 67 CCAGT ACTGG(1/-1)
BssSI 841 CTCGTG CACGAG(-5/-1)
BstEII 861 GGTCACC G^GT[ACGT]ACC
BstPI 861 GGTCACC G^GT[ACGT]ACC
BstXI 674 CCATCAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 1034 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 1296 GACCGCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 175 GACGACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 382 AGCGCT AGC^GCT
EcoNI 1302 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1034 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 861 GGTCACC G^GT[ACGT]ACC
HindIII 426 AAGCTT A^AGCTT
MaeI 1103 CTAG C^TAG
NcoI 637 CCATGG C^CATGG
PpuMI 1034 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 1401 AACGTT AA^CGTT
PstI 826 CTGCAG CTGCA^G
RcaI 815 TCATGA T^CATGA
SacI 1348 GAGCTC GAGCT^C
SapI 930 GAAGAGC GCTCTTC(1/4)
SmaI 665 CCCGGG CCC^GGG
SmlI 33 CTTGAG C^T[CT][GA]AG
TaqI 1010 TCGA T^CGA
TthHB8I 1010 TCGA T^CGA
XmaCI 665 CCCGGG C^CCGGG
XmaI 665 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AciI 1298 CCGC CCGC(-3/-1)
1014 GCGG
AlwI 115 GATCC GGATC(4/5)
660 GATCC
AvaII 1035 GGACC G^G[AT]CC
1217 GGTCC
BalI 290 TGGCCA TGG^CCA
902 TGGCCA
BanII 874 GAGCCC G[GA]GC[CT]^C
1348 GAGCTC
BbsI 57 GAAGAC GAAGAC(2/6)
819 GAAGAC
BmyI 874 GAGCCC G[AGT]GC[ACT]^C
1348 GAGCTC
BpuAI 57 GAAGAC GAAGAC(2/6)
819 GAAGAC
BsaI 1395 GAGACC GGTCTC(1/5)
1059 GGTCTC
BseRI 217 GAGGAG GAGGAG(10/8)
871 GAGGAG
Bsp1286I 874 GAGCCC G[AGT]GC[ACT]^C
1348 GAGCTC
BstYI 114 AGATCC [GA]^GATC[CT]
659 AGATCC
CfoI 383 GCGC GCG^C
698 GCGC
Cfr13I 1035 GGACC G^G[ACGT]CC
1217 GGTCC
DpnII 115 GATC ^GATC
660 GATC
DsaI 637 CCATGG C^C[GA][CT]GG
1445 CCACGG
EaeI 290 TGGCCA [CT]^GGCC[GA]
902 TGGCCA
HaeII 382 AGCGCT [GA]GCGC^[CT]
697 GGCGCT
HgaI 349 GACGC GACGC(5/10)
1421 GCGTC
HhaI 383 GCGC GCG^C
698 GCGC
HinP1I 383 GCGC G^CGC
698 GCGC
MaeII 1289 ACGT A^CGT
1402 ACGT
MboI 115 GATC ^GATC
660 GATC
MflI 114 AGATCC [GA]^GATC[CT]
659 AGATCC
MluNI 290 TGGCCA TGG^CCA
902 TGGCCA
MscI 290 TGGCCA TGG^CCA
902 TGGCCA
MslI 463 CATGGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1070 CACTCATGTG
NdeII 115 GATC ^GATC
660 GATC
Sau3AI 115 GATC ^GATC
660 GATC
Sau96I 1035 GGACC G^G[ACGT]CC
1217 GGTCC
SfcI 826 CTGCAG C^T[GA][CT]AG
1314 CTACAG
XcmI 278 CCAGCAACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
628 CCACAGAAGCCATGG
XhoII 114 AGATCC [GA]^GATC[CT]
659 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AfaI AluI BbvI BcnI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
BssKI BstF5I BstNI Cac8I DdeI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeIII MboII MnlI
MseI MspI MvaI MwoI NciI
NlaIII NlaIV PleI RsaI ScrFI
SfaNI StyI TfiI Tru9I TseI
Tsp45I Tsp509I