Restriction map for
pF1KB5912
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# NO CUT ENZYMES ( 139 enzymes) #
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AatII Acc65I AccI AccII AccIII
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaBI BsaI BsaWI BseAI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DraI
DraIII DrdI DsaI Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HhaI HinP1I HpaI KasI
KpnI KspI MamI MflI MluI
MluNI MroI MscI MvnI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SpeI SphI Sse8387I SspBI StuI
SwaI TaqI TthHB8I Van91I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AclI 1513 AACGTT AA^CGTT
AcsI 367 AAATTT [GA]^AATT[CT]
AcyI 83 GGCGTC G[GA]^CG[CT]C
AlwI 790 GATCC GGATC(4/5)
ApoI 367 AAATTT [GA]^AATT[CT]
Asp700I 1479 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1004 GAGCAC G[AT]GC[AT]^C
BanI 484 GGTGCC G^G[CT][GA]CC
BbsI 38 GTCTTC GAAGAC(2/6)
BbvCI 111 GCTGAGG CCTCAGC(-5/-2)
BcnI 348 CCGGG CC^[GC]GG
BpuAI 38 GTCTTC GAAGAC(2/6)
BsaHI 83 GGCGTC G[GA]^CG[CT]C
BseRI 147 GAGGAG GAGGAG(10/8)
BsiEI 1092 CGGCCG CG[GA][CT]^CG
BsiHKAI 1004 GAGCAC G[AT]GC[AT]^C
BsmAI 590 GAGAC GTCTC(1/5)
BspHI 783 TCATGA T^CATGA
BsrFI 1090 GCCGGC [GA]^CCGG[CT]
BstXI 464 CCAAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1090 GCCGGC [GA]^CCGG[CT]
DraII 1573 GGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 1092 CGGCCG [CT]^GGCC[GA]
EagI 1092 CGGCCG C^GGCCG
EclXI 1092 CGGCCG C^GGCCG
Eco52I 1092 CGGCCG C^GGCCG
EcoO109I 1573 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 464 CCAAGG C^C[AT][AT]GG
HgaI 84 GCGTC GACGC(5/10)
Hin1I 83 GGCGTC G[GA]^CG[CT]C
HincII 616 GTTGAC GT[CT]^[GA]AC
HindII 616 GTTGAC GT[CT]^[GA]AC
MfeI 923 CAATTG C^AATTG
MunI 923 CAATTG C^AATTG
NaeI 1090 GCCGGC GCC^GGC
NciI 348 CCGGG CC^[GC]GG
NgoMIV 1090 GCCGGC G^CCGGC
NspI 664 GCATGT [GA]CATG^[CT]
PpuMI 1573 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 1513 AACGTT AA^CGTT
PstI 190 CTGCAG CTGCA^G
PvuII 29 CAGCTG CAG^CTG
RcaI 783 TCATGA T^CATGA
SmlI 290 CTCAAG C^T[CT][GA]AG
SnaBI 677 TACGTA TAC^GTA
SspI 955 AATATT AAT^ATT
StyI 464 CCAAGG C^C[AT][AT]GG
XmnI 1479 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwNI 136 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
641 CAGTTCCTG
AspI 238 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1071 GACAATGTC
BanII 551 GGGCTC G[GA]GC[CT]^C
1565 GGGCTC
BsaAI 677 TACGTA [CT]AC^GT[GA]
1065 TACGTG
BsgI 725 CTGCAC GTGCAG(16/14)
611 GTGCAG
BsmI 15 GAATGC GAATGC(1/-1)
496 GCATTC
BspMI 811 ACCTGC ACCTGC(4/8)
568 GCAGGT
BsrI 964 ACTGG ACTGG(1/-1)
1214 CCAGT
Cac8I 1025 GCCTGC GC[ACGT]^[ACGT]GC
1090 GCCGGC
DpnII 790 GATC ^GATC
1507 GATC
EarI 144 GAAGAG CTCTTC(1/4)
1498 GAAGAG
HaeIII 1093 GGCC GG^CC
1197 GGCC
HapII 348 CCGG C^CGG
1091 CCGG
HindIII 898 AAGCTT A^AGCTT
1467 AAGCTT
HpaII 348 CCGG C^CGG
1091 CCGG
Ksp632I 144 GAAGAG CTCTTC(1/4)
1498 GAAGAG
MboI 790 GATC ^GATC
1507 GATC
MspA1I 29 CAGCTG C[AC]G^C[GT]G
187 CCGCTG
MspI 348 CCGG C^CGG
1091 CCGG
NdeII 790 GATC ^GATC
1507 GATC
PflFI 238 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1071 GACAATGTC
Sau3AI 790 GATC ^GATC
1507 GATC
SfcI 190 CTGCAG C^T[GA][CT]AG
1490 CTGTAG
Tth111I 238 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1071 GACAATGTC
XcmI 1301 CCAAGATTATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1551 CCAAGTTCAGGATGG
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# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BfaI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrDI BssKI BstF5I
BstNI Cfr13I DdeI EcoRII Fnu4HI
FokI HinfI HphI ItaI MaeI
MaeII MaeIII MboII MnlI MseI
MslI MvaI MwoI NlaIII NlaIV
PleI RsaI Sau96I ScrFI SfaNI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI