Restriction map for
pF1KB7968
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII BanI BbeI BbsI
BbvCI BciVI BclI BfrI BglI
BpuAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspEI BspHI BspLU11I BsrGI BssHII
Bst1107I BstBI BstUI BstZ17I Bsu36I
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRV EcoT22I FbaI FseI
FspI Hin1I HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcsI 1011 GAATTC [GA]^AATT[CT]
AflIII 1061 ACACGT A^C[GA][CT]GT
ApaI 93 GGGCCC GGGCC^C
ApoI 1011 GAATTC [GA]^AATT[CT]
AvaII 175 GGACC G^G[AT]CC
AvrII 496 CCTAGG C^CTAGG
BalI 1152 TGGCCA TGG^CCA
BamHI 1121 GGATCC G^GATCC
BcgI 202 CGACAGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 297 CCCGG CC^[GC]GG
BglII 504 AGATCT A^GATCT
BlnI 496 CCTAGG C^CTAGG
BsaI 1053 GAGACC GGTCTC(1/5)
BspDI 323 ATCGAT AT^CGAT
BspMI 786 GCAGGT ACCTGC(4/8)
BsrBI 957 CCGCTC CCGCTC(-3/-3)
BsrDI 835 CATTGC GCAATG(2/0)
BsrFI 554 ACCGGC [GA]^CCGG[CT]
BssSI 358 CTCGTG CACGAG(-5/-1)
BstAPI 423 GCAAAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 943 GGTCACC G^GT[ACGT]ACC
BstPI 943 GGTCACC G^GT[ACGT]ACC
BstXI 391 CCAAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 706 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 187 GCGC GCG^C
Cfr10I 554 ACCGGC [GA]^CCGG[CT]
ClaI 323 ATCGAT AT^CGAT
DsaI 1212 CCATGG C^C[GA][CT]GG
EcoO65I 943 GGTCACC G^GT[ACGT]ACC
EcoRI 1011 GAATTC G^AATTC
FauI 956 CCCGC CCCGC(4/6)
HaeII 186 GGCGCT [GA]GCGC^[CT]
HgaI 149 GACGC GACGC(5/10)
HhaI 187 GCGC GCG^C
HinP1I 187 GCGC G^CGC
HincII 1098 GTCAAC GT[CT]^[GA]AC
HindII 1098 GTCAAC GT[CT]^[GA]AC
MluNI 1152 TGGCCA TGG^CCA
MscI 1152 TGGCCA TGG^CCA
MseI 25 TTAA T^TAA
MslI 1142 CATACCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 704 CAGCTG C[AC]G^C[GT]G
NciI 297 CCCGG CC^[GC]GG
NcoI 1212 CCATGG C^CATGG
PstI 730 CTGCAG CTGCA^G
PvuII 704 CAGCTG CAG^CTG
SfcI 730 CTGCAG C^T[GA][CT]AG
TaqI 324 TCGA T^CGA
TfiI 735 GAATC G^A[AT]TC
Tru9I 25 TTAA T^TAA
TthHB8I 324 TCGA T^CGA
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Asp700I 364 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
996 GAAAAGCTTC
AvaI 229 CCCGAG C^[CT]CG[GA]G
311 CTCGGG
BbrPI 1005 CACGTG CAC^GTG
1062 CACGTG
BfaI 79 CTAG C^TAG
497 CTAG
BlpI 558 GCTGAGC GC^T[ACGT]AGC
959 GCTCAGC
BpmI 521 CTCCAG CTGGAG(16/14)
1130 CTGGAG
Bpu1102I 558 GCTGAGC GC^T[ACGT]AGC
959 GCTCAGC
BsaAI 1005 CACGTG [CT]AC^GT[GA]
1062 CACGTG
BseRI 98 CTCCTC GAGGAG(10/8)
589 CTCCTC
BsmFI 619 GGGAC GGGAC(10/14)
1217 GGGAC
BsoBI 229 CCCGAG C^[CT]CG[GA]G
311 CTCGGG
BstF5I 288 CATCC GGATG(2/0)
380 CATCC
BstNI 306 CCAGG CC^[AT]GG
537 CCAGG
CelII 558 GCTGAGC GC^T[ACGT]AGC
959 GCTCAGC
EaeI 404 TGGCCG [CT]^GGCC[GA]
1152 TGGCCA
EarI 82 GAAGAG CTCTTC(1/4)
1023 GAAGAG
EcoRII 306 CCAGG ^CC[AT]GG
537 CCAGG
FokI 288 CATCC GGATG(9/13)
380 CATCC
HapII 298 CCGG C^CGG
555 CCGG
HpaII 298 CCGG C^CGG
555 CCGG
Ksp632I 82 GAAGAG CTCTTC(1/4)
1023 GAAGAG
MaeI 79 CTAG C^TAG
497 CTAG
MaeII 1006 ACGT A^CGT
1063 ACGT
MspI 298 CCGG C^CGG
555 CCGG
MvaI 306 CCAGG CC^[AT]GG
537 CCAGG
NlaIII 692 CATG CATG^
1213 CATG
PmaCI 1005 CACGTG CAC^GTG
1062 CACGTG
PmlI 1005 CACGTG CAC^GTG
1062 CACGTG
Tsp509I 611 AATT ^AATT
1012 AATT
XmnI 364 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
996 GAAAAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
BanII BbvI BmyI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstYI Cfr13I DdeI
DpnII DraII EcoO109I EcoT14I Fnu4HI
HaeIII HindIII HinfI HphI ItaI
MaeIII MboI MboII MflI MnlI
MwoI NdeII NlaIV PleI SacI
Sau3AI Sau96I ScrFI SfaNI StyI
TseI Tsp45I TspRI XhoII