Restriction map for
pF1KB7948
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BpmI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstBI
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoRI EcoRII EcoT22I
FauI FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HindIII KasI
KpnI Ksp632I KspI MaeI MamI
MflI MluI MluNI MroI MscI
MvaI MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 961 GTATAC GT^[AC][GT]AC
AflIII 801 ACATGT A^C[GA][CT]GT
AlwNI 182 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 1037 GTGCTC G[AT]GC[AT]^C
AvaI 377 CCCGGG C^[CT]CG[GA]G
AvaII 97 GGACC G^G[AT]CC
BbsI 7 GAAGAC GAAGAC(2/6)
BlpI 1039 GCTCAGC GC^T[ACGT]AGC
BmyI 1037 GTGCTC G[AGT]GC[ACT]^C
Bpu1102I 1039 GCTCAGC GC^T[ACGT]AGC
BpuAI 7 GAAGAC GAAGAC(2/6)
BsaAI 34 TACGTA [CT]AC^GT[GA]
BsiHKAI 1037 GTGCTC G[AT]GC[AT]^C
BsmAI 663 GAGAC GTCTC(1/5)
BsoBI 377 CCCGGG C^[CT]CG[GA]G
Bsp1286I 1037 GTGCTC G[AGT]GC[ACT]^C
BspLU11I 801 ACATGT A^CATGT
BspMI 531 ACCTGC ACCTGC(4/8)
BsrDI 137 GCAATG GCAATG(2/0)
Bst1107I 961 GTATAC GTA^TAC
BstEII 40 GGTAACC G^GT[ACGT]ACC
BstPI 40 GGTAACC G^GT[ACGT]ACC
BstXI 891 CCAAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 961 GTATAC GTA^TAC
CelII 1039 GCTCAGC GC^T[ACGT]AGC
DsaI 973 CCATGG C^C[GA][CT]GG
EcoNI 1045 CCTCCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 40 GGTAACC G^GT[ACGT]ACC
EcoRV 334 GATATC GAT^ATC
HgaI 637 GCGTC GACGC(5/10)
HpaI 645 GTTAAC GTT^AAC
MaeII 35 ACGT A^CGT
MfeI 510 CAATTG C^AATTG
MspA1I 182 CAGCTG C[AC]G^C[GT]G
MunI 510 CAATTG C^AATTG
NcoI 973 CCATGG C^CATGG
NdeI 469 CATATG CA^TATG
NspI 801 ACATGT [GA]CATG^[CT]
PvuII 182 CAGCTG CAG^CTG
SfcI 867 CTATAG C^T[GA][CT]AG
SmaI 377 CCCGGG CCC^GGG
SnaBI 34 TACGTA TAC^GTA
Tsp45I 58 GTGAC ^GT[GC]AC
TspRI 481 CAGTG CA[GC]TG[ACGT][ACGT]^
XmaCI 377 CCCGGG C^CCGGG
XmaI 377 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 949 CCGC CCGC(-3/-1)
476 GCGG
AcsI 160 GAATTT [GA]^AATT[CT]
215 AAATTT
ApoI 160 GAATTT [GA]^AATT[CT]
215 AAATTT
BseRI 1061 CTCCTC GAGGAG(10/8)
1079 CTCCTC
BsgI 533 CTGCAC GTGCAG(16/14)
755 GTGCAG
DdeI 563 CTAAG C^T[ACGT]AG
1040 CTCAG
DpnII 652 GATC ^GATC
1000 GATC
EcoT14I 973 CCATGG C^C[AT][AT]GG
1050 CCAAGG
HaeIII 516 GGCC GG^CC
880 GGCC
HincII 645 GTTAAC GT[CT]^[GA]AC
862 GTTGAC
HindII 645 GTTAAC GT[CT]^[GA]AC
862 GTTGAC
MboI 652 GATC ^GATC
1000 GATC
NdeII 652 GATC ^GATC
1000 GATC
PleI 27 GACTC GAGTC(4/5)
312 GAGTC
PshAI 60 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
865 GACTATAGTC
Sau3AI 652 GATC ^GATC
1000 GATC
StyI 973 CCATGG C^C[AT][AT]GG
1050 CCAAGG
TfiI 829 GAATC G^A[AT]TC
978 GAATC
XcmI 291 CCATGTGTTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1089 CCAAGCCGGATATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AfaI AluI BbvI BcnI BsaJI
BsiYI BslI BsrI BssKI BstAPI
BstF5I Cfr13I Fnu4HI FokI HapII
HinfI HpaII HphI ItaI MaeIII
MboII MnlI MseI MslI MspI
MwoI NciI NlaIII NlaIV RsaI
Sau96I ScrFI SfaNI Tru9I TseI
Tsp509I