Restriction map for
pF1KB7925
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# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BanI
BbeI BbrPI BbsI BcgI BciVI
BclI BcnI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII HphI KasI
KpnI KspI MaeI MaeII MamI
MfeI MluI MroI MseI MslI
MspI MunI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcsI 36 GAATTT [GA]^AATT[CT]
AfaI 182 GTAC GT^AC
ApaI 236 GGGCCC GGGCC^C
ApoI 36 GAATTT [GA]^AATT[CT]
AspHI 126 GAGCTC G[AT]GC[AT]^C
AvaII 318 GGTCC G^G[AT]CC
BalI 150 TGGCCA TGG^CCA
BamHI 225 GGATCC G^GATCC
BbvCI 385 GCTGAGG CCTCAGC(-5/-2)
BglI 382 GCCGCTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 94 GAGACC GGTCTC(1/5)
BseRI 118 GAGGAG GAGGAG(10/8)
BsgI 442 CTGCAC GTGCAG(16/14)
BsiHKAI 126 GAGCTC G[AT]GC[AT]^C
BsmFI 319 GTCCC GGGAC(10/14)
BstYI 225 GGATCC [GA]^GATC[CT]
EaeI 150 TGGCCA [CT]^GGCC[GA]
EarI 299 CTCTTC CTCTTC(1/4)
EcoT22I 558 ATGCAT ATGCA^T
Ksp632I 299 CTCTTC CTCTTC(1/4)
MaeIII 190 GTGAC ^GT[ACGT]AC
MflI 225 GGATCC [GA]^GATC[CT]
MluNI 150 TGGCCA TGG^CCA
MscI 150 TGGCCA TGG^CCA
NdeI 255 CATATG CA^TATG
NlaIII 81 CATG CATG^
NsiI 558 ATGCAT ATGCA^T
NspI 80 ACATGC [GA]CATG^[CT]
PflMI 336 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 317 AGGTCCC [GA]G^G[AT]CC[CT]
RsaI 182 GTAC GT^AC
SacI 126 GAGCTC GAGCT^C
SmlI 570 CTTGAG C^T[CT][GA]AG
TfiI 485 GATTC G^A[AT]TC
Tsp45I 190 GTGAC ^GT[GC]AC
Tsp509I 37 AATT ^AATT
Van91I 336 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 225 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 202 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
464 CAGCTCCTG
AvaI 91 CCCGAG C^[CT]CG[GA]G
115 CCCGAG
BsoBI 91 CCCGAG C^[CT]CG[GA]G
115 CCCGAG
BstF5I 24 CATCC GGATG(2/0)
556 GGATG
Cac8I 519 GCCAGC GC[ACGT]^[ACGT]GC
523 GCCAGC
DpnII 226 GATC ^GATC
270 GATC
EcoT14I 132 CCAAGG C^C[AT][AT]GG
194 CCTTGG
FokI 24 CATCC GGATG(9/13)
556 GGATG
MboI 226 GATC ^GATC
270 GATC
MboII 104 TCTTC GAAGA(8/7)
300 TCTTC
MspA1I 367 CAGCGG C[AC]G^C[GT]G
383 CCGCTG
MwoI 366 GCAGCGGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
382 GCCGCTGAGGC
NdeII 226 GATC ^GATC
270 GATC
PleI 399 GACTC GAGTC(4/5)
430 GACTC
PstI 85 CTGCAG CTGCA^G
307 CTGCAG
Sau3AI 226 GATC ^GATC
270 GATC
SfaNI 557 GATGC GCATC(5/9)
560 GCATC
SfcI 85 CTGCAG C^T[GA][CT]AG
307 CTGCAG
StyI 132 CCAAGG C^C[AT][AT]GG
194 CCTTGG
TspRI 63 CACTG CA[GC]TG[ACGT][ACGT]^
188 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AluI AlwI BanII BbvI
BmyI BpmI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstNI
Cfr13I DdeI DraII EcoO109I EcoRII
Fnu4HI HaeIII HinfI ItaI MnlI
MvaI NlaIV Sau96I ScrFI TseI