##################################
# NO CUT ENZYMES (  204 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmFI       BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspMI       
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstNI       
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       Cfr10I      Cfr13I      ClaI        
CpoI        DpnII       DraI        DraII       DraIII      
DrdI        DsaI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HgaI        Hin1I       HincII      HindII      
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MamI        MboI        MboII       
MfeI        MflI        MluI        MluNI       MroI        
MscI        MslI        MunI        MvaI        MvnI        
NaeI        NarI        NcoI        NdeI        NdeII       
NgoMIV      NheI        NlaIV       NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
Sau96I      SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tsp45I      
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AfaI          204  GTAC                 GT^AC
AflIII        221  ACATGT               A^C[GA][CT]GT
BcnI          326  CCCGG                CC^[GC]GG
BsaJI         353  CCTTGG               C^C[ACGT][ACGT]GG
BsiYI         326  CCCGGAAGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          326  CCCGGAAGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI          22  GAGAC                GTCTC(1/5)
BsmI          267  GAATGC               GAATGC(1/-1)
BspLU11I      221  ACATGT               A^CATGT
BsrDI          75  GCAATG               GCAATG(2/0)
BsrI          170  ACTGG                ACTGG(1/-1)
BssKI         326  CCCGG                ^CC[ACGT]GG
BstF5I        335  GGATG                GGATG(2/0)
Cac8I         348  GCAAGC               GC[ACGT]^[ACGT]GC
CfoI          339  GCGC                 GCG^C
EaeI            2  TGGCCG               [CT]^GGCC[GA]
EcoT14I       353  CCTTGG               C^C[AT][AT]GG
FokI          335  GGATG                GGATG(9/13)
HaeIII          3  GGCC                 GG^CC
HapII         327  CCGG                 C^CGG
HhaI          339  GCGC                 GCG^C
HinP1I        339  GCGC                 G^CGC
HindIII       137  AAGCTT               A^AGCTT
HinfI         303  GATTC                G^A[ACGT]TC
HpaII         327  CCGG                 C^CGG
HphI          264  GGTGA                GGTGA(8/7)
MaeIII        122  GTTAC                ^GT[ACGT]AC
MspA1I          5  CCGCTG               C[AC]G^C[GT]G
MspI          327  CCGG                 C^CGG
NciI          326  CCCGG                CC^[GC]GG
NlaIII        222  CATG                 CATG^
NspI          221  ACATGT               [GA]CATG^[CT]
RsaI          204  GTAC                 GT^AC
ScrFI         326  CCCGG                CC^[ACGT]GG
StyI          353  CCTTGG               C^C[AT][AT]GG
TaqI           42  TCGA                 T^CGA
TfiI          303  GATTC                G^A[AT]TC
TthHB8I        42  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

AciI            5  CCGC                 CCGC(-3/-1)
              333  GCGG                
AcsI           67  GAATTT               [GA]^AATT[CT]
              283  AAATTT              
ApoI           67  GAATTT               [GA]^AATT[CT]
              283  AAATTT              
DdeI          209  CTCAG                C^T[ACGT]AG
              272  CTCAG               
MnlI          208  CCTC                 CCTC(7/6)
              119  GAGG                
SfaNI          31  GATGC                GCATC(5/9)
              336  GATGC               
TspRI         211  CAGTG                CA[GC]TG[ACGT][ACGT]^
              274  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (    9 enzymes) #
#########################################

AluI        BbvI        Fnu4HI      ItaI        MseI        
MwoI        Tru9I       TseI        Tsp509I