Restriction map for
pF1KB7650
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AfaI AflII AflIII AgeI AhdI
AlwI AocI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BanII BbeI
BbrPI BbsI BbvCI BcnI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssHII BssKI BssSI Bst1107I
BstBI BstNI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I Eco81I EcoRI
EcoRII EcoRV EcoT22I FspI HgaI
Hin1I HincII HindII HpaI KasI
KpnI MamI MfeI MflI MluI
MroI MunI MvaI NarI NciI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
RcaI RsaI RsrII SacI SalI
SbfI ScaI ScrFI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 734 GTCTAC GT^[AC][GT]AC
AccII 127 CGCG CG^CG
AcsI 418 AAATTC [GA]^AATT[CT]
Alw44I 215 GTGCAC G^TGCAC
Aor51HI 56 AGCGCT AGC^GCT
ApaLI 215 GTGCAC G^TGCAC
ApoI 418 AAATTC [GA]^AATT[CT]
AspHI 215 GTGCAC G[AT]GC[AT]^C
AvaI 70 CCCGAG C^[CT]CG[GA]G
BalI 776 TGGCCA TGG^CCA
BanI 1126 GGTGCC G^G[CT][GA]CC
BcgI 167 GCATCTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 303 GTATCC GTATCC(6/5)
BfaI 853 CTAG C^TAG
BglI 769 GCCAAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 215 GTGCAC G[AGT]GC[ACT]^C
BseRI 934 CTCCTC GAGGAG(10/8)
BsgI 1089 CTGCAC GTGCAG(16/14)
BsiHKAI 215 GTGCAC G[AT]GC[AT]^C
BsmAI 745 GTCTC GTCTC(1/5)
BsoBI 70 CCCGAG C^[CT]CG[GA]G
Bsp1286I 215 GTGCAC G[AGT]GC[ACT]^C
BspMI 1087 ACCTGC ACCTGC(4/8)
BsrDI 1121 GCAATG GCAATG(2/0)
BstAPI 994 GCACGGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 127 CGCG CG^CG
DsaI 126 CCGCGG C^C[GA][CT]GG
Eco47III 56 AGCGCT AGC^GCT
EcoT14I 496 CCTTGG C^C[AT][AT]GG
FseI 101 GGCCGGCC GGCCGG^CC
HaeII 56 AGCGCT [GA]GCGC^[CT]
HindIII 27 AAGCTT A^AGCTT
KspI 126 CCGCGG CCGC^GG
MaeI 853 CTAG C^TAG
MaeII 77 ACGT A^CGT
MluNI 776 TGGCCA TGG^CCA
MscI 776 TGGCCA TGG^CCA
MseI 626 TTAA T^TAA
MslI 648 CATGTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 127 CGCG CG^CG
PpuMI 18 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 238 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 340 CAGCTG CAG^CTG
SacII 126 CCGCGG CCGC^GG
SapI 186 GCTCTTC GCTCTTC(1/4)
SfcI 958 CTACAG C^T[GA][CT]AG
StyI 496 CCTTGG C^C[AT][AT]GG
Tru9I 626 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwNI 961 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1158 CAGCTACTG
BsaJI 126 CCGCGG C^C[ACGT][ACGT]GG
496 CCTTGG
BsiEI 104 CGGCCG CG[GA][CT]^CG
129 CGGCCG
BsrFI 102 GCCGGC [GA]^CCGG[CT]
131 GCCGGC
BsrI 774 ACTGG ACTGG(1/-1)
1163 ACTGG
BstEII 118 GGTTACC G^GT[ACGT]ACC
666 GGTCACC
BstPI 118 GGTTACC G^GT[ACGT]ACC
666 GGTCACC
CfoI 13 GCGC GCG^C
57 GCGC
Cfr10I 102 GCCGGC [GA]^CCGG[CT]
131 GCCGGC
DraII 18 GGGACCC [GA]G^G[ACGT]CC[CT]
326 AGGGCCT
EagI 104 CGGCCG C^GGCCG
129 CGGCCG
EarI 187 CTCTTC CTCTTC(1/4)
1171 GAAGAG
EclXI 104 CGGCCG C^GGCCG
129 CGGCCG
Eco52I 104 CGGCCG C^GGCCG
129 CGGCCG
EcoNI 273 CCTTGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1013 CCTTGATCAGG
EcoO109I 18 GGGACCC [GA]G^G[ACGT]CC[CT]
326 AGGGCCT
EcoO65I 118 GGTTACC G^GT[ACGT]ACC
666 GGTCACC
HapII 103 CCGG C^CGG
132 CCGG
HhaI 13 GCGC GCG^C
57 GCGC
HinP1I 13 GCGC G^CGC
57 GCGC
HpaII 103 CCGG C^CGG
132 CCGG
Ksp632I 187 CTCTTC CTCTTC(1/4)
1171 GAAGAG
MspI 103 CCGG C^CGG
132 CCGG
NaeI 102 GCCGGC GCC^GGC
131 GCCGGC
NgoMIV 102 GCCGGC G^CCGGC
131 GCCGGC
NspI 431 GCATGT [GA]CATG^[CT]
969 GCATGT
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI AvaII BbvI BclI
BpmI BsiYI BslI BsmFI BstF5I
Cac8I Cfr13I DdeI DpnII EaeI
FauI FbaI Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeIII MboI
MboII MnlI MspA1I MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I SfaNI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I