Restriction map for
pF1KB9882
##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AclI AcsI AgeI
AhdI Alw44I ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BbsI BbvCI
BcgI BclI BglII BlnI BpuAI
BsaBI BsiWI Bsp1407I BspLU11I BspMI
BsrGI Bst1107I BstBI BstZ17I CpoI
DraI DraIII Eam1105I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI KspI MamI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tsp509I Tth111I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 1354 GTCTAC GT^[AC][GT]AC
AccIII 726 TCCGGA T^CCGGA
AfaI 486 GTAC GT^AC
AflII 1603 CTTAAG C^TTAAG
AflIII 581 ACGTGT A^C[GA][CT]GT
AocI 474 CCTGAGG CC^T[ACGT]AGG
Aor51HI 632 AGCGCT AGC^GCT
ApaI 133 GGGCCC GGGCC^C
BamHI 1681 GGATCC G^GATCC
BbrPI 987 CACGTG CAC^GTG
BfrI 1603 CTTAAG C^TTAAG
BlpI 123 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 123 GCTCAGC GC^T[ACGT]AGC
BsaAI 987 CACGTG [CT]AC^GT[GA]
BsaWI 726 TCCGGA [AT]^CCGG[AT]
BseAI 726 TCCGGA T^CCGGA
BsgI 349 CTGCAC GTGCAG(16/14)
BsmI 1713 GAATGC GAATGC(1/-1)
BspDI 1078 ATCGAT AT^CGAT
BspEI 726 TCCGGA T^CCGGA
BspHI 1067 TCATGA T^CATGA
BsrBI 940 CCGCTC CCGCTC(-3/-3)
BsrDI 1521 CATTGC GCAATG(2/0)
BssHII 164 GCGCGC G^CGCGC
BstAPI 1717 GCAGACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1425 GGTCACC G^GT[ACGT]ACC
BstPI 1425 GGTCACC G^GT[ACGT]ACC
Bsu36I 474 CCTGAGG CC^T[ACGT]AGG
CelII 123 GCTCAGC GC^T[ACGT]AGC
ClaI 1078 ATCGAT AT^CGAT
DrdI 1645 GACAGAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 268 CGGCCG C^GGCCG
EclXI 268 CGGCCG C^GGCCG
Eco47III 632 AGCGCT AGC^GCT
Eco52I 268 CGGCCG C^GGCCG
Eco81I 474 CCTGAGG CC^T[ACGT]AGG
EcoNI 1286 CCTCCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1425 GGTCACC G^GT[ACGT]ACC
HincII 565 GTCAAC GT[CT]^[GA]AC
HindII 565 GTCAAC GT[CT]^[GA]AC
MroI 726 TCCGGA T^CCGGA
MslI 1077 CATCGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 626 CCATGG C^CATGG
PflMI 327 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 987 CACGTG CAC^GTG
PmlI 987 CACGTG CAC^GTG
PstI 1258 CTGCAG CTGCA^G
RcaI 1067 TCATGA T^CATGA
RsaI 486 GTAC GT^AC
SapI 1609 GAAGAGC GCTCTTC(1/4)
ScaI 485 AGTACT AGT^ACT
SfcI 1258 CTGCAG C^T[GA][CT]AG
SfiI 260 GGCCTCGACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1603 CTTAAG C^T[CT][GA]AG
StuI 1283 AGGCCT AGG^CCT
Tsp45I 1426 GTCAC ^GT[GC]AC
Van91I 327 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AspHI 97 GAGCTC G[AT]GC[AT]^C
732 GAGCTC
BalI 1088 TGGCCA TGG^CCA
1187 TGGCCA
BbeI 147 GGCGCC GGCGC^C
858 GGCGCC
BciVI 521 GTATCC GTATCC(6/5)
723 GTATCC
BfaI 1394 CTAG C^TAG
1747 CTAG
BsaI 808 GAGACC GGTCTC(1/5)
982 GAGACC
BsiEI 268 CGGCCG CG[GA][CT]^CG
495 CGACCG
BsiHKAI 97 GAGCTC G[AT]GC[AT]^C
732 GAGCTC
BssSI 1585 CACGAG CACGAG(-5/-1)
1018 CTCGTG
BstXI 950 CCAGGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1178 CCATGTCCCTGG
BstYI 1262 AGATCC [GA]^GATC[CT]
1681 GGATCC
DsaI 256 CCACGG C^C[GA][CT]GG
626 CCATGG
KasI 147 GGCGCC G^GCGCC
858 GGCGCC
MaeI 1394 CTAG C^TAG
1747 CTAG
MaeIII 1426 GTCAC ^GT[ACGT]AC
1757 GTAAC
MflI 1262 AGATCC [GA]^GATC[CT]
1681 GGATCC
MluNI 1088 TGGCCA TGG^CCA
1187 TGGCCA
MscI 1088 TGGCCA TGG^CCA
1187 TGGCCA
MseI 659 TTAA T^TAA
1604 TTAA
NaeI 144 GCCGGC GCC^GGC
1593 GCCGGC
NarI 147 GGCGCC GG^CGCC
858 GGCGCC
NgoMIV 144 GCCGGC G^CCGGC
1593 GCCGGC
SacI 97 GAGCTC GAGCT^C
732 GAGCTC
SfaNI 1226 GCATC GCATC(5/9)
1397 GCATC
SfoI 147 GGCGCC GGC^GCC
858 GGCGCC
SmaI 65 CCCGGG CCC^GGG
216 CCCGGG
Tru9I 659 TTAA T^TAA
1604 TTAA
XhoII 1262 AGATCC [GA]^GATC[CT]
1681 GGATCC
XmaCI 65 CCCGGG C^CCGGG
216 CCCGGG
XmaI 65 CCCGGG C^CCGGG
216 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI AvaI AvaII BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaHI BsaJI BseRI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PvuII Sau3AI
Sau96I ScrFI StyI TaqI TseI
TspRI TthHB8I XcmI